Cloning
Molecular cloning and expression analysis of MAT1 gene in black tiger shrimp (Penaeus monodon)
Authors: Y. Wang1,2*, M.J. Fu1,3*, C. Zhao1,3, W.Y. Bao3, F.L. Zhou1,3, Q.B. Yang1,3,4, S.G. Jiang1,3 and L.H. Qiu1,3
MAT1 (ménage à trois 1), an assembly factor and targeting subunit of the CDK-dependent kinase (CAK), can regulate the cell cycle, transcription, and DNA repair. This study was intended to investigate the role of MAT1 in the reproductive maturation of black tiger shrimp (Penaeus monodon). In this study, the P. monodon MAT1 (PmMAT1) gene was i.. Read More»
- Genet. Mol. Res. 15(1):
Identification and analysis of the jnk1 gene in polyploid hybrids of red crucian carp (Carassius auratus red var.) and common carp (Cyprinus carpio L.)
Authors: Y.M. Xiao, M.G. Jiang, Z.W. Luo, Y.H. Zhou, S. Wen, M. Wang,C. Zhang and S.J. Liu
c-Jun N-terminal kinase (JNK) is an important member of the mitogen-activated protein kinase superfamily. The allotetraploid crucian carp is a product of distant hybridization of female red crucian carp with male common carp. It is the first natural case of an allotetraploid with stable genetic characters, including fertility .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.1
- DOI:
- 10.4238/2014.February.19.1
Simple DNA extraction protocol for a 16S rDNA study of bacterial diversity in tropical landfarm soil used for bioremediation of oil waste
Authors: B.M. Maciel A.C.F. Santos J.C.T. Dias R.O. Vidal R.J.C. Dias E. Gross J.C.M. Cascardo R.P. Rezende
Landfarm soil is used to bioremediate oil wastes from petrochemical industries. We developed a simplified protocol for microbial DNA extraction of tropical landfarm soil using only direct lysis of macerated material. Two samples of tropical landfarm soil from a Brazilian refinery were analyzed by this protocol (one consisted o.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr559
- DOI:
- 10.4238/vol8-1gmr559
Homology cloning, sequence characterization, and expression analysis of cDNA encoding electron transfer flavoprotein beta polypeptide in mud crab (Scylla paramamosain)
Authors: X.L. Hao H.F. Yao Y.Z. Cheng R.X. Wang
Electron transfer flavoproteins (ETFs) are αβ-heterodimers found in eukaryotic mitochondria and bacteria. Herein we report a full-length complementary DNA of a mud crab (Scylla paramamosain) ETF β subunit (Scpa-ETFB) isolated with a homology cloning strategy. The complete complementary DNA of the Scpa-ETFB cont.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.November.12.11
- DOI:
- 10.4238/2012.November.12.11
Comparative analysis and molecular characterization of genomic sequences and proteins of FABP4 and FABP5 from the giant panda (Ailuropoda melanoleuca)
Authors: B. Song, Y.L. Hou, X. Ding, T. Wang, F. Wang, J.C. Zhong, T. Xu, J. Zhong, W.R. Hou and S.R. Shuai
Fatty acid binding proteins (FABPs) are a family of small, highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. In this study, cDNA and genomic sequences of FABP4 and FABP5 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using reverse transcription polymer.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.20.1
- DOI:
- 10.4238/2014.February.20.1
Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression
Authors: G.-F. Wu, Y.-L. Hou, W.-R. Hou, Y. Song and T. Zhang
RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The le.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr899
- DOI:
- 10.4238/vol9-4gmr899
A new approach for cloning hLIF cDNA from genomic DNA isolated from the oral mucous membrane
Authors: Y.H. Cui, G.Q. Zhu, Q.J. Chen, Y.F. Wang, M.M. Yang, Y.X. Song, J.G. Wang and B.Y. Cao
Complementary DNA (cDNA) is valuable for investigating protein structure and function in the study of life science, but it is difficult to obtain by traditional reverse transcription. We employed a novel strategy to clone human leukemia inhibitory factor (hLIF) gene cDNA from genomic DNA, which was directly isolated from the m.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.25.2
- DOI:
- 10.4238/2011.November.25.2
Cloning, partial sequence, and single-nucleotide polymorphism of the ryanodine receptor gene of the Pacific white shrimp Litopenaeus vannamei (Penaeidae)
Authors: X.H. Chen, D.G. Zeng and N. Ma
Ryanodine receptor/calcium release channel is a large protein that plays an essential role in muscle contraction; mutations in the ryanodine receptor gene affect sensitivity to stress. As a first step towards investigating the relationship between the ryanodine receptor and shrimp cramped muscle syndrome, we cloned, partially .. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr976
- DOI:
- 10.4238/vol9-4gmr976
Cloning and functional analysis of the promoter of a maize starch synthase III gene (ZmDULL1)
Authors: J.D. Wu, C.P. Jiang, H.S. Zhu, H.Y. Jiang, B.J. Cheng and S.W. Zhu
The ZmDULL1 gene encodes a starch synthase and is a determinant of the structure of endosperm starch in maize (Zea mays L.). However, little is known regarding the regulatory mechanism of the ZmDULL1 gene. In this study, we isolated and characterized the ZmDULL1 promoter (PDULL1), which is the 5' flanking region of ZmDULL1 in maize. Sequence analysis show.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.22.17
- DOI:
- 10.4238/2015.May.22.17
The noggin2 gene of Gekko japonicus (Gekkonidae) is down-regulated in the spinal cord after tail amputation
Authors: Y. Liu, Y.L. Zhou, Y.Y. Qian, Y.J. Wang, F. Ding, X.S. Gu and M. Liu
The cDNA encoding noggin2 protein was obtained from the brain and spinal cord cDNA library of Gekko japonicus. The size of the noggin2 transcript and its expression in different tissues were analyzed by Northern blot analysis. In situ hybridization revealed positive hybridization signals in both gray and white matter of the sp.. Read More»
- Genet. Mol. Res. 9(3):
- vol9-3gmr888
- DOI:
- 10.4238/vol9-3gmr888