Comet Assay
The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
Authors: A.C.G. Moura, F.F. Perazzo and E.L. Maistro
We examined the genotoxic and mutagenic effects of a crude extract of Clusia alata (a potential medicinal plant) on peripheral leukocyte and bone marrow cells of mice, using the comet and chromosome aberration assays. Extracts at doses of 1000, 1500 and 2000 mg/kg were administered by gavage, and a positive control, N-nitroso-N-ethylurea (50 mg/kg) was in.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr523
- DOI:
- 10.4238/vol7-4gmr523
Mutagenic potential of Cordia ecalyculata alone and in association with Spirulina maxima for their evaluation as candidate anti-obesity drugs
Authors: R.P. Araldi, B.M. Rechiutti, T.B. Mendes, E.T. Ito and E.B. Souza
Obesity is one of the most important nutritional disorders, and can be currently considered as an epidemic. Although there are few weight reduction drugs available on the market, some new drug candidates have been proposed, including Cordia ecalyculata, a Brazilian plant with anorectic properties, and Spirulina ma.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.14
- DOI:
- 10.4238/2014.July.7.14
Genotoxicity and mutagenicity of Rosmarinus officinalis (Labiatae) essential oil in mammalian cells in vivo
Authors: E.L. Maistro, S.F. Mota, E.B. Lima, B.M. Bernardes and F.C. Goulart
Rosmarinus officinalis (rosemary) oil is widely used by the cosmetic, food, and pharmaceutical industries as a fragrance component of soaps, creams, lotions, and perfumes. Although it is popular, potential harmful side-effects of the oil have been described. We investigated the genotoxic and mutagenic potential of essential oi.. Read More»
- Genet. Mol. Res. 9(4):
- vol9-4gmr857
- DOI:
- 10.4238/vol9-4gmr857
In vitro study of the cytotoxic and genotoxic effects of indomethacin-loaded Eudragit�® L 100 nanocapsules
Authors: J.G. Froder, D. Dupeyr�³n, J.C.T. Carvalho and E.L. Maistro
Indomethacin is a non-steroidal anti-inflammatory agent included in one of the most commonly used drug classes worldwide. The use of this drug results in certain side effects, including gastrointestinal complications. Therefore, there exists a need to develop better methods for the delivery of such drugs into the body, such as those employing nanoparticle.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038727
- DOI:
- 10.4238/gmr.15038727
Damaging effects of water-borne cadmium chloride on DNA of lung cells of immature mice
Authors: X.F. Yang Y.M. Ge H.T. Zhang H.M. Ning J.Q. Jiang Y.H. Qi Z.L. Wang
We investigated the effects of cadmium on lung cell DNA in immature mice. The mice were randomly divided into four groups: control group, low-dose group (1/100 LD50), middle-dose group (1/50 LD50), and high-dose group (1/25 LD50); they were supplied with cadmium chloride or control water for 40 days. Lung cells collected from sacrificed mice were used to .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.25.2
- DOI:
- 10.4238/2012.September.25.2
Mutagenicity of the Musa paradisiaca (Musaceae) fruit peel extract in mouse peripheral blood cells in vivo
Authors: C.U.B. Andrade, F.F. Perazzo and E.L. Maistro
Plants are a source of many biologically active products and nowadays they are of great interest to the pharmaceutical industry. In the present study, the mutagenic potential of the Musa paradisiaca fruit peel extract was assessed by the single-cell gel electrophoresis (SCGE) and micronucleus assays. Animals were treated orall.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3gmr462
- DOI:
- 10.4238/vol7-3gmr462
Absence of mutagenicity effects of Psidium cattleyanum Sabine (Myrtaceae) extract on peripheral blood and bone marrow cells of mice
Authors: T.D.A. Costa, S. Vieira, S.F. Andrade and E.L. Maistro
Cattley guava (Psidium cattleyanum Sabine) is a native fruit of Brazil that is popular both as a sweet food and for its reputed therapeutic properties. We examined whether it could damage DNA using the alkaline single-cell gel electrophoresis (comet assay) and the micronucleus test in leukocytes and in bone marrow.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3gmr475
- DOI:
- 10.4238/vol7-3gmr475
Genotoxic effects of rotenone on cultured lymphocytes
Authors: PatrÃ?Âcia Danielle Lima de Lima, Elizabeth Sumi Yamada, Edmar Tavares da Costa, Claudia do O. Pessoa, Silvia Helena Barem Rabenhorst, Marcelo de Oliveira Bahia, PlÃ?Ânio Cerqueira Cardoso, Raquel Alves Santos, Marilia de Arruda Cardoso Smith and Rommel RodrÃ?Âguez Burbano
Rotenone is a heterocyclic compound widely used as an insecticide, acaricide and piscicide. Its toxicity is mainly caused by the inhibition of mitochondrial respiratory processes and ATP production, resulting in the generation of reactive oxygen species. Reactive oxygen species can interact with DNA, RNA and proteins, leading .. Read More»
- Genet. Mol. Res. 4(4):
Twisting of the spermatic cord: ischemia and reperfusion, toxicogenetic evaluation, and the effects of phosphatidylcholine in pre-clinical trials
Authors: H.R.S. Coelho, C.R. Berno, G.R. Falc�£o, C.R. Hildebrand, R.J. Oliveira and A.C.M.B. Antoniolli-Silva
Phosphatidylcholine is the main phospholipid present in cell membranes and in lipoproteins, and can interfere with various biological processes. This lipid also has antioxidant activity, and protects against damage caused by free radicals under conditions of ischemia/reperfusion. Therefore, the present study was designed toeva.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038529
- DOI:
- 10.4238/gmr.15038529
Antimutagenicity and antigenotoxicity of Aloe arborescens Miller and Aloe barbadensis Miller in Aspergillus nidulans and Wistar rats
Authors: A.P. Berti, G.F. Palioto and C.L.M.S.C. Rocha
Medicinal plants such as Aloe arborescens Miller and Aloe barbadensis Miller are used by the general population to treat various diseases. Therefore, the aim of this study was to evaluate the antimutagenicity of these two species using a methG1 system in Aspergillus nidulans and the comet assay in rats. The animals were treate.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038522
- DOI:
- 10.4238/gmr.15038522