Dna
Myostatin (GDF8) single nucleotide polymorphisms in Nellore cattle
Authors: A.B. Grisolia1,2, G.T. D�Angelo1, L.R. Porto Neto1, F. Siqueira3 andJ.F. Garcia1
The myostatin gene, also known as GDF8 (growth differentiation factor 8), is located on bovine chromosome 2 (BTA2); it has three exons and two introns. Myostatin is specifically expressed during embryonic development and in adult skeletal muscle, functioning as a negative regulatory protein. Several cattle breeds (Piedmontese, Belgian Blue .. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr548
- DOI:
- 10.4238/vol8-3gmr548
A method for extracting period~10 modulations from DNA sequence correlations applied to the Drosophila melanogaster genome
Authors: J.C.O. Guerra P. Licinio
Nucleosome DNA packaging and positioning within the Drosophila melanogaster genome imposes a weak modulation, with a period of about 10 bp in the genomic composition correlations. We present formalism for extracting such modulations from an irreducible set of six correlation functions calculated along the D. melanogaster genome. These modulations were see.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.24.8
- DOI:
- 10.4238/2012.August.24.8
Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)
Authors: C.Y. Pang T.X. Deng D.S. Tang C.Y. Yang H. Jiang B.Z. Yang X.W. Liang
The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, inclu.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.10.3
- DOI:
- 10.4238/2012.July.10.3
Genetic instability and CpG methylation in the 5'-flanking region of the PAI-1 gene in Chinese patients with gastric cancer
Authors: J. Liu X. Li N. Yu Y.-Q. Yang X. Li Z.-Y. Ye J.-C. Li
We explored a possible correlation of genetic instability and CpG methylation in the 5'-flanking region of the PAI-1 gene with clinicopathologic features of gastric cancer in Chinese patients and looked for molecular markers for diagnosing gastric tumor development. Microsatellite instability and loss of heterozygosity of the PAI-1 gene locus D7S515, D7S471 .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.18.11
- DOI:
- 10.4238/2012.May.18.11
Genotyping of urinary samples stored with EDTA for forensic applications
Authors: S.H. Zhang S.M. Zhao Z.M. Zhao C.T. Li
Individual identification of urinary samples is necessary when sample switching or handling are suspected during a judicial process. To improve the rate of successful genotyping of urinary samples, we examined the stability of DNA in urinary samples stored for up to 30 days. Urinary samples from 20 healthy individuals (10 males and 10 females) were stored.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.May.10.5
- DOI:
- 10.4238/2012.May.10.5
Genetic diversity of mitochondrial control region (D-Loop) polymorphisms in Coilia ectenes taihuensis inhabiting Taihu Lake, China
Authors: J.J. Zhang, J.R. Duan, Y.F. Zhou, J.Y. Peng, D.A. Fang
Coilia ectenes is a commercially important fishery species in China. C. ectenes taihuensis is an endemic and dominant species found in Taihu Lake of China. When compared with C. ectenes, C. ectenes taihuensis lacks anadromous behavior, and can independently grow and reproduce in Taihu Lake. In this study, the mitochondrial DNA.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019457
- DOI:
- 10.4238/gmr16019457
cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1
Authors: W.-R. Hou Y.-L. Hou X. Ding T. Wang
The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.3.5
- DOI:
- 10.4238/2012.September.3.5
Selection pressures have driven population differentiation of domesticated and wild common carp (Cyprinus carpio L.)
Authors: L.H. Xu C.H. Wang J. Wang Z.J. Dong Y.Q. Ma X.X. Yang
Selection pressures are the principle evolutionary forces for the genetic differentiation of populations. Recent changes in selection pressures on mitochondrial DNA and microsatellite have been described in a wide variety of organisms. The common carp (Cyprinus carpio) has experienced strong selection pressure, in particular artificial selection, during i.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.12.5
- DOI:
- 10.4238/2012.September.12.5
Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds
Authors: C.S. Nascimento J.O. Peixoto L.L. Verardo C.F. Campos M.M.C. Weller V.R. Faria M.E. Botelho M.F. Martins M.A. Machado F.F. Silva P.S. Lopes S.E.F. Guimar�£es
Sacbrood disease, an affliction of honey bees (Apis mellifera) characterized by brood that fails to pupate and subsequently dies, is an important threat to honey bee health. The disease is caused by the sacbrood virus (SBV), a positive-, single-stranded RNA virus in the order Picornavirales. Because of the economic importance of honey bees for both pollinati.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.June.15.1
- DOI:
- 10.4238/2012.June.15.1
Role of mitochondrial DNA variants and copy number in diabetic atherogenesis
Authors: M.C. Chien W.T. Huang P.W. Wang C.W. Liou T.K. Lin C.J. Hsieh S.W. Weng
Hyperglycemia-induced reactive oxygen species production can cause diabetes and its complications, including atherosclerosis. The role of mitochondrial DNA variants and mitochondrial copy number in the pathogenesis of diabetic atherogenesis is not well understood. We examined 36 diabetic patients who had undergone amputation for diabetic foot and seven non-d.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.17.4
- DOI:
- 10.4238/2012.September.17.4