Dna
Inflammatory response to isocyanates and onset of genomic instability in cultured human lung fibroblasts
Authors: P.K. Mishra A. Bhargava G.V. Raghuram S. Gupta S. Tiwari R. Upadhyaya S.K. Jain K.K. Maudar
Lungs comprise the primary organ exposed to environmental toxic chemicals, resulting in diverse respiratory ailments and other disorders, including carcinogenesis. Carcinogenesis is a multi-stage phenomenon, which involves a series of genetic alterations that begin with genomic instability provoked by certain factors such as i.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr544
- DOI:
- 10.4238/vol8-1gmr544
Molecular markers in commercial Bombyx mori (Lepidoptera: Bombycidae) hybrids susceptible to multiple nucleopolyhedrovirus
Authors: L.F.C. Ribeiro D.B. Zanatta J.P. Bravo R.M.C. Brancalh�£o M.A. Fernandez
The silkworm Bombyx mori L. is particularly susceptible to virus diseases, especially B. mori nucleopolyhedrovirus (BmNPV). Disease resistance, along with high productivity, are important selection criteria for developing commercial hybrids of B. mori. We used bioassays and molecular markers linked to susceptibility/resistance.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr540
- DOI:
- 10.4238/vol8-1gmr540
DNA extraction from fixed cytogenetic cell suspensions
Authors: Amorim MR, Vargas FR, Llerena Junior JC and Pombo-de-Oliveira MS
We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain r.. Read More»
- Genet. Mol. Res. 6(3):
Increase in mitochondrial DNA quantity and impairment of oxidative phosphorylation in bovine fibroblast cells treated with ethidium bromide for 15 passages in culture
Authors: Marcos Roberto Chiaratti and Fl�¡vio Vieira Meirelles
Bovine fetal fibroblast cells were treated with ethidium bromide at a low concentration for 15 passages in culture to determine its effect on mitochondrial DNA copy number and on cell metabolism. Mitochondrial membrane potential and lactate production were estimated in order to characterize cell metabolism. In addition, mitoch.. Read More»
- Genet. Mol. Res. 5(1):
Genomic DNA extraction from medicinal plants available in Malaysia using a TriOmicTM improved extraction kit
Authors: A.R. Mohd-Hairul, A.B. Sade, B.C. Yiap and A.R. Raha
DNA extraction was carried out on 32 medicinal plant samples available in Malaysia using the TriOmicTM extraction kit. Amounts of 0.1 g flowers or young leaves were ground with liquid nitrogen, lysed at 65°C in RY1plus buffer and followed by RNAse treatment. Then, RY2 buffer was added to the samples and mixed completely by.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.8.1
- DOI:
- 10.4238/2011.November.8.1
Identification of transgenic cloned dairy goats harboring human lactoferrin and methylation status of the imprinted gene IGF2R in their lungs
Authors: Y.L. Zhang, G.M. Zhang, Y.J. Wan, R.X. Jia, P.Z. Li, L. Han, F. Wang and M.R. Huang
Dairy goat is a good model for production of transgenic proteins in milk using somatic cell nuclear transfer (SCNT). However, animals produced from SCNT are often associated with lung deficiencies. We recently produced six transgenic cloned dairy goats harboring the human lactoferrin gene, including three live transgenic clones and three deceased transgen.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.22.3
- DOI:
- http://dx.doi.org/10.4238/2015.September.22.3
Expressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus
Authors: C.S. Nascimento, M.A. Machado, S.E.F. Guimar�£es, M.F. Martins, J.O. Peixoto, J. Furlong, M.C.A. Prata, R.S. Verneque, R.L. Teodoro and P.S. Lopes
Tick resistance in cattle is mainly found in zebu (Bos indicus) animals, although it is also present in some taurine (B. taurus) breeds. In order to characterize functional genes involved in tick resistance/susceptibility in cattle, two cDNA libraries were generated using skin tissues of selected Holstein x Gyr animals. A tota.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.8.3
- DOI:
- 10.4238/2011.November.8.3
Detection of polymorphisms of the mtDNA control region of Caretta caretta (Testudines: Cheloniidae) by PCR-SSCP
Authors: E.C. Reis R.M. Albano A.C.V. Bondioli L.S. Soares G. L�´bo-Hajdu
Marine turtles are increasingly being threatened worldwide by anthropogenic activities. Better understanding of their life cycle, behavior and population structure is imperative for the design of adequate conservation strategies. The mtDNA control region is a fast-evolving matrilineal marker that has been employed in the study.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr570
- DOI:
- 10.4238/vol8-1gmr570
Isolation and characterization of HC1: a novel human DNA repair gene
Authors: D.O. Lopes F.C. Falconi A.M. Goes Y. Canitrot J.S. Hoffmann C. Cazaux G.R. Franco A.M. Macedo S.D.J. Pena C.R. Machado
Nucleotide excision repair (NER) acts on a broad spectrum of large lesions, while base excision repair removes individual modified bases. Although both processes have been well studied in human cells, novel genes involved in these DNA repair pathways have been described. Using a heterologous complementation approach, we identi.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr554
- DOI:
- 10.4238/vol8-1gmr554
Population structure and demographic inferences concerning the endangered onychophoran species Epiperipatus acacioi (Onychophora: Peripatidae)
Authors: G.A. Lacorte, I.S. Oliveira and C.G. Fonseca
Epiperipatus acacioi (Onychophora: Peripatidae) is an endemic species of the Atlantic rainforest in southeastern Brazil, with a restricted known distribution, found only in two nearby areas (Tripuí and Itacolomi). Mitochondrial gene COI sequences of 93 specimens collected across the known range of E. acacioi were used t.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.9.1
- DOI:
- 10.4238/2011.November.9.1