Dna Isolation
Comparative analysis of genomic DNA extraction protocols: Maxi-preparation of quality DNA for genetic evaluation and phylogenetic studies
Authors: Muhammad Naeem, Shoaib Hassan, Samra Masud, Qurban Ali, Sikandar Hayat, Ammar Danyal Naeem and Zara Naeem
Four different DNA extraction protocols were compared for ability to produce DNA from the various tissues (caudal fin, pectoral fin, pelvic fin, muscle and gills) of three different freshwater fish species (Hypophthalmichthys molitrix, Labeo rohita and Sperata sarwari). Study describes the comparison of Phenol-chloroform; salt extraction.. Read More»
- Genet. Mol. Res. 19(1):
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa
Authors: V.M. Almeida, G.A. Luz, P.P. Martins, M.F.C. Gomes, M.F. Costa, P.S.C. Lima and S.E.S. Valente
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, ma.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039724
- DOI:
- 10.4238/gmr16039724
Identification of the isoamylase 3 gene in common wheat and its expression profile during the grain-filling period
Authors: G.Z. Kang, G.Q. Liu, W. Xu, Y.J. Zhu, C.Y. Wang, H.Q. Ling and T.C. Guo
In higher plants, isoamylase-type starch debranching enzyme catalyzes the α-1,6-glucosidic linkages of glycogen and phytoglycogen. We cloned an isoamylase-type starch debranching enzyme ISA3 cDNA sequence (2883 bp), designated as TaISA3, from common wheat (Triticum aestivum), using the rapid amplification of cDNA ends method. The open reading frame .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.17
- DOI:
- 10.4238/2013.February.28.17
Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.)
Authors: M. Akkurt
Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.13.8
- DOI:
- 10.4238/2012.August.13.8
An efficient protocol for tissue sampling and DNA isolation from the stem bark of Leguminosae trees
Authors: R.M.L. Novaes J.G. Rodrigues M.B. Lovato
Traditionally, molecular studies of plant species have used leaves as the source of DNA. However, sampling leaves from tall tree species can be quite difficult and expensive. We developed a sequence of procedures for using stem bark as a source of DNA from Leguminosae trees of the Atlantic Forest and the Cerrado. Leguminosae i.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr542
- DOI:
- 10.4238/vol8-1gmr542
Comparison of methods to isolate DNA from Caesalpinia ferrea
Authors: C.C. Sousa, S.O. Gomes, A.C.A. Lopes, R.L.F. Gomes, F.B. Britto, P.S.C. Lima and S.E.S. Valente
Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpini.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.16.7
- DOI:
- http://dx.doi.org/10.4238/2014.June.16.7
Improved protocol for extracting genomic DNA from frozen formalin-fixed tissue resulting in high-quality whole mtDNA
Authors: H.W. Wang, Y. Xu, H.F. Zhang, Y.J. Zeng, L. Ren, Y.L. Miao, H.Y. Luo and K.H. Wang
Formalin fixation and paraffin embedding is widely used for convenient and long-term storage of tumor tissue and precious sources to perform genetic studies. However, DNA fragmentation is one of the major flaws of genomic DNA isolation from formalin fixation tissues, which limits its further usage. Here, we present an improved method for isolating high-qu.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037972
- DOI:
- 10.4238/gmr.15037972
Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants
Authors: A.A. Alatar, M.A. Mahmoud, S.A. Al-Sohaibani and K.A. Abd-Elsalam
Medicinal plant species has a valuable economic importance because of its usage as pharmaceuticals, nutritional, as well as its use in popular medication. For DNA-based techniques, nanogram quantities of the purified DNA are requisite to amplify and yield sufficient amounts of PCR products. SDS-based DNA isolation method was u.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.13.1
- DOI:
- 10.4238/2012.February.13.1
DNA extraction from skins of wild (Hydrochoerus hydrochaeris and Pecari tajacu) and domestic (Sus scrofa domestica) species using a novel protocol
Authors: G.N. Ojeda, P.S. Amavet, E.C. Rueda and P.A. Siroski
Sometimes, commercial products obtained from wild animals are sold as if they were from domestic animals and vice versa. At this point of the productive chain, legal control of possible wildlife products is difficult. Common in the commerce of northern Argentina, skins of two wild species, the carpincho and the collared peccar.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.19.1
- DOI:
- 10.4238/2012.March.19.1
CENP-B is a conserved gene among vegetal
Authors: Olga Barbosa-Cisneros and Rafael Herrera-Esparza
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a .. Read More»
- Genet. Mol. Res. 1(3):