Conservation
Genetic diversity and the conservation priority of Glycine soja populations from Northern China
Authors: Y.L. Niu, W.Y. Guo, L.R. Bai and J.C. Zhao
ABSTRACT. Knowledge of the spatial patterns of genetic variation in wild populations has significant implications for in situ conservation and the determination of conservation order. To study the levels of genetic diversity, spatial genetic structures, and genetic distances in Glycine soja, 11 natural populations in northern China were analyzed by estima.. Read More»
- Genet. Mol. Res. 14(4):
- 2015
- DOI:
- 10.4238/2015
Comparative application of direct sequencing, PCR-RFLP, and cytogenetic markers in the genetic characterization of Pimelodus (Siluriformes: Pimelodidae) species: Possible implications for fish conservation
Authors: M. Ferreira, K.C.O. Bressane, A.R.C. Moresco, O. Moreira-Filho, L.F. Almeida-Toledo and C. Garcia
Pimelodus (Pimelodidae) is a genus comprising a group of South American species with complex taxonomic relationships. Cytogenetics, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP), and sequencing data of mitochondrial genes were analyzed to characterize 4 Pimelodus species: P. fur, P. heraldoi, P... Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.17.5
- DOI:
- http://dx.doi.org/10.4238/2014.June.17.5
Genetic diversity of central and peripheral populations of Toona ciliata var. pubescens, an endangered tree species endemic to China
Authors: J. Liu, J.M. Jiang and Y.T. Chen
Our objective was to examine the genetic diversity of central and peripheral populations of Toona ciliata var. pubescens, to elucidate whether the central-peripheral hypothesis applies to these populations. We analyzed 392 individuals from nine natural populations using eight pairs of polymorphic SSR primers. The results showe.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.17.10
- DOI:
- http://dx.doi.org/10.4238/2014.June.17.10
Rapid and inexpensive analysis of genetic variability in Arapaima gigas by PCR multiplex panel of eight microsatellites
Authors: I.G. Hamoy, E.J.M. Santos and S.E.B. Santos
The aim of the present study was the development of a multiplex genotyping panel of eight microsatellite markers of Arapaima gigas, previously described. Specific primer pairs were developed, each one of them marked with either FAM-6, HEX or NED. The amplification conditions using the new primers were standardized for a single reaction. The results obtain.. Read More»
- Genet. Mol. Res. 7(1):
- vol7-1gmr394
- DOI:
- 10.4238/vol7-1gmr394
Genetic algorithm-based efficient feature selection for classification of pre-miRNAs
Authors: P. Xuan, M.Z. Guo, J. Wang, C.Y. Wang, X.Y. Liu and Y. Liu
In order to classify the real/pseudo human precursor microRNA (pre-miRNAs) hairpins with ab initio methods, numerous features are extracted from the primary sequence and second structure of pre-miRNAs. However, they include some redundant and useless features. It is essential to select the most representative feature subset; t.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr969
- DOI:
- 10.4238/vol10-2gmr969
Isolation and characterization of novel microsatellite markers in commercial selected golden Malaysian arowana fish, Scleropages formosus (Osteoglossidae)
Authors: B. Manoharan, Z. Sulaimen, F. Omar, R.Y. Othman, S.Z. Mohamed and S. Bhassu
Malaysian arowana (dragonfish; Scleropages formosus) is an ancient osteoglossid fish from southeast Asia. Due to the high demand of the ornamental fish trade and because of habitat loss, the species is close to extinction. We isolated and characterized 10 polymorphic microsatellites of this species, using 5'-anchored PCR. The .. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr944
- DOI:
- 10.4238/vol10-2gmr944
Population structure and genetic variation of the endangered species Elaeagnus mollis Diels (Elaeagnaceae)
Authors: J.M. Zhang and F. Zhang
Elaeagnus mollis Diels is a group of shrubs and dwarf trees endemic to China and are endangered plants. However, the reason why these plants are endangered remains controversial. The current study aimed to explore the endangered status of E. mollis from a genetic perspective and to propose conservation strategies for this species. Using 16 polymorphic all.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.12
- DOI:
- 10.4238/2015.June.1.12
Genetic assessment of the Atlantic Forest bristle porcupine, Chaetomys subspinosus (Rodentia: Erethizontidae), an endemic species threatened with extinction
Authors: C.G. Oliveira, R.A. Martinez, G.A.F. Gin�©, D.M. Faria and F.A. Gaiotto
The bristle-spined porcupine, Chaetomys subspinosus, an endemic rodent from Atlantic Forest, was considered to be abundant in the recent past, but population reductions due to habitat loss and expansion of human activities caused this species to be included in the “vulnerable” category of the World Conservation Uni.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1039
- DOI:
- 10.4238/vol10-2gmr1039
Isolation and characterization of 21 novel polymorphic microsatellite loci in the Chinese soft-shelled turtle Pelodiscus sinensis
Authors: X.J. Bu, L. Liu, L. Wang and L.W. Nie
Twenty-one novel polymorphic microsatellite loci were isolated from the Chinese soft-shelled turtle, Pelodiscus sinensis (Trionychidae). This is a commonly consumed Asian species, especially in China, where it is reared in large numbers on farms. We screened 34 unrelated individuals from Wuhu of Anhui province in China and det.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1119
- DOI:
- 10.4238/vol10-2gmr1119
Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers
Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang
Simple-sequence repeat (SSR) markers were employed to assess the genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China. Fourteen SSR primer pairs generated a total of 94 alleles (90 were polymorphic, accounting for 95.74%), with a mean of 6.71 alleles per locus. The number of alleles detected at each locus ranged from 2 at BPPCT .. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.7
- DOI:
- 10.4238/2015.July.28.7