Cloning
Molecular cloning and tissue distribution profiles of the chicken R-spondin1 gene
Authors: Y.Q. Han, J. Geng, H.T. Shi, X.M. Zhang, L.L. Du, F.T. Liu, M.M. Li, X.T. Wang, Y.Y. Wang and G.Y. Yang
Rspo1 belongs to the Rspo family, which is composed of 4 members (Rspo1-4) that share 40 to 60% sequence homology and similar domain organizations, and regulate the WNT signaling pathway via a common mechanism. Rspo1 plays a key role in vertebrate development and is an effective mitogenic factor of gastrointestinal epithelial cells. We report the cloning .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.10.19
- DOI:
- http://dx.doi.org/10.4238/2015.April.10.19
Cloning and characterization of the dehydration-responsive element-binding protein 2A gene in Eruca vesicaria subsp sativa
Authors: B.L. Huang, X.K. Zhang, Y.Y. Li, D.Y. Li, M.Y. Ma, D.T. Cai, W.H. Wu and B.Q. Huang
Eruca vesicaria subsp sativa is one of the most tolerant Cruciferae species to drought, and dehydration-responsive element-binding protein 2A (DREB2A) is involved in responses to salinity, heat, and particularly drought. In this study, a gene encoding EvDREB2A was cloned and characterized in E. vesicaria subsp sativa. The full-length EvDREB2A cDNA sequenc.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038540
- DOI:
- 10.4238/gmr.15038540
Cut-and-paste-based cloning strategy for large gene site-directed mutagenesis
Authors: C. Wang, T.Y. Wang, L.Y. Zhang, X.J. Gao, X.W. Wang and C.J. Jin
Site-directed mutagenesis is an essential technique for investigating the mechanisms of gene regulation on a molecular level, as well as for exploring post-translational modifications and functional structure at the protein level. Polymerase chain reaction combining in vitro synthesis of oligonucleotide primers allows for site-directed mutation to be perf.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.10
- DOI:
- 10.4238/2015.May.25.10
A C-type lectin fold gene from Japanese scallop Mizuhopecten yessoensis, involved with immunity and metamorphosis
Authors: X.B. Bao, C.B. He, C.D. Fu, B. Wang, X.M. Zhao, X.G. Gao andW.D. Liu
C-type lectins are a superfamily of Ca2+-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. In this study, a putative C-type lectin fold (MyCLF) gene was identified from the Japanese scallop Mizuhopecten yessoensis. The full-length of MyCLF was 645 bp, encoding a polypeptide of 16.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.11
- DOI:
- 10.4238/2015.March.27.11
Identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (PTHrP) from water buffalo (Bubalus bubalis)
Authors: J. Liu, L.D. Qian, J.L. Huo, B.L. Bi, D.L. Li, S.F. Wang,T. Chen, L.J. Li, H.M. Mao and Y.W. Miao
Parathyroid hormone-related protein (PTHrP) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. In this study, the full-length coding sequence of the water buffalo PTHrP gene was first isolated using reverse transcription-polymerase chain reaction. The protein was then subjected to molecular characterizat.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.14
- DOI:
- 10.4238/2015.March.27.14
Cloning and characterization of peanut allene oxide cyclase gene involved in salt-stressed responses
Authors: H.H. Liu, Y.G. Wang, S.P. Wang and H.J. Li
In this study, the full-length cDNA encoding allene oxide cyclase (AhAOC) was isolated from peanut (Arachis hypogaea L.). The deduced amino acid sequence of AhAOC showed high homology with other plant AOCs. The transcript of AhAOC was found to be abundantly expressed in roots. Expression analysis demonstrated that AhAOC was induced by abscisic acid, methy.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.18
- DOI:
- 10.4238/2015.March.27.18
cDNA cloning, characterization, and pharmacologic evaluation of anticancer activity of a lectin gene in Pinellia integrifolia
Authors: L.L. Liu, Z.J. Yang and Z.S. Peng
Plant lectins are proteins that possess at least one non-catalytic domain, which could reversibly bind to specific monosaccharides or oligosaccharides. The important roles played by plant lectins in immune regulation, signaling pathways, and plant defense could be attributed to their specific binding activities with carbohydrates. In this study, a Pinelli.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038516
- DOI:
- 10.4238/gmr.15038516
Wnt7b gene expression and functional analysis in the mussel Mytilus coruscus
Authors: Y.F. Xu, X. Liang, Y.R. Chen, Y.F. Li and J.L. Yang
To understand the potential functions of Wnt7b in different developmental stages and adult tissues of the mussel Mytilus coruscus, the Wnt7b gene was cloned using the rapid amplification of cDNA ends technique. The full-length Wnt7b gene was 1910 bp long, with a 1059-bp open reading frame encoding 352 amino acids. The amino ac.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048866
- DOI:
- 10.4238/gmr15048866
cDNA, genomic sequence cloning and overexpression of ribosomal protein gene L9 (rpL9) of the giant panda (Ailuropoda melanoleuca)
Authors: W.R. Hou, Y.L. Hou, G.F. Wu, Y. Song, X.L. Su, B. Sun and J. Li
The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mam.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1159
- DOI:
- 10.4238/vol10-3gmr1159
Development and significance of SCAR marker QG12-5 for Canarium album (Lour.) Raeusch by molecular cloning from improved RAPD amplification
Authors: J.L. Cheng, Z.C. Yin Z.Q. Mei, C.L. Wei, H.C. Chen, X.S. Wu and J.J. Fu
Sequence-characterized amplified region (SCAR) is a valuable molecular marker for the genetic identification of any species.This marker is mainly derived from molecular cloning of random amplified polymorphic DNA (RAPD). We have previously reported the use of an improved RAPD technique for the genetic characterization of different samples of Canarium albu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038347
- DOI:
- 10.4238/gmr.15038347