Cloning
Molecular cloning and bioinformatic analysis of the Streptococcus agalactiae neuA gene isolated from tilapia
Authors: E.L. Wang, K.Y. Wang, D.F. Chen, Y. Geng, L.Y. Huang, J. Wangand Y. He
Cytidine monophosphate (CMP) N-acetylneuraminic acid (NeuNAc) synthetase, which is encoded by the neuA gene, can catalyze the activation of sialic acid with CMP, and plays an important role in Streptococcus agalactiae infection pathogenesis. To study the structure and function of the S. agalactiae neuA gene, we isolated it from diseased tilapia, amplified.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.18
- DOI:
- 10.4238/2015.June.1.18
Identification and characterization of SREBF2 expression and its association with chicken carcass traits
Authors: F. Ye, M.H. Qiu, H.Y. Xu, X. Lan, Q. Zhu, X.L. Zhao, H.D. Yin, Y.P. Liu and Y. Wang
The sterol regulatory element-binding transcription factor 2 gene (SREBF2) plays an important role in regulating lipid homeostasis. To reveal the genetic factors that underlie carcass fat deposition in chickens, we cloned the coding DNA sequence of chicken SREBF2, investigated SREBF2 mRNA expression levels in various tissues, .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038514
- DOI:
- 10.4238/gmr.15038514
Molecular characterization of the gene checkpoint homolog 1 in Daphnia carinata during different reproductive phases
Authors: L. Kong, H.X. Li, D.L. Wu, G.R. Xu, D.L. Wang and Y.L. Zhao
Full-length cDNA of the gene checkpoint homolog 1 (Chk1) was cloned from Daphnia carinata and designated DcarChk1. DcarChk1 cDNA was 1817 bp in length and encoded a 497-amino acid polypeptide. Phylogenetic analyses revealed that DcarChk1 was most closely related to Chk1 of Daphnia pulex, followed by homologous genes of insects.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026132
- DOI:
- 10.4238/gmr.15026132
Molecular characterization of the myosatin gene and the effect of fasting on its expression in Chinese perch (Siniperca chuatsi)
Authors: L. Liu, Y.L. Li, S.D. Xu, K.Z. Wang, P. Wu, W.Y. Chu and X.Q. Wang
Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028354
- DOI:
- 10.4238/gmr.15028354
Molecular cloning and gene expression analysis of cystatin C-like proteins in spinyhead croaker Collichthys lucidus
Authors: W. Song, K.J. Jiang, F.Y. Zhang, M. Zhao and L.B. Ma
Cystatins are natural tight-binding reversible inhibitors of cysteine proteases. In this study, a cDNA library was constructed from Collichthys lucidus using the SMART technique. A complete cDNA sequence with high identity to the conserved sequence of the cystatin C gene was cloned from the library using EST analysis and rapid.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017417
- DOI:
- 10.4238/gmr.15017417
Cloning and expression of an APETALA1-like gene from Nelumbo nucifera
Authors: D.Z. Kong, X.Y. Shen, B. Guo, J.X. Dong, Y.H. Li and Y.P. Liu
The objective of this study was to clone the full-length cDNA of the APETALA1 (AP1) gene from lotus and analyze its sequence and expression pattern. The full-length cDNA sequence of the NnAP1 gene was amplified from the petals of Nelumbo nucifera ‘Hongxia’ using RT-PCR and rapid amplification of cDNA ends. Bioinformatic methods were used to an.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.18.24
- DOI:
- 10.4238/2015.June.18.24
cDNA cloning and characterization of two trehalases from Spodoptera litura (Lepidoptera; Noctuidade)
Authors: Q. Zou, P. Wei, Q. Xu, H.Z. Zheng, B. Tang and S.G. Wang
The oriental leafworm moth, Spodoptera litura, is a major agricultural pest in southeast Asia and nearby Pacific regions. Two distinct trehalases have been identified in insects: soluble trehalase (Treh1) and membrane-bound trehalase (Treh2), although there is currently no information on these genes in S. litura. To characterize the distribution and funct.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.2.7
- DOI:
- 10.4238/2013.April.2.7
Isolation and analysis of �±-expansin genes in the tree Anthocephalus chinensis (Rubiaceae)
Authors: K.X. Ouyang, M.Q. Liu, R.Q. Pian, S.S. Liu and X.Y. Chen
Expansins are cell wall-associated proteins that induce wall extension and relax stress by disrupting noncovalent bonds between cellulose microfibrils and cross-linking glycan chains, thereby promoting wall creep. Anthocephalus chinensis is a very fast-growing economically important tree found mainly in South Asia. Sixteen cDNAs, designated AcEXPA1 to AcE.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.2
- DOI:
- 10.4238/2013.April.10.2
Cloning, characterization, and expression of cadmium-induced metallothionein-2 gene from earthworm Pheretima aspergillum (E. Perrier)
Authors: L. Gong, W. Li, J. Li, W.E. Li, W.R. Wu and L.W. Yu
Metallothioneins (MTs) are ubiquitous metal-binding, cysteine-rich proteins, associated with metal accumulation and thus providing protection against toxic heavy metals such as cadmium (Cd). To investigate the mechanisms of enrichment of Cd in the earthworm Pheretima aspergillum, we isolated and cloned metallothionein-2 (MT-2) cDNA (538 bp) from P. asperg.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.5
- DOI:
- 10.4238/2015.December.14.5
Cloning and polymorphism analysis of the 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase gene (VTE3) in Arachis hypogaea, A. duranensis, and A. ipa�«nsis
Authors: F.Z. Liu, A.Q. Guo and Y.S. Wan
One of the critical enzymes involved in vitamin E biosynthesis in plants is 2-methyl-6-phytyl-1,4-benzoquinol methyltransferase (MPBQ MT). The full-length VTE3 cDNA (designated rVTE3-1 and -2) encoding MPBQ MT and the full-length DNA of VTE3 (designated gVTE3-1 and -2) were isolated from cultivated peanuts (Arachis hypogaea). The full-length DNA of VTE3 (.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.January.4.5
- DOI:
- 10.4238/2013.January.4.5