Dna
Molecular cloning and differential expression of the glucocorticoid receptorgene in the estuarine tapertail anchovy Coilia nasus
Authors: F.K. Du, G.C. Xu, Y. Li, Z.J. Nie and P. Xu
To understand the regulation of the glucocorticoid receptor gene (Gcr) during loading and transport stress in fish, the Gcr gene of Coilia nasus was cloned. Gcr in C. nasus is expressed strongly in the liver and muscle, and less stronglyin the gills, brain, spleen, intestine, trunk kidney, and head kidney. Gcr expression in bo.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029125
- DOI:
- 10.4238/gmr16029125
Genotyping of SNPs associated with meat tenderness: comparison of two PCR-based methods
Authors: L.E. L�³pez-Rojas, L. Pati�±o-Cadavid, A. L�³pez-Herrera and J.J. Echeverri-Zuluaga
Single nucleotide polymorphisms (SNPs) carried in calpain (CAPN1), calpastatin (CAST), and leptin (LEP) genes are associated with meat tenderness. Due to the economic importance of this meat quality attribute, the development of fast, reliable, and affordable methods to identify bovine carriers of favorable alleles is of great.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029635
- DOI:
- 10.4238/gmr16029635
Optimization of DNA extraction from fresh leaf tissues of Melanoxylon brauna (Fabaceae)
Authors: D.B. Borges, M.B. Amorim, A.M. Waldschmidt, E. Mariano-Neto, C.V. Vivas and D.G. Pereira
Melanoxylon brauna (Fabaceae - Caesalpinioideae) is an endemic and valuable hardwood tree species in the Brazilian Atlantic rainforest; it is comparable to African ebony wood. We tested three protocols of DNA extraction based on the citrimonium bromide (CTAB) method and evaluated the quantity, purity and integrity of the DNA. .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.22.8
- DOI:
- 10.4238/2012.May.22.8
Diversity of endophytic fungal community associated with Piper hispidum (Piperaceae) leaves
Authors: R.C. Orlandelli, R.N. Alberto, C.J. Rubin Filho and J.A. Pamphile
Tropical and subtropical plants are rich in endophytic community diversity. Endophytes, mainly fungi and bacteria, inhabit the healthy plant tissues without causing any damage to the hosts. These fungi can be useful for biological control of pathogens and plant growth promotion. Some plants of the genus Piper are hosts of endophytic microorganisms; howeve.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.22.7
- DOI:
- 10.4238/2012.May.22.7
Detection of damage on single- or double-stranded DNA in a population exposed to arsenic in drinking water
Authors: J. JimÃ?©nez-Villarreal, D.I. Rivas-Armendariz, C.P. Pineda-Belmontes, N.D. Betancourt-MartÃ?Ânez, M.A. MacÃ?Âas-Corral, A.J. Guerra-Alanis, M.S NiÃ?±o-CastaÃ?±eda and J. MorÃ?¡n-MartÃ?Ânez
Different studies have suggested an association between arsenic (As) exposure and damage to single-stranded DNA by reactive oxygen species derived from the biotransformation of arsenic. The single strand damages are converted to double strand damage upon interaction with ultraviolet radiation. Analysis of genomic integrity is .. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029241
- DOI:
- 10.4238/gmr16029241
An improved protocol and a new grinding device for extraction of genomic DNA from microorganisms by a two-step extraction procedure
Authors: S.S. Zhang, D. Chen and Q. Lu
Current protocols to extract genomic DNA from microorganisms are still laborious, tedious and costly, especially for the species with thick cell walls. In order to improve the effectiveness of extracting DNA from microbial samples, a novel protocol, defined as two-step extraction method, along with an improved tissue-grinding .. Read More»
- Genet. Mol. Res. 11(2):
- 2012.May.21.10
- DOI:
- 10.4238/2012.May.21.10
Molecular authentication of Gynostemma pentaphyllum through development and application of random amplification polymorphic DNA sequence-characterized amplified region marker
Authors: J. Zhou,Y.S. Wu, R.Q. Zhao, J.F. Jiang, Y. Luo, C.T. Ma, J.Y. Qian
Due to the morphological similarities of aerial parts, it is difficult to distinguish Gynostemma pentaphyllum from Cayratia japonica, which is usually an adulterant of the former. To develop a reliable method for the identification and authentication of G. pentaphyllum, a combination of random amplification polymorphic DNA (RA.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.8.10
- DOI:
- 10.4238/2015.December.8.10
A novel technique for analyzing the similarity and dissimilarity of DNA sequences
Authors: Y.W. Liu and Y. Peng
li,j denotes the distance between the point (xi, yi) and the point (xj, yi) in graphical representation. By classifying li,j, i, j = 1, 2,…, N according to the number of points between (xi, yi) and (xj, yi), N - 1 types are obtained. The average and variance of every type are assembled by the novel invariant v = (a1, d1, a2, d2,…, aN, dN). C.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.28.2
- DOI:
- 10.4238/2014.January.28.2
Screening and identification of peritoneal metastasis-related genes of gastric adenocarcinoma using a cDNA microarray
Authors: F.H. Bai, N.J. Wang, J. Wang, L. Yang, F.M. Zhang, F. Yin, J. Liang, K.C. Wu and D.M. Fan
With the aim of identifying peritoneal metastasis-related genes in gastric cancer, we performed a broad analysis of differential gene expression between the parental cell line GC9811 and its highly metastatic peritoneal counterpart, cell line GC9811-P. Two fluorescent cDNA probes, labeled with Cy3 and Cy5 dyes, were prepared f.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.June.25.1
- DOI:
- 10.4238/2012.June.25.1
Comparison of extraction methods of total microbial DNA from freshwater
Authors: P. Li, S.F. Yang, B.B. Lv, K. Zhao, M.F. Lin, S. Zhou, X. Song and X.M. Tang
The demand for molecular analysis of aquatic microbial communities in freshwater has highlighted the need for efficient methods of DNA extraction. The centrifugation method and filtration-membrane method are 2 widely used methods for extracting DNA. The objective of this study was to compare the extraction efficiency of 3 methods, including the centrifugatio.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.January.30.16
- DOI:
- 10.4238/2015.January.30.16