Cloning
Molecular cloning and characterization of a novel Y-box gene from Sepiella maindroni
Authors: H.P. Si, C.L.Wang, Y.Y. Zhang, C.K. Mu, P.P. Zhan, R.H. Li and W.W. Song
Y-box proteins are a family of highly conserved nucleic acid binding proteins that interact with genome and transcription product to modulate the transcriptional and translational processes. In the present study, a complete mRNA of Y-box binding protein (designated SmYB) was obtained from Sepiella maindroni by amplification of flanking sequences. The full.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.29.7
- DOI:
- 10.4238/2015.May.29.7
Toxoplasma gondii: cloning, sequencing, expression, and antigenic characterization of ROP2, GRA5 and GRA7
Authors: M. Igarashi, F. Kano, K. Tamekuni, P.M. Kawasaki, I.T. Navarro,O. Vidotto, M.C. Vidotto, R.Z. Machado and J.L. Garcia
Toxoplasma gondii is an intracellular obligate protozoan,which infects humans and warm-blooded animals. The aim of the present study was to clone the rop2, gra5 and gra7 genes from T. gondii RH strain and to produce recombinant proteins. The rop2, gra5 and gra7 gene fragments produced by polymerase chain reaction were cloned i.. Read More»
- Genet. Mol. Res. 7(2):
- vol7-2gmr423
- DOI:
- 10.4238/vol7-2gmr423
Molecular characteristics and expression of calmodulin cDNA from the freshwater pearl mussel, Hyriopsis schlegelii.
Authors: L.-G. Zeng, J.-H. Wang, Y.-J. Li, J.-Q. Sheng, Q. Gu and Y.-J. Hong
Calmodulin (CaM) is a multifunctional intracellular calcium ion receptor protein that participates in a range of cellular processes, including calcium metabolism in mussels. To investigate the role of CaM in freshwater mollusk shell calcium metabolism, the full-length CaM cDNA was isolated from the freshwater pearl mussel, Hyr.. Read More»
- Genet. Mol. Res. 11(1):
- http://dx.doi.org/2012.January.9.5
- DOI:
- http://dx.doi.org/10.4238/2012.January.9.5
Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase
Authors: N.M.A. Rahman, H.T. Fu, S.M. Sun, H. Qiao, S. Jin1,, H.K. Bai, W.Y. Zhang, G.X. Liang, Y.S. Gong, Y.W. Xiong and Y. Wu
Nitric oxide synthase (NOS) produces nitric oxide (NO) by catalyzing the conversion of l-arginine to l-citrulline, with the concomitant oxidation of nicotinamide adenine dinucleotide phosphate. Recently, various studies have verified the importance of NOS invertebrates and invertebrates. However, the NOS gene family in the ori.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038541
- DOI:
- 10.4238/gmr.15038541
Molecular cloning and characterization of the pseudorabies virus UL31 gene
Authors: M.L. Li, Z.Y. Zhao, W. Cui, C.C. Mo, J.L. Wang and M.S. Cai,
We amplified a 816-bp sequence of the UL31 gene from the pseudorabies virus (PRV) Becker strain genome. Evidence that this was the UL31 gene was confirmed by cloning and sequencing. The PRV UL31 gene encodes a putative protein of 271-amino acid residues, which was designated the UL31 protein. Bioinformatic analysis indicated t.. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.11
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.11
Molecular cloning and characterization, and prokaryotic expression of the GnRH1 gene obtained from Jinghai yellow chicken
Authors: T. Zhang, G.X. Zhang, K.P. Han, Y. Tang, J.Y. Wang, Q.C. Fan, X.S. Chen, Y. Wei and Y.J. Wang
The gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Recent studies have reported the occurrence of GnRH molecular variants in numerous species. In this study, the GnRH1 gene from Jinghai yellow chicken was cloned by reverse transcriptase-polymeras.. Read More»
- Genet. Mol. Res. 14(1):
- http://dx.doi.org/2015.March.31.14
- DOI:
- http://dx.doi.org/10.4238/2015.March.31.14
Cloning and expression analysis of heat-shock transcription factor gene CaHsfA2 from pepper (Capsicum annuum L.)
Authors: M. Guo, Y.-X. Yin, J.-J. Ji, B.-P. Ma, M.-H. Lu, and Z.-H. Gong,
The heat-shock transcription factor (Hsf) gene CaHsfA2 (GenBank accession No. JX402923) was cloned from the Capsicum annuum thermotolerant line R9 by combining the techniques electron cloning and rapid amplification of cDNA ends. The gene, which is 1436 bp in length, had an open reading frame of 1089 bp that encoded 362 amino .. Read More»
- Genet. Mol. Res. 13(1):
- http://dx.doi.org/2014.March.17.14
- DOI:
- http://dx.doi.org/10.4238/2014.March.17.14
Molecular characterization and structure analysis of RPL10/QM-like protein from the red drum Sciaenops ocellatus (Sciaenidae)
Authors: X. Chen, C.-W. Wu, S.-P. Zhong, F.-R. Zeng, J.-S. Zhang, J. Wang and S.-F. Niu
The QM-like gene encodes a ribosomal protein L10. Besides housekeeping roles in protein synthesis, QM-like proteins have multiple extraribosomal functions during cell growth, cell differentiation and apoptosis. We obtained the full-length cDNA of QM-like protein (designated as SoQM) from the salt water game fish Sciaenops ocel.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1134
- DOI:
- 10.4238/vol10-2gmr1134
Molecular cloning and tissue distribution of the Toll receptor in the black tiger shrimp, Penaeus monodon
Authors: W. Assavalapsakul and S. Panyim
The black tiger shrimp (Penaeus monodon) is economically important in many parts of the world, including Thailand. Shrimp immunity is similar to that of other invertebrate organisms; it consists of an innate immunity system. Toll or Toll-like receptors (TLRs) play an essential role in recognizing the cleaved form of the cytoki.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.6.1
- DOI:
- 10.4238/2012.March.6.1
Cloning and expression analysis of the Lonicera japonica Thunb. chlorogenic acid synthetase gene (LjCCoAOMT1) in rice
Authors: X.-H Jiang , C.-W She, Y.-H Zhu and X.-M Liu
Complete coding DNA sequences of a closely related chlorogenic acid synthetase gene (LjCCoAOMT1) were isolated from Lonicera japonica Thunb. by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). LjCCoAOMT1 was subsequently overexpressed in Escherichia coli and a 25-kD protein .. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.26.5
- DOI:
- 10.4238/2014.March.26.5