Dna
An optimized mini-preparation method to obtain high-quality genomic DNA from mature leaves of sunflower
Authors: Li JT, Yang J, Chen DC, Zhang XL and Tang ZS
In order to investigate the mutation characteristics and to further examine the genetic variation of mutant sunflower (Helianthus annuus) obtained in plants grown from seeds exposed to space conditions, only the mature tissues such as leaf and flower could be used for DNA extraction after mutation characteristics were confirme.. Read More»
- Genet. Mol. Res. 6(4):
Genetic diversity in somatic mutants of grape (Vitis vinifera) cultivar Italia based on random amplified polymorphic DNA
Authors: S.H.Z. Maia C.A. Mangolin S.A.O. Collet M.F.P.S. Machado
Random amplified polymorphic DNA (RAPD) markers were used to detect polymorphism and to examine relationships among four table grape clones from northwestern Paraná, in southern Brazil. The 10 primers used for RAPD fingerprints generated 126 reproducible fragments, of which 63, 68, 76, and 72 were polymorphic in cultiva.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr536
- DOI:
- 10.4238/vol8-1gmr536
Genetic diversity of natural populations of Anemopaegma arvense (Bignoniaceae) in the Cerrado of S�£o Paulo State, Brazil
Authors: A.P. Batistini, M.P.C. TellesB.W. BertoniJ. da S. Coppede F.V. M�´ro, S. de C. Fran�§a, A.M.S. Pereira
Catuaba (Anemopaegma arvense), a Bignoniaceae species endemic to Cerrado regions, shows anticancer properties and is widely used as a stimulant in traditional medicine. We evaluated the genetic diversity of seven populations found in the State of São Paulo, using random amplified polymorphic DNA markers. After optimizat.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr538
- DOI:
- 10.4238/vol8-1gmr538
An improved extraction protocol for metagenomic DNA from a soil of the Brazilian Atlantic Rainforest
Authors: J.H. Amorim, T.N.S. Macena, G.V. Lacerda-Junior, R.P. Rezende, J.C.T. Dias, M. Brendel and J.C.M. Cascardo
The need for the prospecting for and identification of new biomolecules is a reality. Molecular techniques allow access to the metabolic potential of microorganisms via the isolation of DNA from environmental samples, i.e., without the application of microbial culture techniques. With its great biological diversity, the Atlantic Rainforest biome has a soi.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr509
- DOI:
- 10.4238/vol7-4gmr509
Comprehensive gene expression analysis of the DNA (cytosine-5) methyltransferase family in rice (Oryza sativa L.)
Authors: F. Ahmad, X. Huang, H.X. Lan, T. Huma, Y.M. Bao, J. Huang and H.S. Zhang
Cytosine DNA methylation is a conserved epigenetic regulatory mechanism in both plants and animals. DNA methyltransferases (DNA MTases) not only initiate (de novo) but also maintain the process of DNA methylation. Here, we characterized the genome-wide expression profiles of 10 cytosine DNA MTase genes belonging to 4 .. Read More»
- Genet. Mol. Res. 13(3):
- 2014.July.7.9
- DOI:
- 10.4238/2014.July.7.9
Application of molecular markers to detect DNA damage caused by environmental pollutants in lichen species
Authors: D. Cansaran-Duman, E. Altunkaynak, A. Aslan, �°. B�¼y�¼k and S. Aras
Pseudevernia furfuracea L. (Zopf), Peltigera praetextata (Flörke ex Sommerf.) Zopf, Lobaria pulmonaria (L.) Hoffm., and Usnea longissima Ach. lichen species were used as bioindicators to assess the genotoxicity of air pollutants. In the present study, we examined significant environmetal pollutants and investigate how changes may lead to damage in DN.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.4.23
- DOI:
- 10.4238/2015.May.4.23
Chemical synthesis and improved expression of recombinant human granulocyte colony-stimulating factor cDNA
Authors: S. Alrokayan
Recently, granulocyte colony-stimulating factor (G-CSF) has been recognized as a useful molecule for the treatment of a wide range of complex ailments, such as cancer, AIDS, H1N1 influenza, cardiac and neurological diseases. The vast therapeutic potential of G-CSF has induced scientists to develop biotechnological approaches f.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.31.18
- DOI:
- 10.4238/2011.October.31.18
Phylogenetic relationships of the Hucul horse from Romania inferred from mitochondrial D-loop variation
Authors: S.E. Georgescu, M.A. Manea, A. Dudu and M. Costache
The existence of the Hucul horse on Romanian territory has been documented from the very distant past; today Hucul is a unique breed that is part of the FAO Program for the Preservation of Animal Genetic Resources. We compared Hucul with several primitive European and Asiatic breeds in order to elucidate the origin of these ho.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.October.31.7
- DOI:
- 10.4238/2011.October.31.7
Genetic evidence for panmixia of Japanese eel (Anguilla japonica) populations in China
Authors: X.L. Gong, S.J. Ren, Z.K. Cui and L.J. Yue
The Japanese eel population has dramatically declined since the 1970s. In order to conserve this species, the background genetic structure affecting these populations should be well documented. Previous genetic studies of this species have produced seemingly conflicting results, ranging from no detectable heterogeneity to smal.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.January.31.3
- DOI:
- 10.4238/2014.January.31.3
An efficient protocol for tissue sampling and DNA isolation from the stem bark of Leguminosae trees
Authors: R.M.L. Novaes J.G. Rodrigues M.B. Lovato
Traditionally, molecular studies of plant species have used leaves as the source of DNA. However, sampling leaves from tall tree species can be quite difficult and expensive. We developed a sequence of procedures for using stem bark as a source of DNA from Leguminosae trees of the Atlantic Forest and the Cerrado. Leguminosae i.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr542
- DOI:
- 10.4238/vol8-1gmr542