Dna
Application of 16S rRNA, cytochrome b and control region sequences for understanding the phylogenetic relationships in Oryx species
Authors: H.A. Khan, I.A. Arif, A.A. Al Homaidan and A.H. Al Farhan
The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the top.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr514
- DOI:
- 10.4238/vol7-4gmr514
Optimizing reproducibility evaluation for random amplified polymorphic DNA markers
Authors: J.R. Ramos, M.P.C. Telles, J.A.F. Diniz-Filho, T.N. Soares, D.B. Melo and G. Oliveira
The random amplified polymorphic DNA (RAPD) technique is often criticized because it usually shows low levels of repeatability; thus it can generate spurious bands. These problems can be partially overcome by rigid laboratory protocols and by performing repeatability tests. However, because it is expensive and time-consuming to obtain genetic data twice f.. Read More»
- Genet. Mol. Res. 7(4):
- vol7-4gmr520
- DOI:
- 10.4238/vol7-4gmr520
Heirloom tomato gene bank: assessing genetic divergence based on morphological, agronomic and molecular data using a Ward-modified location model
Authors: L.S.A. Gon�§alves R. Rodrigues A.T. do Amaral J�ºnior M. Karasawa C.P. Sudr�©
Accessions in gene banks need to be characterized and evaluated to determine their genetic diversity. We made a joint diversity analysis of the tomato gene bank of the Universidade Estadual do Norte Fluminense Darcy Ribeiro in Rio de Janeiro state, using the Ward-modified location model. Forty Solanum lycopersicum accessions w.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr549
- DOI:
- 10.4238/vol8-1gmr549
Simple DNA extraction protocol for a 16S rDNA study of bacterial diversity in tropical landfarm soil used for bioremediation of oil waste
Authors: B.M. Maciel A.C.F. Santos J.C.T. Dias R.O. Vidal R.J.C. Dias E. Gross J.C.M. Cascardo R.P. Rezende
Landfarm soil is used to bioremediate oil wastes from petrochemical industries. We developed a simplified protocol for microbial DNA extraction of tropical landfarm soil using only direct lysis of macerated material. Two samples of tropical landfarm soil from a Brazilian refinery were analyzed by this protocol (one consisted o.. Read More»
- Genet. Mol. Res. 8(1):
- vol8-1gmr559
- DOI:
- 10.4238/vol8-1gmr559
Mitochondrial DNA haplogroups and somatic mutations are associated with lung cancer in patients from Southwest China
Authors: Y. Fang, H.Y. Yang, Y.H. Shi, J.H. Cui, L.Y. Li, Y.C. Xu and J.L. Shao
Mitochondrial DNA mutations play crucial roles in the pathogenesis and progression of human malignancies. Therefore, to determine whether maternal background or mitochondrial DNA somatic mutations were essential cofactorsin the lung cancer of Chinese patients as well, the complete mitochondrial DNA displacement loop of the primary cancerous, matched para-.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.12.6
- DOI:
- 10.4238/2015.May.12.6
Acetobacter bacteria are found in Zhenjiang vinegar grains
Authors: C.Y. Wang, J. Zhang and Z.Z. Gui
Zhenjiang vinegar, the grains of which contain a unique microbial flora, is one of the four famous traditional Chinese vinegars. We investigated the components of Zhenjiang vinegar grains. Unique acetic acid bacteria were randomly isolated from Zhenjiang vinegar grains, and the obtained strains were qualitatively analyzed to compare their capacities for a.. Read More»
- Genet. Mol. Res. 10(2):
- 2015.May.12.8
- DOI:
- 10.4238/2015.May.12.8
Cell cycle, DNA replication, repair, and recombination in the dimorphic human pathogenic fungus Paracoccidioides brasiliensis
Authors: Viviane Castelo Branco Reis, Fernando Araripe GonÃ?§alves Torres, Marcio JosÃ?© PoÃ?§as-Fonseca, Marlene Teixeira De-Souza, Diorge Paulo de Souza, JoÃ?£o Ricardo Moreira Almeida, Camila Marinho-Silva, NÃ?¡dia Skorupa Parachin, Alessandra da Silva Dantas, Thiago Machado Mello-de-Sousa and LÃ?Âdia Maria Pepe de Moraes
DNA replication, together with repair mechanisms and cell cycle control, are the most important cellular processes necessary to maintain correct transfer of genetic information to the progeny. These processes are well conserved throughout the Eukarya, and the genes that are involved provide essential information for understand.. Read More»
- Genet. Mol. Res. 4(2):
Molecular cloning, characterization, and expression analysis of a Broad-Complex homolog during development in the oriental river prawn Macrobrachium nipponense
Authors: S.F. Jiang, Y.P. Zhang, S.M. Sun, Y.S. Gong, Y.W. Xiong, H. Qiao, W.Y. Zhang, S.B. Jin and H.T. Fu
Broad-Complex (BR-C) is an early ecdysone-responsive gene encoding a family of zinc-finger transcription factors. In this study, we isolated the full-length cDNA of a BR-C homolog from the testes of the oriental river prawn (Macrobrachium nipponense), according to established expressed sequence tag information, using the rapid amplification of cDNA ends t.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.18.4
- DOI:
- 10.4238/2015.May.18.4
Cloning and characterization of the UBC gene from lotus (Nelumbo nucifera)
Authors: Y. Diao, G.L. Li, A.Q. Yu, X.W. Zheng, K.Q. Xie, Y.W. Wang, M.Q. Zhou, J. Ming and Z.L. Hu
Protein ubiquitination is extensively involved in the regulation of a considerable number of physiological processes in plant cells. E2 (ubiquitin-conjugating enzyme, UBC), one of the essential enzymes of eukaryotic ubiquitination, catalyzes protein ubiquitination together with E1 and E3. In this study, we cloned four full-length cDNANnUBCs of Nelumbo nuc.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038341
- DOI:
- 10.4238/gmr.15038341
Molecular typing of Salmonella from Sergipe, Northeastern Brazil, showing the epidemiological relationship between poultry and human infection
Authors: P.B.P. G�³is, M.R.P. Carneiro, S. Jain, M.I.S. Santos, M.V.A. Batista and A.L. C�¢ndido
Randomly amplified polymorphic DNA (RAPD) has been widely used for epidemiological and phylogenetic purposes owing to its rapidity and efficiency. The aim of this study was to perform genome typing of Salmonella samples isolated from different sources by RAPD profiling. Thirty-three Salmonella samples from the bacterial collection of the Laboratóri.. Read More»
- Genet. Mol. Res. 14(3):
- http://dx.doi.org/2015.September.25.6
- DOI:
- http://dx.doi.org/10.4238/2015.September.25.6