Dna
Screening and identification of candidate genes essential for cervical lymph node metastasis in nasopharyngeal carcinoma with microarrays
Authors: Ming Fu, Yudong Ye, Chi Zhang, Zhuo Chen, Zhenchao Zhu, Yang Peng, Xiangning Zhang, Qianhui Qiu
The aim of the present study is to identify genes that are crucial for the cervical lymph node metastasis, which predicts poor prognosis of nasopharyngeal carcinoma (NPC), endemic in southern China. Microarrays with probes to determine genomic expression profile and promoter methylation status in tumor specimens were used, to .. Read More»
- Genet. Mol. Res. 17(1):
- gmr16039892
- DOI:
- 10.4238/gmr16039892
Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species
Authors: Asaad Gaber, Hzem Hamed, Essam Elsawy
Low-cost and low-density (LCD) DNA arrays offer an easy way to detect resistance as minimal laboratory instrumentation is needed. Nucleic acid-based amplification tests allow the rapid detection of Mycobacterium tuberculosis. Recently, a real-time PCR assay for M. tuberculosis complex was introduced. Real-Time PCR and DNA-micr.. Read More»
- Genet. Mol. Res. 16(4):
- gmr16039837
- DOI:
- 10.4238/gmr16039837
Genetics Analysis of the Biggest Cashew Tree in the World
Authors: F.P.M. do Amaral, G.H. de S�¡, L.A. Filgueiras, F.S. Santos Filho, C.J.R. Santos Soares, M.P.M. do Amaral, S.E. dos Santos Valente, A. N. Mendes
This study performed a genetic study of several canopies of Anacardium occidentale L. specimen called "Cashew King" (Cajueiro da Praia-PI, Brazil) in order to assess whether all canopies belong to the same plant. Leaves were collected from different distant points, and preserved for analysis of DNA. The analysi.. Read More»
- Genet. Mol. Res. 16(4):
- gmr16039817
- DOI:
- 10.4238/gmr16039817
DNA methylation profile of the DKK2 gene as a biomarker in patients with colorectal cancer
Authors: T.D. Silva, A.V. Felipe, V.M. Vidigal, S.S. Saad, N.M. Forones
Purpose: Epigenetic changes can be detected in precancerous lesions, suggesting that may be involved in the early stages of carcinogenesis. The methylation of specifics genes has been correlated with the outcome of many different types of cancers. This study compared the levels of DNA methylation between normal and tumor tissu.. Read More»
- Genet. Mol. Res. 16(4):
- gmr16039816
- DOI:
- 10.4238/gmr16039816
Molecular identification of variety purity in a cotton hybrid with unknown parentage using DNA-SSR markers
Authors: X.Q. Fu, F.X. Yang, X.K. Lu, X.G. Wang, B.X. Yang, F.J. Liu, Y. Liu and J. Peng
Molecular identification of hybrid purity is difficult in regional trials of cotton varieties and hybrid trials. In particular, the molecular detection of hybrid purity has not yet been reported in the case of unknown parentage. In this study, we screened 5000 pairs of primers and chose 17 pairs of core simple sequence repeat .. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039799
- DOI:
- 10.4238/gmr16039799
Genetic diversity of Bacillus sp producers of amylase isolated from the soil
Authors: A.R.E.O. Xavier, E.R. Lima, A.M.E. Oliveira, L. Cardoso, J. Santos, C.H.C. Cangussu, L.N. Leite, M.C.L. Quirino, I.G.C. J�ºnior,D.A. Oliveira and M.A.S. Xavier
The microorganisms are the best source of extracellular enzymes since they allow an economical technology with low-resource consumption compared to animals and plants. The amylases are among the most important enzymes being the genus Bacillus one of the most investigated due to its ability to produce this enzyme. The objective.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039771
- DOI:
- 10.4238/gmr16039771
Improving the PCR protocol to amplify a repetitive DNA sequence
Authors: J. Riet, L.R.V. Ramos, R.V. Lewis and L.F. Marins
Although PCR-based techniques have become an essential tool in the field of molecular and genetic research, the amplification of repetitive DNA sequences is limited. This is due to the truncated nature of the amplified sequences, which are also prone to errors during DNA polymerase-based amplification. The complex structure of repetitive DNA can form hair.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039796
- DOI:
- 10.4238/gmr16039796
Study of genetic variability in pigs after the traditional breeding program
Authors: I.M. Ferreira, G.S. Vieira, T.F. Braga, T.C.F. Silva, M.M. Franco and R.C. Antunes
Molecular markers are tools used to improve genetic gains. The objective of this study was to analyze the security of alleles of molecular marker genes for characteristics of economic interest in a pure population of pigs. After the extraction of DNA from the hair of 272 Large White matrices, the allele and genotype frequency .. Read More»
- Genet. Mol. Res. 16(3):
Full-length genomic and molecular characterization of Canine parvovirus in dogs from North of Brazil
Authors: S.P. Silva, L.N.P.P. Silva, E.D.L. Rodrigues, J.F. Cardoso, F.N. Tavares, W.M. Souza, C.M.P. Santos, F.M.S. Martins, I.S. Jesus, T.C. Brito, T.P.C. Moura, M.R.T. Nunes, L.M.N. Casseb, E. Silva Filho and A.R. Casseb
With the objective of characterizing Canine parvovirus (CPV) from some suspected fecal samples of dogs collected from the Veterinarian Hospital in Belém city, five positive samples were found by PCR assay and an update molecular characterization was provided of the CPV-2 circulation in Belém. Through sequencing o.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039719
- DOI:
- 10.4238/gmr16039719
Mutational screening in the LDLR gene among patients presenting familial hypercholesterolemia in the Southeast of Brazil
Authors: G.A. Molfetta, D.L. Zanette, J.E. Santos and W.A. Silva Jr
Familial hypercholesterolemia (FH) is a dominant, autosomal disease characterized by high LDL levels in blood plasma, and is caused by a defect in the gene encoding the LDL receptor (LDLR). The clinical diagnosis is based on personal and familial history, physical examination findings, and measures of high LDL cholesterol conc.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039226
- DOI:
- 10.4238/gmr16039226