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Medical Genetics   Research Article

Mechanism of SEMA3B gene silencing and clinical significance in glioma

Authors: C.H. Pang, W. Du, J. Long and L.J. Song

The aim of the current study was to explore mechanisms of SEMA3B gene expression and its clinical significance in glioma, and provide a theoretical foundation for investigating individualized treatment in glioma. Paraffin-embedded tissues from 43 patients with a confirmed clinical diagnosis of glioma following neurosurgery at the First Affiliated Hospital.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017664
DOI:
10.4238/gmr.15017664
Medical Genetics   Research Article

Cytogenetic findings in patients diagnosed with breast cancer having undergone adjuvant chemotherapy regimens

Authors: P.A. Ko��ar, M. Ko�§er, D.A. ��elik and N. ��z�§elik

The aim of the present study was to investigate DNA damage in peripheral blood lymphocytes of breast cancer (BC) patients before and after administration of chemotherapy. We analyzed the frequency of sister chromatid exchange (SCE), occurrence of micronuclei (MN), and lymphocyte proliferation rate index (PRI) as cytogenetic markers in 28 female BC patient.. Read More»

Genet. Mol. Res. 15(1):
gmr.15017699
DOI:
10.4238/gmr.15017699
Plant Genetics   Research Article

Random amplified polymorphic DNA analysis of salt-tolerant tobacco mutants generated by gamma radiation

Authors: Ã?Â?. Ã?Â?elik and Ã?Â?. Atak

Salinity is one of the major problems limiting the yield of agricultural products. Radiation mutagenesis is used to improve salt-tolerant mutant plants. In this study, we aimed to improve salt-tolerant mutants of two oriental tobacco varieties. One thousand seeds of each variety (M0) were irradiated with 100, 200, 300, and 400 Gy gamma rays by Cs-137 gamm.. Read More»

Genet. Mol. Res. 14(1):
2015.February.13.12
DOI:
10.4238/2015.February.13.12
Medical Genetics   Research Article

Selection of DNA barcoding loci for Nepeta deflersiana Schweinf. ex Hedge from chloroplast and nuclear DNA genomes

Authors: F. Al-Qurainy, S. Khan, M. Nadeem, M. Tarroum and A.R.Z. Gaafar

Molecular markers, mainly DNA-based are potential tools for DNA barcoding and phylogenetic study. The plant species belonging to the Nepeta genus have important medicinal value because of the presence of nepetalactones, and they have been used to treat human diseases. We amplified nuclear and chloroplast gene loci to develop a.. Read More»

Genet. Mol. Res. 13(1):
2014.February.21.3
DOI:
10.4238/2014.February.21.3
Cytogenomics   Research Article

Genetic variation of Kaempferia (Zingiberaceae) in Thailand based on chloroplast DNA (psbA-trnH and petA-psbJ) sequences

Authors: J. Techaprasan, S. Klinbunga, C. Ngamriabsakul and T. Jenjittikul

Genetic variation and species authentication of 71 Kaempferia accessions (representing 15 recognized, six new, and four unidentified species) found indigenously in Thailand were examined by determining chloroplast psbA-trnH and partial petA-psbJ spacer sequences. Ten closely related species (Boesenbergia rotunda, Gagnepainia g.. Read More»

Genet. Mol. Res. 9(4):
vol9-4gmr873
DOI:
10.4238/vol9-4gmr873
Animal Genetics   Research Article

Giant panda ribosomal protein S14: cDNA, genomic sequence cloning, sequence analysis, and overexpression

Authors: G.-F. Wu, Y.-L. Hou, W.-R. Hou, Y. Song and T. Zhang

RPS14 is a component of the 40S ribosomal subunit encoded by the RPS14 gene and is required for its maturation. The cDNA and the genomic sequence of RPS14 were cloned successfully from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively; they were both sequenced and analyzed. The le.. Read More»

Genet. Mol. Res. 9(4):
vol9-4gmr899
DOI:
10.4238/vol9-4gmr899
Plant Genetics   Research Article

Genetic diversity and phylogenetic relationship among Tunisian cactus species (Opuntia) as revealed by random amplified microsatellite polymorphism markers

Authors: M. Bendhifi Zarroug, G. Baraket, L. Zourgui, S. Souid and A. Salhi Hannachi

Opuntia ficus indica is one of the most economically important species in the Cactaceae family. Increased interest in this crop stems from its potential contribution to agricultural diversification, application in the exploitation of marginal lands, and utility as additional income sources for farmers. In Tunisia, O. ficus indica has been affected by dras.. Read More»

Genet. Mol. Res. 14(1):
2015.February.13.21
DOI:
10.4238/2015.February.13.21
Animal Genetics   Research Article

Molecular sexing of unusually large numbers of Spheniscus magellanicus (Spheniscidae) washed ashore along the Brazilian coast in 2008

Authors: E.C. Reis, R.M. Aires, J.F. Moura, C.A.R. Matias, M. Tavares, P.H. Ott, S. Siciliano and G. L�´bo-Hajdu

There have been few studies on Magellanic penguins (Spheniscus magellanicus). In 2008, these penguins washed ashore along the Brazilian coast in unusually high numbers, some reaching as far as northeast Brazil. As Magellanic penguins show little sexual dimorphism, sex determination by morphological features is not accurate. He.. Read More»

Genet. Mol. Res. 10(4):
Plant Genetics   Research Article

Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP

Authors: R.Q. Ji, Q. Song, X.F. Xin, X. Zhou and H. Feng

To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa ssp pekinensis), differentially expressed genes in fertile and sterile plants must be isolated. We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile and sterile buds of the two-.. Read More»

Genet. Mol. Res. 10(4):
2011.December.5.8
DOI:
10.4238/2011.December.5.8
Plant Genetics   Research Article

Molecular identification of Fusarium species isolated from transgenic insect-resistant cotton plants in Mexicali valley, Baja California

Authors: T. Gonzalez-Soto, D. Gonz�¡lez-Mendoza, R. Troncoso-Rojas, A. Morales-Trejo, C. Cece�±a-Duran, A. Garcia-Lopez and O. Grimaldo-Juarez

Cotton production in the Mexicali valley is adversely affected by wilt and root rot disease associated with Fusarium species. In the present study, we sought to isolate and identify the Fusarium species in the rhizosphere of transgenic insect-resistant cotton plants grown in the Mexicali valley. Our analyses isolated four nati.. Read More»

Genet. Mol. Res. 14(4):
2015.October.2.7
DOI:
10.4238/2015.October.2.7