Dna
Single primer-based DNA amplification as a suitable and low-cost tool for assessing genetic diversity in mangrove crabs
Authors: F.B. Britto, D.S.F. Mendes, M. Ogawa, I.H.A. Cintra and F.M. Diniz
We used single primer-based DNA markers to assess genetic variability of the mangrove crab, Ucides cordatus, collected from four different localities from Pará to Santa Catarina States in Brazil (almost 5000 km distant). Five primers were chosen based on the consistency of the amplified bands and the polymorphism of eac.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.9.2
- DOI:
- 10.4238/2011.November.9.2
Molecular differentiation of species of the genus Zungaro (Siluriformes, Pimelodidae) from the Amazon and Paran�¡-Paraguay River basins in Brazil
Authors: T.A. Boni, A.A. Padial, S.M.A.P. Prioli, L.C. Lucio, T.C. Maniglia, T.S. Bignotto, R.S. Panarari-Antunes, R.A. Prioli and A.J. Prioli
Fish species of the Zungaro genus (Siluriformes, Pimelodidae) are amongst the largest migratory fish in Latin America and have considerable economic importance for commercial fishing in Brazil. However, natural populations of this large catfish are experiencing a severe decline. There are significant taxonomical inconsistencie.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.10.2
- DOI:
- 10.4238/2011.November.10.2
Characterization of mitochondrial genotypes in the foundation herd of the Canchim beef cattle breed
Authors: S.C. M�©o C.R. Ferreira M.R. Chiaratti F.V. Meirelles L.C.A. Regitano M.M. Alencar P.F. Barbosa
The Canchim (5/8 Charolais + 3/8 Zebu) beef cattle breed was developed at Southeast-Embrapa Cattle to take advantage of hybrid vigor and to combine the higher growth rate and beef quality of Charolais with tropical adaptations of Zebu. The development of three lineages (old, new, and crossbred) has increased its genetic basis... Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr577
- DOI:
- 10.4238/vol8-1gmr577
Utility of DNA barcoding for rapid and accurate assessment of bat diversity in Malaysia in the absence of formally described species.
Authors: J.-J. Wilson1,2*, K.-W. Sing1*, M.R.A. Halim2, R. Ramli2,R. Hashim2 and M. Sofian-Azirun2
Bats are important flagship species for biodiversity research; however, diversity in Southeast Asia is considerably underestimated in the current checklists and field guides. Incorporation of DNA barcoding into surveys has revealed numerous species-level taxa overlooked by conventional methods. Inclusion of these taxa in inven.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.February.19.2
- DOI:
- 10.4238/2014.February.19.2
Molecular authentication of the medicinal herb Ruta graveolens (Rutaceae) and an adulterant using nuclear and chloroplast DNA markers
Authors: F. Al-Qurainy, S. Khan, M. Tarroum, F.M. Al-Hemaid and M.A. Ali
Dried parts of different plant species often look alike, especially in powdered form, making them very difficult to identify. Ruta graveolens, sold as a dried medicinal herb, can be adulterated with Euphorbia dracunculoides. The genomic DNA was isolated from the leaf powder (100 mg each) using the modified CTAB method. Interna.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.10.3
- DOI:
- 10.4238/2011.November.10.3
Comparison of methods to isolate DNA from Caesalpinia ferrea
Authors: C.C. Sousa, S.O. Gomes, A.C.A. Lopes, R.L.F. Gomes, F.B. Britto, P.S.C. Lima and S.E.S. Valente
Molecular markers are important for characterizing the genetic diversity of plants and can provide the basis for strategies to protect and conserve endangered populations. However, numerous molecular techniques are used, requiring an evaluation of fast and efficient methods to extract DNA. Since molecular studies of Caesalpini.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.16.7
- DOI:
- http://dx.doi.org/10.4238/2014.June.16.7
PedExpert: a computer program for the application of Bayesian networks to human paternity testing
Authors: R.R. Gomes S.V.A. Campos S.D.J. Pena
PedExpert is a Windows-based Bayesian network software, especially constructed to solve problems in parentage testing that are complex because of missing genetic information on the alleged father and/or because they involve genetic mutations. PedExpert automates the creation and manipulation of Bayesian networks, implementing .. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr555
- DOI:
- 10.4238/vol8-1gmr555
Multiple displacement amplification for preimplantation genetic diagnosis of fragile X syndrome
Authors: H.-S. Lee, M.J. Kim, C.K. Lim, J.W. Cho, I.O. Song and I.S. Kang
Preimplantation genetic diagnosis (PGD) has become an assisted reproductive technique for couples that have genetic risks. Despite the many advantages provided by PGD, there are several problems, including amplification failure, allele drop-out and amplification inefficiency. We evaluated multiple displacement amplification (M.. Read More»
- Genet. Mol. Res. 10(4):
- 2011.November.17.3
- DOI:
- 10.4238/2011.November.17.3
Karyotype characterization of two populations of Vernonia geminata (Asteraceae, Vernonieae) using banding and FISH techniques
Authors: V.M. Oliveira M.E. Mansanares J. Semir E.R. Forni-Martins
In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-p.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.25.1
- DOI:
- 10.4238/2012.September.25.1
Estimation of genetic diversity among sunflower genotypes through random amplified polymorphic DNA analysis
Authors: M.A. Iqbal, H.A. Sadaqat and I.A. Khan
The genetic diversity among eight sunflower lines was determined through the estimation of the random amplified polymorphic DNA method. One hundred and fifty-six DNA fragments were generated by 20 random primers, for an average of about 7.8 bands per primer. Of these amplified DNA fragments, 104 were polymorphic among the eight sunflower lines. Nei and Li.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr494
- DOI:
- 10.4238/vol7-4gmr494