Comet Assay
Assessment of the potential genotoxic risk of medicinal Tamarindus indica fruit pulp extract using in vivo assays
Authors: F.M.V. Silva1, M.F. Leite2, A.C.C. Spadaro2, S.A. Uyemura3 andE.L. Maistro4
Tamarindus indica has been used in folk medicine as an antidiabetic, a digestive aid, and a carminative, among other uses. Currently, there is no information in the toxicology literature concerning the safety of T. indica extract. We evaluated the clastogenic and/or genotoxic potential of fruit pulp extract of this plant in vivo in peripheral blood and li.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr630
- DOI:
- 10.4238/vol8-3gmr630
Assessment of the mutagenic, genotoxic and cytotoxic potential of the waters of the Preto River in the area influenced by S?£o Jos?© do Rio Preto, SP
Authors: L.R. Maschio
-.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3ta020
- DOI:
- 10.4238/vol8-3ta020
Association of GSTM1 and GSTT1 with ageing in auto repair shop workers
Authors: S.A. Eshkoor, S.J. Marashi, P. Ismail, S.A. Rahman, M. Mirinargesi, M.Y. Adon and R.V. Devan
We evaluated the possible influence of glutathione S-transferase mu (GSTM1) and glutathione S-transferase theta (GSTT1) genes on genetic damage due to occupational exposure, which contributes to accelerate ageing. This study was conducted on 120 car auto repair workshop workers exposed to occupational hazards and 120 controls .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.21.5
- DOI:
- 10.4238/2012.May.21.5
Evaluation of pH effects on genomic integrity in adipose-derived mesenchymal stem cells using the comet assay
Authors: L.C. Hermeto, R.J. Oliveira, R. Matuo, P.H.A. Jardim, R. DeRossi, A.C.M.B. Antoniolli, E. Deffune, T.C. Evaristo and ?.E. Santana
The use of mesenchymal stem cells (MSCs) in experimental, clinical, and therapeutic trials has grown in recent years. However, the issue remains of whether these procedures are completely safe for transplant patients. Therefore, this study was designed and carried out with the aim of evaluating two different comet assay protocols for genomic damage patter.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.23.7
- DOI:
- 10.4238/2015.January.23.7
Dietary carotenoid-rich pequi oil reduces plasma lipid peroxidation and DNA damage in runners and evidence for an association with MnSOD genetic variant -Val9Ala
Authors: A.L. Miranda-Vilela, A.K. Akimoto, P.C.Z. Alves, L.C.S. Pereira,C.A. Gon?§alves, M.N. Klautau-Guimar?£es and C.K. Grisolia
Physical training induces beneficial adaptations; however, exhausting exercise increases reactive oxygen species generation, resulting in damage to DNA and tissues. Pequi (Caryocar brasiliense), a fruit of the Brazilian Cerrado, contains a carotenoid-rich oil. We investigated whether pequi oil had antioxidant effects in runner.. Read More»
- Genet. Mol. Res. 8(4):
Genotoxicity testing of Ambelania occidentalis (Apocynaceae) leaf extract in vivo
Authors: L.S. Castro, F.F. Perazzo and E.L. Maistro
Ambelania occidentalis is routinely used in folk medicine for treating gastrointestinal disorders, even though there have been no safety trials. We evaluated the genotoxic potential of hydro-alcoholic extracts of this plant in mice; induced DNA damage was assessed in peripheral blood leukocytes and micronucleus induction was a.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr588
- DOI:
- 10.4238/vol8-2gmr588
Assessment of the cytotoxic, genotoxic, and mutagenic potential of Acrocomia aculeata in rats
Authors: G.K. Traesel, L.H.A. Castro, P.V.B. Silva, R.M. Muzzi, C.A.L. Kassuya, A.C. Arena and S.A. Oesterreich
Acrocomia aculeata (Jacq.) Lodd. ex Mart. is a plant species commonly used as a foodstuff and also for treating diseases, since it contains high concentrations of antioxidant compounds and monounsaturated fatty acids. Considering its ethnopharmacological relevance, the aim of the present study was to assess the cytotoxic, genotoxic, and mutagenic effects .. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.26.13
- DOI:
- 10.4238/2015.January.26.13
Detection of damage on single- or double-stranded DNA in a population exposed to arsenic in drinking water
Authors: J. Jim?©nez-Villarreal, D.I. Rivas-Armendariz, C.P. Pineda-Belmontes, N.D. Betancourt-Mart?nez, M.A. Mac?as-Corral, A.J. Guerra-Alanis, M.S Ni?±o-Casta?±eda and J. Mor?¡n-Mart?nez
Different studies have suggested an association between arsenic (As) exposure and damage to single-stranded DNA by reactive oxygen species derived from the biotransformation of arsenic. The single strand damages are converted to double strand damage upon interaction with ultraviolet radiation. Analysis of genomic integrity is .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029241
- DOI:
- 10.4238/gmr16029241
6-Dimethylaminopurine and cyclohexamide are mutagenic and alter reproductive performance and intrauterine development in vivo
Authors: R.J. Oliveira, M.S. Mantovani, J.R. Pesarini, M.O. Mauro,A.F. da Silva, T.R. Souza and L.R. Ribeiro
The compounds 6-dimethylaminopurine (6-DMAP) and cyclohexamide (CHX) are currently used to stimulate the development of embryos produced by nuclear transfer in the production of cloned mammals with a great deal success. This study investigated the effects of 6-DMAP and CHX in vivo using biological assays to evaluate reproductive performance in females, terat.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.February.2.8
- DOI:
- 10.4238/2015.February.2.8
Antioxidant effect of haptoglobin phenotypes against DNA damage induced by hydrogen peroxide in human leukocytes
Authors: L.R.S. Moreira A.L. Miranda-Vilela I.C.R. Silva A.K. Akimoto M.N. Klautau-Guimar?£es C.K. Grisolia
Human haptoglobin is classified into three major phenotypes: Hp1-1, Hp2-1 and Hp2-2; there are two autosomal alleles Hp*1 and Hp*2, and the Hp*1 allele has two subtypes, Hp*1F and Hp*1S. Haptoglobin acts as an antioxidant, preventing hemoglobin-driven oxidative damage. We used the comet assay to examine oxidative damage to DNA.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr569
- DOI:
- 10.4238/vol8-1gmr569