Dna
5-Aza-2�-deoxycytidine may influence the proliferation and apoptosis of cervical cancer cells via demethylation in a dose- and time-dependent manner
Authors: T.T. Yao S.M. Mo L.Y. Liu H.W. Lu M.L. Huang Z.Q. Lin
The methylation of tumor suppressor genes has been shown to be involved in many human cancers. 5-Aza-2’-deoxycytidine (5-Aza-CdR) can reactivate the expression of methylated tumor suppressor genes. In our study, 2 human cervical cancer cell lines, HeLa and SiHa, were treated with different concentrations (20, 10, 5, and 2.5 μM) of 5-Aza-CdR for 2.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.4.5
- DOI:
- 10.4238/2013.February.4.5
RAPD-based study of genetic variation and relationships among wild fig genotypes in Turkey
Authors: M. Akbulut, S. Ercisli and H. Karlidag
The fig tree (Ficus carica L.) is of significant socio-economic importance in Turkey, with 25% of the world’s fig production. Genetic variation and relationships among 14 wild-grown figs sampled from Coruh Valley in Turkey were characterized by random amplified polymorphic DNA (RAPD). Ninety-eight DNA fragments were scor.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr634
- DOI:
- 10.4238/vol8-3gmr634
Genetic diversity of Setipinna taty (Engraulidae) populations from the China Sea based on mitochondrial DNA control region sequences
Authors: H.Y. Li, T.J. Xu, Y.Z. Cheng, D.Q. Sun and R.X. Wang
The genetic diversity of Setipinna taty, which is commercially fished in the China Sea, was studied based on mitochondrial DNA control region sequences. PCR was used to amplify the control region fragment in 100 individuals of S. taty collected from Weihai (WH), Yantai (YT), Zhoushan (ZS), Xiangshan (XS), and Ninghai (NH) in C.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.9.1
- DOI:
- 10.4238/2012.May.9.1
Frequency of alterations in the MEFV gene and clinical signs in familial Mediterranean fever in Central Anatolia, Turkey
Authors: G.G. Ceylan, C. Ceylan and E. Ozturk
Familial Mediterranean fever is a recessive autoinflammatory disease that is frequent in Armenians, Jews, Arabs, and Turks. The MEFV gene is responsible for this disease. We looked for MEFV gene variations (polymorphism and mutations) in a population that resides in Central Anatolia, Turkey. DNA was extracted from peripheral b.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.7.4
- DOI:
- 10.4238/2012.May.7.4
Comparative evaluation of total RNA extraction methods in Theobroma cacao using shoot apical meristems
Authors: D.V. Silva1, S.M.J. Branco1, I.S.A. Holanda2, S. Royaert3, J.C. Motamayor4,J.P. Marelli4 and R.X. Corr�ªa5
Theobroma cacao is a species of great economic importance with its beans used for chocolate production. The tree has been a target of various molecular studies. It contains many polyphenols, which complicate the extraction of nucleic acids with the extraction protocols requiring a large amount of plant material. These issues, therefore, necessitate the op.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017364
- DOI:
- 10.4238/gmr.15017364
Common genetic risk factors of venous thromboembolism in Western and Asian populations
Authors: S.S. Huang1*, Y. Liu2*, Z.C. Jing3, X.J. Wang3 and Y.M. Mao1
Venous thromboembolism (VTE) is a multifactorial disorder involving both acquired and genetic risk factors. The common genetic factors in Western populations have been studied and reported for several decades, while studies on Asian populations are relatively scarce. Evidence suggests that the prevalence and genetic risk factors of VTE vary significantly .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017644
- DOI:
- 10.4238/gmr.15017644
Molecular identification of Echinococcus granulosus on the Tibetan Plateau using mitochondrial DNA markers
Authors: D. Hu, X. Song, N. Wang, X. Zhong, J. Wang, T. Liu, Z. Jiang, T. Dawa, X. Gu, X. Peng and G. Yang
Cystic echinococcosis (CE) is an important worldwide zoonotic disease that causes large economic losses and human suffering. Echinococcus granulosus, the causative agent of CE, exhibits different genotypes in different locations. In order to identify its genotypes and analyze its genetic structure on the Tibetan Plateau, we collected 72 hydatid cysts from.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.12
- DOI:
- 10.4238/2015.October.29.12
An assessment of genetic variability and relationships among wild-grown blackthorn (Prunus spinosa L.) plants based on RAPD markers
Authors: Y. Erturk, S. Ercisli, D. Maghradze, E. Orhan and G. Agar
Prunus spinosa, blackthorn, exists as wild populations that inhabit uncultivated uplands of Coruh Valley in the northeastern part of Turkey; the fruit is used to make preserves. We examined genetic diversity in wild-grown Prunus spinosa; 16 individual plants from wild populations of Coruh Valley were sampled and subjected to R.. Read More»
- Genet. Mol. Res. 8(4):
- vol8-4gmr641
- DOI:
- 10.4238/vol8-4gmr641
Molecular cloning and single nucleotide polymorphism analysis of IGF2a genes in the common carp (Cyprinus carpio)
Authors: S.Y. Su, Z.J. Dong, J.Q. Qu, Z.Y. Liang, J.Q. Zhang, L.X. Ma, W. Liu, P. Xu and X.H. Yuan
We studied whether two IGF2 transcripts in common carp are similar to those found in zebrafish. The full-length IGF2a cDNA contains a 5'-terminal untranslated region (UTR) of 105 bp, a 3'-terminal UTR of 1358 bp and an open reading frame of 612 bp, which encodes a 206-amino acid protein. A 6614-bp full-length IGF2a DNA molecul.. Read More»
- Genet. Mol. Res. 11(2):
- 0.4238/2012.May.15.3
- DOI:
- 0.4238/2012.May.15.3
A rapid and inexpensive method for isolation of total DNA from Trichoderma spp (Hypocreaceae)
Authors: J.C. Vazquez-Angulo, V. Mendez-Trujillo, D. GonzÃ?¡lez-Mendoza, A. Morales-Trejo, O. Grimaldo-Juarez and L. Cervantes-DÃ?Âaz
Extraction of high-quality genomic DNA for PCR amplification from filamentous fungi is difficult because of the complex cell wall and the high concentrations of polysaccharides and other secondary metabolites that bind to or co-precipitate with nucleic acids. We developed a modified sodium dodecyl sulfate/phenol protocol, with.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.15.8
- DOI:
- 10.4238/2012.May.15.8