Dna
Cloning of a phosphatidylinositol 4-kinase gene based on fiber strength transcriptome QTL mapping in the cotton species Gossypium barbadense
Authors: H.W. Liu R.F. Shi X.F. Wang Y.X. Pan G.Y. Zang Z.Y. Ma
Sea Island cotton (Gossypium barbadense) is highly valued for its superior fiber qualities, especially fiber strength. Based on a transcript-derived fragment originated from transcriptome QTL mapping, a fiber strength related candidate gene of phosphatidylinositol 4-kinase cDNA, designated as GbPI4K, was first cloned, and its expression was characterized in .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.July.13.1
- DOI:
- 10.4238/2012.July.13.1
Prevalence of Plasmodium falciparum and P. vivax in an area of transmission located in Par?¡ State, Brazil, determined by amplification of mtDNA using a real-time PCR assay
Authors: C.R.T. Souza T.A.A. Carvalho R.C.G. Amaral L.S. Cunha M.G. Cunha J.F. Guerreiro
The need for a more sensitive and time-efficient assay for malaria has led to the development of molecular assays involving real-time PCR (qPCR), a procedure that has the potential to detect low levels of parasitemia, identify mixed infections, and allow for precise differentiation of species via melting curve analysis or TaqMan fluorescence-labeled probe.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.25.9
- DOI:
- 10.4238/2012.September.25.9
Nucleotide variation in the mitochondrial genome provides evidence for dual routes of postglacial recolonization and genetic recombination in the northeastern brook trout (Salvelinus fontinalis)
Authors: B.L. Pilgrim R.C. Perry J.L. Barron H.D. Marshall
Levels and patterns of mitochondrial DNA (mtDNA) variation were examined to investigate the population structure and possible routes of postglacial recolonization of the worldâ??s northernmost native populations of brook trout (Salvelinus fontinalis), which are found in Labrador, Canada. We analyzed the sequence diversity of a 1960-bp portion of the mitoc.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.26.2
- DOI:
- 10.4238/2012.September.26.2
Methylation-sensitive amplification polymorphism analysis of fat and muscle tissues in pigs
Authors: J.D. Ma M.Z. Li S.L. Zhou C.W. Zhou X.W. Li
DNA methylation may be involved in regulating the expression of protein-coding genes, resulting in different fat and muscle phenotypes. Using a methylation-sensitive amplified polymorphism approach, we obtained 7423 bands by selective amplification of genomic DNA from six different fat depots and two heterogeneous muscle types from Duroc/Landrace/Yorkshire c.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.26.6
- DOI:
- 10.4238/2012.September.26.6
Molecular phylogeny of Colletotrichum (Sordariomycetes: Glomerellaceae) inferred from multiple gene sequences
Authors: H. Chen, Y. Ma, W.F. Zhang, T. Ma and H.X. Wu
The genus Colletotrichum contains many important plant pathogens mainly distributed in tropical areas. Previous studies that utilized morphology or single genes have failed to resolve the phylogenetic relationships among the species. In this study, sequences of β-tubulin, 28S ribosomal DNA, and ITS region from nine species were analyzed separately an.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.27
- DOI:
- 10.4238/2015.October.28.27
Amplifiability of mitochondrial, microsatellite and amelogenin DNA loci from fecal samples of red brocket deer Mazama americana (Cetartiodactyla, Cervidae)
Authors: M.L. Oliveira J.M.B. Duarte
We tried to amplify mitochondrial, microsatellite and amelogenin loci in DNA from fecal samples of a wild Mazama americana population. Fifty-two deer fecal samples were collected from a 600-ha seasonal semideciduous forest fragment in a subtropical region of Brazil (21°20'S, 47°17'W), with the help of a detection dog; then, stored in ethanol and g.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.16.8
- DOI:
- 10.4238/2013.January.16.8
Analysis of genetic diversity in Larix gmelinii (Pinaceae) with RAPD and ISSR markers
Authors: L. Zhang H.G. Zhang X.F. Li
Dahurian larch (Larix gmelinii), a deciduous conifer, is the northernmost tree, native to eastern Siberia and nearby regions of China. We used growth traits and molecular markers to assess genetic variation in different L. gmelinii growing regions; 105 individual samples were collected from seven regions of the Qingshan Forestry Centre, Heilongjiang Province.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.12
- DOI:
- 10.4238/2013.January.24.12
Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA
Authors: J.B. Cajazeiras, L.M. Melo, E.S. Albuquerque, G. R?¡dis-Baptista, B.S. Cavada and V.J.F. Freitas
Low purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr639
- DOI:
- 10.4238/vol8-3gmr639
Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells
Authors: B. Canto-Canch?© M. Tzec-Sim?¡ J.I. V?¡zquez-Lor?a H. Espadas-?lvarez B.H. Ch?-Manzanero R. Rojas-Herrera R. Valdez-Ojeda L. Alzate-Gaviria
Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the mi.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.2
- DOI:
- 10.4238/2013.February.4.2
An alternate method for DNA and RNA extraction from clotted blood
Authors: Z. Zakaria S.H. Umi S.S. Mokhtar U. Mokhtar M.Z. Zaiharina A.T.A. Aziz B.P. Hoh
We developed an alternative method to extract DNA and RNA from clotted blood for genomic and molecular investigations. A combination of the TRIzol method and the QIAamp spin column were used to extract RNA from frozen clotted blood. Clotted blood was sonicated and then the QIAamp DNA Blood Mini Kit was used for DNA extraction. Extracted DNA and RNA were a.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.4
- DOI:
- 10.4238/2013.February.4.4