Cloning
Lipopolysaccharide and �²-1,3-glucan binding protein in the hard clam (Meretrix meretrix): Molecular characterization and expression analysis
Authors: S.X. Liu, Z.H. Qi, J.J. Zhang, C.B. He, X.G. Gao and H.J. Li
Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and β-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.4.10
- DOI:
- 10.4238/2014.July.4.10
Characterization and gene cloning of the rice (Oryza sativa L.) dwarf and narrow-leaf mutant dnl3
Authors: L. Shi, X.J. Wei, Y.M.N. Adedze, Z.H. Sheng, S.Q. Tang, P.S. Hu and J.L. Wang
The dwarf and narrow-leaf rice (Oryza sativa L.) mutant dnl3 was isolated from the Japonica cultivar Zhonghua 11 (wild-type). dnl3 exhibited pleiotropic developmental defects. The narrow-leaf phenotype resulted from a marked reduction in the number of vascular bundles, while the dwarf stature was caused by the formation of foreshortened internodes and a r.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038731
- DOI:
- 10.4238/gmr.15038731
Molecular characterization, tissue expression profile, and SNP analysis of porcine SLC13A5
Authors: L.Y. Wang, J. Jiang, H.M. Ma
Solute carrier family 13 (sodium-dependent citrate transporter member 5, SLC13A5) gene has been recently found to play an important role in intramuscular fat content in pigs. In this study, the full-length cDNA of porcine SLC13A5 was obtained from the longissimus dorsi muscle of Shaziling pigs, using the rapid amplification of.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.7.21
- DOI:
- 10.4238/2015.December.7.21
Molecular cloning and expression analysis of TRAF3 in chicken
Authors: H.L. Yang, Z.Q. Feng, S.Q. Zeng, S.M. Li, Q. Zhu and Y.P. Liu
Tumor necrosis factor receptor-associated factor 3 (TRAF3) is a crucial regulator that suppresses c-Jun N-terminal kinase and non-canonical nuclear factor-kB signaling, but facilitates type I interferon production. To determine TRAF3 function in innate immune responses among birds, particularly chicken, we cloned and characterized the chicken TRAF3 gene (.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.April.30.14
- DOI:
- 10.4238/2015.April.30.14
Cloning, expression analysis and sequence prediction of the CCAAT/enhancer-binding protein alpha gene of Qinchuan cattle
Authors: H. Wang, L.S. Zan, H.B. Wang, C. Gong and C.Z. Fu
CCAAT/enhancer-binding protein alpha (C/EBPα) is an essential transcription factor, regulating the differentiation of adipocytes. We cloned the complete open reading frame of C/EBPα gene of Qinchuan cattle and analyzed its protein structures and expression profile in 15 tissues via DNA cloning, sequencing and RT-PC.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.15.14
- DOI:
- 10.4238/2012.June.15.14
Cloning and expression analysis of cysteine protease gene (MwCP) in Agropyron mongolicum Keng
Authors: T.G.B.Y. Ao1,3, M.L. Lang2, Y.Q. Li3, Y. Zhao1, L.C. Wang1 and X.J. Yang1,2,4
In this study, a cysteine protease gene (MwCP) from Agropyron mongolicum Keng was isolated using RACE. Sequence analysis indicated that MwCP was 1473 bp, and it contained a 1134-bp open reading frame, which encoded 377 amino acids with a 24-amino acid N-terminal signal peptide. The results indicated that the MwCP protein was a new member of the papain C1A.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017424
- DOI:
- 10.4238/gmr.15017424
Expression of splice variants of cancer-testis genes ODF3 and ODF4 in the testis of a prostate cancer patient
Authors: S. Ghafouri-Fard M.H. Modarressi
The outer dense fiber (ODF) genes encode proteins that co-assemble along the axoneme of the sperm tail. Recently, it was demonstrated that some ODF genes are aberrantly expressed in tumors, including prostate adenocarcinoma, basal cell carcinoma, and chronic myeloid lymphoma. We cloned ODF3 and ODF4 cDNA from the testis of a patient suffering from prostat.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.4.11
- DOI:
- 10.4238/2012.October.4.11
Molecular characterization and tissue expression profile of porcine Ephrin-B2
Authors: D. Xu and H.M. Ma
Ephrin-B2 (EFNB2) is a signaling molecule that plays an important role in cell adhesion, proliferation, and migration in humans. However, little is known about this molecule in pigs. In order to investigate whether EFNB2 is associated with the skeletal muscle in pigs, we cloned the full-length cDNA of EFNB2 (GenBank accession No. KF500033) from the longis.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017463
- DOI:
- 10.4238/gmr.15017463
Chemical synthesis and improved expression of recombinant human granulocyte colony-stimulating factor cDNA
Authors: S. Alrokayan
Recently, granulocyte colony-stimulating factor (G-CSF) has been recognized as a useful molecule for the treatment of a wide range of complex ailments, such as cancer, AIDS, H1N1 influenza, cardiac and neurological diseases. The vast therapeutic potential of G-CSF has induced scientists to develop biotechnological approaches f.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.31.18
- DOI:
- 10.4238/2011.October.31.18
Molecular cloning, tissue expression pattern, and copy number variation of porcine SCUBE3
Authors: X. Liu*, L.G. Wang*, L.C. Zhang, H. Yan, K.B. Zhao, J. Liang, N. Li, L. Pu,T. Zhang and L.X. Wang
The signal peptide CUB EGF-like domain-containing protein 3 (SCUBE3) gene is a member of SCUBE gene family and plays important roles in bone cell biology and the determination of limb bone length. In this study, the full-length transcript of porcine SCUBE3 was cloned using reverse transcription-polymerase chain reaction and rapid amplification of cDNA end.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017010
- DOI:
- 10.4238/gmr.15017010