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Conservation

Human Genetics   Research Article

Inbreeding effects in Solanum lycocarpum A. St.-Hil populations, an endangered species of the Brazilian Cerrado

Authors: T.M. Moura, M.V.B.M. Siqueira, G.C.X. Oliveira

The inbreeding effective population size is an estimate of inbreeding and genetic drift in populations. It is an important tool for conservation genetics because it represents the number of individuals that are effectively contributing alleles to the subsequent generations. Several studies have been published in the last decad.. Read More»

Genet. Mol. Res. 12(4):
2013.November.26.10
DOI:
10.4238/2013.November.26.10
Plant Genetics   Research Article

Mendelian inheritance, genetic linkage, and genotypic disequilibrium at nine microsatellite loci of Cariniana legalis (Mart.) O. Kuntze

Authors: E.V. Tambarussi1, R. Vencovsky1, M.L.M. Freitas2 and A.M. Sebbenn3

Cariniana legalis is one of the largest tropical trees with a wide distribution in the Brazilian Atlantic rainforest. We investigated the Mendelian inheritance, genetic linkage, and genotypic disequilibrium at seven microsatellite loci specifically isolated for C. legalis, and at two previously developed heterologous microsatellite loci. Forty to 100 open.. Read More»

Genet. Mol. Res. 12(4):
2013.November.11.6
DOI:
10.4238/2013.November.11.6
Animal Genetics   Research Article

Polymorphic microsatellite loci isolated from Cervus unicolor (Cervidae) show inbreeding in a domesticated population of Taiwan Sambar deer

Authors: D.Y. Lin, T.Y. Chiang, C.C. Huang, H.D. Lin, S.J. Tzeng, S.R. Kang, H.M. Sung and M.C. Wu

Primers for eight microsatellites were developed; they successfully amplified DNA from 20 domesticated Formosan Sambar deer (Cervus unicolor swinhoei). All loci were polymorphic, with 10-19 alleles per locus. The average observed heterozygosity across loci and samples was 0.310, ranging from 0 to 0.750 at each locus. All loci .. Read More»

Genet. Mol. Res. 13(2):
http://dx.doi.org/2014.May.23.7
DOI:
http://dx.doi.org/10.4238/2014.May.23.7
Medical Genetics   Research Article

Using PCR-RFLP for sexing of the endangered Gal�¡pagos petrel (Pterodroma phaeopygia)

Authors: L. Pati�±o, M. Cruz, P. Mart�­nez and V. Cede�±o-Escobar

Pterodroma phaeopygia is a critically endangered avian species of the Galápagos Islands. This bird is sexually monomorphic, making it difficult to identify the sex. This information, however, is relevant to studies of behavior, ecology, and management of wild or captive populations. Here, we aimed to implement a molecular approach for determining s.. Read More»

Genet. Mol. Res. 12(4):
2013.October.18.13
DOI:
10.4238/2013.October.18.13
Animal Genetics   Research Article

Isolation and characterization of microsatellite loci in hybrid giant tiger grouper

Authors: Y.S. Huang, Z.B. Li, Y.F. Ning, J.B. Shangguan, Y. Yuan, X.Q. Mao and B.B. Li

The hybrid giant tiger grouper is a fish that has considerable commercial value and has become increasingly important for aquaculture in South East Asia since 2008. In order to prevent any reduction in genetic diversity in hybrid grouper as a result of aquaculture, we have identified 21 microsatellite markers that can be used .. Read More»

Genet. Mol. Res. 14(4):
2015.November.18.35
DOI:
10.4238/2015.November.18.35
Microbial Genetics   Research Article

Fine-scale spatial genetic structure of an endangered marsh herb, Caldesia grandis (Alismataceae)

Authors: X.-L. Yue, J.-M. Chen, Y.-H. Guo and Q.-F. Wang2

The endangered marsh herb, Caldesia grandis, is native to China. We investigated the spatial structure of the genetic variation of three populations of C. grandis using RAPD markers and spatial autocorrelation analysis, based on the method of equal distance interval. A total of 157 individuals were sampled from four patches collected from the region of Hu.. Read More»

Genet. Mol. Res. 11(3):
2012.May.18.10
DOI:
10.4238/2012.May.18.10
Microbial Genetics   Research Article

Genetic structure in fragmented populations of Solanum lycocarpum A. St.-Hil. with distinct anthropogenic histories in a Cerrado region of Brazil

Authors: T.M. Moura, K. Martins, P.S. Sujii, A.M. Sebbenn and L.J. Chaves

Solanum lycocarpum is a woody tree widely distributed in the Cerrado that reaches high population densities in disturbed environments. We examined the genetic diversity and population differentiation of six S. lycocarpum populations with different degrees of human disturbance in order to determine if they are negatively affected by anthropogenic activity... Read More»

Genet. Mol. Res. 11(3):
2012.July.10.16
DOI:
10.4238/2012.July.10.16
Animal Genetics   Research Article

Identification and characterization of polymorphic microsatellite loci in the red-crowned crane

Authors: L. Zhang, Z.H. Zhang, F.J. Shen, R. Hou, W.P. Zhang, Y.L. Liu, K.Y. Tu and A.L. Yang

We isolated and characterized microsatellite loci for the red-crowned crane (Grus japonensis) from a microsatellite-enriched database, which was obtained using high-throughput sequencing technology. We designed primer sets for 445 microsatellite loci and after initial screening, 34 loci were genotyped in 31 red-crowned cranes... Read More»

Genet. Mol. Res. 14(4):
2015.November.25.5
DOI:
10.4238/2015.November.25.5
Animal Genetics   Research Article

Genetic variability in local Brazilian horse lines using microsatellite markers

Authors: A.C.M. Silva, S.R. Paiva, M.S.M. Albuquerque, A.A. Egito, S.A. Santos, F.C. Lima, S.T. Castro, A.S. Mariante, P.S. Correa and C.M. McManus

Genetic variability at 11 microsatellite markers was analyzed in five naturalized/local Brazilian horse breeds or genetic groups. Blood samples were collected from 328 animals of the breeds Campeira (Santa Catarina State), Lavradeira (Roraima State), Pantaneira (Pantanal Mato-Grossense), Mangalarga Marchador (Minas Gerais Stat.. Read More»

Genet. Mol. Res. 11(2):
2012.April.10.4
DOI:
10.4238/2012.April.10.4
Plant Genetics   Research Article

Natural hybridization between Gossypium mustelinum and exotic allotetraploid cotton species

Authors: I.P.P. de Menezes1, J.O. da Silva1, G. Malafaia1, R.D.D. Silveira1 andP.A.V. Barroso2

Cotton has been collected in Brazil for decades for its conservation, evaluation, and the use of its genetic resources. Gossypium mustelinum is an allotetraploid cotton species that only occurs in Brazil, and little is known about its genetic potential for improvement. However, the species is threatened by habitat fragmentation and interspecific hybridiza.. Read More»

Genet. Mol. Res. 14(4):
2015.October.29.40
DOI:
10.4238/2015.October.29.40