Dna
Genetic variability in Melipona quinquefasciata (Hymenoptera, Apidae, Meliponini) from northeastern Brazil determined using the first internal transcribed spacer (ITS1)
Authors: J.O.P. Pereira, B.M. Freitas, D.M.M. Jorge, D.C. Torres,C.E.A. Soares and T.B. Grangeiro
Melipona quinquefasciata is a ground-nesting South American stingless bee whose geographic distribution was believed to comprise only the central and southern states of Brazil. We obtained partial sequences (about 500-570 bp) of first internal transcribed spacer (ITS1) nuclear ribosomal DNA from Melipona specimens putatively i.. Read More»
- Genet. Mol. Res. 8(2):
- vol8-2kerr025
- DOI:
- 10.4238/vol8-2kerr025
Molecular characterization of Anthurium genotypes by using DNA fingerprinting and SPAR markers
Authors: J.D. Souza Neto, T.C.B. Soares, L.B. Motta, P.D.S. Cabral and J.A. Silva
We characterized single primer amplification reaction (SPAR) molecular markers from 20 genotypes of Anthurium andraeanum Lind., including 3 from commercial varieties and 17 from 2 communities in the State of Espírito Santo, Brazil. Twenty-four SPAR, consisting of 7 random amplified polymorphic DNA and 17 inter-.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.2.6
- DOI:
- 10.4238/2014.July.2.6
Changes in methylation of genomic DNA from chicken immune organs in response to H5N1 influenza virus infection
Authors: Y.H. Zhang, J.L. Meng, Y. Gao, J.Y. Zhang, S.L. Niu, X.Z. Yu, Y.B. Li, Y.T. Guan, B.X. Sun and Z.H. Zhao
DNA methylation is an important epigenetic modification in eukaryotes, which plays a significant role in regulating gene expression. When the host is invaded by the influenza virus, gene expression is regulated via changes in DNA methylation levels or patterns, leading to the activation or suppression of relevant signaling pathways or networks, triggering.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15037382
- DOI:
- 10.4238/gmr.15037382
Cytochrome c oxidase subunit I barcoding of the green bee-eater (Merops orientalis)
Authors: I.A. Arif, H.A. Khan, M. Shobrak and J. Williams
DNA barcoding using mitochondrial cytochrome c oxidase subunit I (COI) is regarded as a standard method for species identification. Recent reports have also shown extended applications of COI gene analysis in phylogeny and molecular diversity studies. The bee-eaters are a group of near passerine birds in the family Meropidae. .. Read More»
- Genet. Mol. Res. 10(4):
Detection of fetal RHD pseudogene (RHD�¨) and hybrid RHD-CE-Ds from RHD-negative pregnant women with a free DNA fetal kit
Authors: T. Gunel, I. Kalelioglu, A. Gedikbasi, H. Ermis and K. Aydinli
Hemolytic disease of the newborn is a clinical condition in which maternal and paternal Rh blood group antigens are incompatible and the mother is negative for the antigen whereas the father is positive. Analysis of fetal cells recovered from maternal plasma can provide a highly sensitive prenatal diagnosis. The fetal RHD gene.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.26.1
- DOI:
- 10.4238/2011.October.26.1
An improved DNA marker technique for genetic characterization using RAMP-PCR with high-GC primers
Authors: C.L. Wei, J.L. Cheng, M.A. Khan, L.Q. Yang, S. Imani, H.C. Chen and J.J. Fu
Random amplified polymorphic DNA (RAPD) is a widely used molecular marker technique. As traditional RAPD has poor reproducibility and productivity, we previously developed an improved RAPD method (termed RAMP-PCR), which increased the reproducibility, number of bands, and efficiency of studies on polymorphism. To further develop the efficiency of this met.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038721
- DOI:
- 10.4238/gmr.15038721
Increased ROS generation and SOD activity in heteroplasmic tissues of transmitochondrial mice with A3243G mitochondrial DNA mutation
Authors: J. Li1, K. Zhou2, X. Meng1, Q. Wu1, S. Li1, Y. Liu2 and J. Wang1
The mitochondrial A3243G tRNALeu(UUR) mutation associated with a variety of mitochondrial disorders results in a severe respiratory deficiency, an increase in reactive oxygen species (ROS) production and activities of anti-oxidative enzyme in vitro. However, the phenotypic implications of this mutation have not been described in vivo. Here, mitochondria c.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr480
- DOI:
- 10.4238/vol7-4gmr480
Transcriptional profiles of emasculated flowers of black locust (Robinia pseudoacacia) determined using the cDNA-AFLP technique
Authors: J.X. Wang, P. Sun, C.Q. Yuan, L. Dai, Y. Zhang, B. Wu, C. Long, Y.H. Sun, Y. Li
Black locust (Robinia pseudoacacia) is a tree in the subfamily Faboideae, native to North America, that has been naturalized and widely planted in temperate Europe and Asia. Black locust has important ecological and economic value, but its quality needs improvement. Hybridization programs are important for black locust breedin.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.1.34
- DOI:
- 10.4238/2015.December.1.34
A set of novel microsatellite markers developed for an economically important tree, Dracontomelon duperreanum, in China
Authors: Z.F. Wang, H.L. Cao, L.F. Wu, Y. Guo, Q.M. Mei, M. Li, Y. Wang and Z.M. Wang
Dracontomelon duperreanum, the most representative species of the family Anacardiaceae, is an important multipurpose tree in China and Vietnam. However, no genetic diversity studies have been reported on this species. In this study, we identified 11 microsatellite markers for D. duperreanum by using the restriction-site-associ.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029578
- DOI:
- 10.4238/gmr16029578
Molecular DNA identification of medicinal plants used by traditional healers in Malaysia
Authors: N.A.A. Aziz, M.I. Ahmad, D.M. Naim
Plants have been used throughout human history for food and medicine. However, many plants are toxic, and cannot easily be morphologically distinguished from non-toxic plants. DNA identification solves this problem and is widely used. Nonetheless, plant DNA barcode identification faces a number of challenges, and many studies .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.7.5
- DOI:
- 10.4238/2015.December.7.5