Dna
Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress
Authors: J.K. Gan D.X. Zhang D.L. He X.Q. Zhang Z.Y. Chen Q.B. Luo
DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dr.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.March.15.1
- DOI:
- 10.4238/2013.March.15.1
Optimal clone identifier for genomic shotgun libraries: â??OC Identifier toolâ?
Authors: Cantao ME, Ferreira JE and Lemos EGM
In DNA microarray experiments, the gene fragments that are spotted on the slides are usually obtained by the synthesis of specific oligonucleotides that are able to amplify genes through PCR. Shotgun library sequences are an alternative to synthesis of primers for the study of each gene in the genome. The possibility of puttin.. Read More»
- Genet. Mol. Res. 6(4):
New set of microsatellites for Chinese tallow tree, Triadica sebifera
Authors: Y.F. Zhuang, Z.F. Wang and L.F. Wu
Chinese tallow (Triadica sebifera) is an important crop and ornamental tree. After it was introduced into the USA, it gradually became a noxious invasive tree in south-eastern America since the middle of the 1900s. Because only six microsatellites were reported previously in T. sebifera, to better understand the genetic divers.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029624
- DOI:
- 10.4238/gmr16029624
Estimation of genetic distance between 10 maize accessions with varying response to different levels of soil moisture
Authors: M. Aslam, F.S. Awan, I.A. Khan and A.I. Khan
Ten maize accessions (NC-9, A50-2, M-14, B-42, NC-3, T-7, N-48-1, B-34, USSR, and WFTMS) were studied to estimate the genetic distance on molecular level by random amplified polymorphic DNA. These accessions were selected on the basis of their variable responses against different levels of moisture. Twenty-five primers were us.. Read More»
- Genet. Mol. Res. 8(4):
- vol8-4gmr606
- DOI:
- 10.4238/vol8-4gmr606
Analysis of allopolyploidy-induced rapid genetic and epigenetic changes and their relationship in wheat
Authors: T. Qiu, Y.Z. Dong, X.M. Yu, N. Zhao and Y.F. Yang
We used the conventional and methylation-sensitive randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) analyses to assess genome-wide changes and explore the relationships between genetic and epigenetic variations among individuals of a newly synthesized allohexaploid wheat line whose genomic cons.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029303
- DOI:
- 10.4238/gmr16029303
Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb
Authors: W.H. Zheng, Y. Zhuo, L. Liang, W.Y. Ding, L.Y. Liang and X.F. Wang
Curcuma wenyujin is an important multifunctional medicinal herb in China. Currently, populations of C. wenyujin are decreasing, and wild individuals have almost disappeared from their natural habitats. Moreover, little is known regarding the molecular characteristics of this plant. In this study, we investigated the genetic diversity and variation of five.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.8.3
- DOI:
- http://dx.doi.org/10.4238/2015.September.8.3
Identification of Glomerella cingulata f. sp phaseoli recombinants by RAPD markers
Authors: O.A. Camargo Junior, E.A. Souza, M.C. Mendes-Costa, J.B. Santos, M.A. Soares
We examined the capacity of strains of Glomerella cin-gulata f. sp phaseoli fungus (Colletotrichum lindemuthianum sexual stage) to form recombinants, using random amplified polymorphic DNA (RAPD). Crosses of all possible combinations between strains 40, 42, 20, 21, 22, 23, 24, 25, and 26 were made on Petri dishes using M3 cult.. Read More»
- Genet. Mol. Res. 6(3):
Identification of Turkish and standard apple rootstocks by morphological and molecular markers
Authors: A. Koc, M. Akbulut, E. Orhan, Z. Celik, S. Bilgener and S. Ercisli
Two local (Vezir-1 and Vezir-2) and two standard (M9 and MM106) clonal apple rootstocks were compared using both morphological and molecular markers. International Union for the Protection of New Varieties of Plants criteria were used for morphological evaluation, which did not clearly separate these rootstocks. We tested 47 r.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2gmr600
- DOI:
- 10.4238/vol8-2gmr600
Expression profiles of DNA repair-related genes in rat target organs under subchronic cadmium exposure
Authors: Y.X. Lei, Q. Lu, C. Shao, C.C. He, Z.N. Lei and Y.Y. Lian
We aimed to evaluate the toxicity of long-term exposure to different cadmium (Cd) doses in rats and expression profiles of DNA repair-related genes. The model rats were exposed to different concentrations of CdCl2 for 3 months, and 5 DNA repair-related genes - hMSH2, MLH1, XRCC1, hOGG1, ERCC1 - were cloned in different tissues, including the liver, kidney.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.26.5
- DOI:
- 10.4238/2015.January.26.5
Chromosomal evolution in large pelagic oceanic apex predators, the barracudas (Sphyraenidae, Percomorpha)
Authors: R.X. Soares, M.B. Cioffi, L.A.C. Bertollo, A.T. Borges, G.W.W.F. Costa and W.F. Molina
Sphyraena (barracudas) represents the only genus of the Sphyraenidae family and includes 27 species distributed into the tropical and subtropical oceanic regions. These pelagic predators can reach large sizes and, thus, attracting significant interest from commercial and sport fishing. Evolutionary data for this fish group, as.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029644
- DOI:
- 10.4238/gmr16029644