Cloning
cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1
Authors: W.-R. Hou Y.-L. Hou X. Ding T. Wang
The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.3.5
- DOI:
- 10.4238/2012.September.3.5
Cloning of a phosphatidylinositol 4-kinase gene based on fiber strength transcriptome QTL mapping in the cotton species Gossypium barbadense
Authors: H.W. Liu R.F. Shi X.F. Wang Y.X. Pan G.Y. Zang Z.Y. Ma
Sea Island cotton (Gossypium barbadense) is highly valued for its superior fiber qualities, especially fiber strength. Based on a transcript-derived fragment originated from transcriptome QTL mapping, a fiber strength related candidate gene of phosphatidylinositol 4-kinase cDNA, designated as GbPI4K, was first cloned, and its expression was characterized in .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.13.1
- DOI:
- 10.4238/2012.July.13.1
Molecular cloning and characterization of the pseudorabies virus US1 gene
Authors: M.L. Li J.H. Chen Z.Y. Zhao K.J. Zhang Z. Li J. Li J.Y. Mai X.M. Zhu M.S. Cai
Using polymerase chain reaction, a 1050-bp sequence of the US1 gene was amplified from the pseudorabies virus (PRV) Becker strain genome; identification of the US1 gene was confirmed by further cloning and sequencing. Bioinformatics analysis indicated that the PRV US1 gene encodes a putative polypeptide with 349 amino acids. The encoded protein, designated P.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.22.7
- DOI:
- 10.4238/2013.January.22.7
Molecular cloning and characterization of GbDXS and GbGGPPS gene promoters from Ginkgo biloba
Authors: F. Xu X.H. Huang L.L. Li G. Deng H. Cheng X.F. Rong J.B. Li S.Y. Cheng
Ginkgolides are key pharmaceutical components in Ginkgo biloba leaves. 1-Deoxy-D-xylulose-5-phosphate synthase (GbDXS) and geranylgeranyl pyrophosphate (GbGGPPS) genes are critical genes involved in ginkgolide biosynthesis. In this study, the promoters of GbDXS and GGPPS, with 676 and 570 bp in length, respectively, were cloned by chromosome walking. The .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.3
- DOI:
- 10.4238/2013.February.4.3
Effects of grafting on key photosynthetic enzymes and gene expression in the citrus cultivar Huangguogan
Authors: L. Liao1, S.Y. Cao1, Y. Rong1 and Z.H. Wang1,2
Grafting influences scion photosynthetic capacity and fruit quality. Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which strongly affects photosynthetic rate, and Rubisco activase (RCA), which regulates Rubisco activity, are two key photosynthetic enzymes. However, little information is available regarding the effect of grafting on the concen.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017690
- DOI:
- 10.4238/gmr.15017690
Characterization of chicken natural resistance-associated macrophage protein encoding genes (Nramp1 and Nramp2) and association with salmonellosis resistance
Authors: X.M. He M.X. Fang Z.T. Zhang Y.S. Hu X.Z. Jia D.L. He S.D. Liang Q.H. Nie X.Q. Zhang
Natural resistance-associated macrophage protein 1 and 2 encoding genes (Nramp1 and Nramp2) are related to many diseases. We cloned the cDNA of chicken Nramp1 and Nramp2 genes, characterized their expression and polymorphisms, and investigated the association of some SNPs with resistance to salmonellosis. The Nramp1 cDNA was 1746 bp long and the Nramp2 cD.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.30.5
- DOI:
- 10.4238/2013.January.30.5
Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR
Authors: H.-J. Xiao, J.-H. Jin, W.-G. Chai and Z.-H. Gong
Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L... Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.10
- DOI:
- 10.4238/2015.January.23.10
Cloning and functional prediction of differentially expressed genes in the leaves of Glycine max parents and hybrids at the seedling stage
Authors: J. Zhang, D. Yao, P. Wang, S.Y. Guan, J. Ma and Y.P. Fu
Here, we compare the molecular mechanism of soybean heterosis through the differential expression of basic cloning. Specifically, we cloned 22 differentially expressed cDNA fragments from hybrid combinations of Jilin 38 x EXP (which had obvious yield advantages) and their parents. In addition, we compared the homology of these.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.February.13.15
- DOI:
- 10.4238/2014.February.13.15
Characteristics of Cyclin B and its potential role in regulating oogenesis in the red claw crayfish (Cherax quadricarinatus)
Authors: L.M. Wang, W.W. Lv, D. Zuo, Z.J. Dong and Y.L. Zhao
Cyclin B is a regulatory subunit of maturation-promoting factor (MPF), which has a key role in the induction of meiotic maturation of oocytes. MPF has been studied in a wide variety of animal species; however, its expression in crustaceans is poorly characterized. In this study, the complete cDNA sequence of Cyclin B was cloned from the red claw crayfish,.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.9.17
- DOI:
- http://dx.doi.org/10.4238/2015.September.9.17
Cloning and sequence analysis of an actin gene in aloe
Authors: S.S. Wen, D.W. He, C.M. Liao, J. Li, G.Q. Wen, and X.H. Liu
Aloe (Aloe spp), containing abundant polysaccharides and numerous bioactive ingredients, has remarkable medical, ornamental, calleidic, and edible values. In the present study, the total RNA was extracted from aloe leaf tissue. The isolated high-quality RNA was further used to clone actin gene by using reverse transcr.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.9
- DOI:
- 10.4238/2014.July.4.9