Dna
First record of soybean stem fly Melanagromyza sojae (Diptera: Agromyzidae) in Paraguay confirmed by molecular evidence
Authors: J.V.C. Guedes, J.A. Arnemann, L.E. Curioletti, L.M. Burtet, M.L. RamÃ?Ârez-Paredes, D. Noschang, F. Irala de Oliveira and W.T. Tay
We provided the first scientific record of Melanagromyza sojae (Zehntner, 1900), through molecular characterization of partial mtDNA COI gene, that confirms the occurrence of this pest in Paraguay. Previously reported in Brazil, an outbreak of larvae of M. sojae known as the soybean stem fly (SSF) that belongs to the family Ag.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039707
- DOI:
- 10.4238/gmr16039707
Comparison of eight methods to isolate genomic DNA from Hancornia speciosa
Authors: V.M. Almeida, G.A. Luz, P.P. Martins, M.F.C. Gomes, M.F. Costa, P.S.C. Lima and S.E.S. Valente
Mangabeira (Hancornia speciosa) is a native fruit tree found mainly in the Cerrado biome and shows great economic potential due to its multiple uses; the fruits are used in agriculture, are important as a food resource, and can be consumed in natura or processed. Due to a reduction in the area of ecosystems where it occurs, ma.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039724
- DOI:
- 10.4238/gmr16039724
Mitochondrial DNA control region analysis of three ethnic populations in lower Northern part of Thailand
Authors: U. Suyasunanont, M. Nakkuntod and S. Mirasena
The lower northern part of Thailand contains various genetically diverse ethnic populations. The sequences of the mitochondrial DNA hypervariable region were studied in three ethnic populations inhabiting Phitsanulok Province. One hundred and nine nucleotide sequences - 53, 29, and 27 from Hmongs (Hill tribe), Lao Songs, and T.. Read More»
- Genet. Mol. Res. 16(3):
- gmr16039687
- DOI:
- 10.4238/gmr16039687
Genome-wide analysis of salinity-stress induced DNA methylation alterations in cotton (Gossypium hirsutum L.) using the Me-DIP sequencing technology
Authors: X.K. Lu, N. Shu, J.J. Wang, X.G. Chen, D.L. Wang, S. Wang, W.L. Fan, X.N. Guo, L.X. Guo and W.W. Ye
Cytosine DNA methylation is a significant form of DNA modification closely associated with gene expression in eukaryotes, fungi, animals, and plants. Although the reference genomes of cotton (Gossypium hirsutum L.) have been publically available, the salinity-stress-induced DNA methylome alterations in cotton are not well unde.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029673
- DOI:
- 10.4238/gmr16029673
Genetic diversity and pectinolytic activity of epiphytic yeasts from grape carposphere
Authors: M. Ciliao Filho, M.B.D. Berteli, J.S. Valle, L.D. Paccola-Meirelles, G.A. Linde, F.G. Barcellos and N.B. Colauto
The genetic diversity of epiphytic yeasts from grape carposphere is susceptible to environmental variations that determine the predominant carposphere microbiota. Understanding the diversity of yeasts that inhabit grape carposphere in different environments and their pectinolytic activity is a way to understand the biotechnolo.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029698
- DOI:
- 10.4238/gmr16029698
Isolation, diversity, and biotechnological potential of rhizo- and endophytic bacteria associated with mangrove plants from Saudi Arabia
Authors: F. Bibi, I. Ullah, S.A. Alvi, S.A. Bakhsh, M. Yasir, A.A.K. Al-Ghamdi and E.I. Azhar
Marine bacteria have been exceptional sources of halotolerant enzymes since decades. The aim of the present study was to isolate bacteria producing hydrolytic enzymes from seven different mangroves collected from the coastal area of Thuwal, Jeddah, Saudi Arabia, and to further screen them for other enzymatic and antifungal act.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029657
- DOI:
- 10.4238/gmr16029657
SETD5 gene variant associated with mild intellectual disability - a case report
Authors: E. Stur, L.A. Soares and I.D. Louro
The recent advent of exome sequencing has allowed for the identification of pathogenic gene variants responsible for a variety of diseases that were previously clinically diagnosed, with no underlying molecular etiology. Among these conditions, intellectual disability is a prevalent heterogeneous condition, presenting itself i.. Read More»
- Genet. Mol. Res. 16(2):
- gmr16029615
- DOI:
- 10.4238/gmr16029615
MTA1 promotes cell proliferation via DNA damage repair in epithelial ovarian cancer
Authors: Q.Y. Yang1, J.H. Li2, Q.Y. Wang3, Y. Wu3, J.L. Qin3, J.J. Cheng3 and J. Qiu3
We examined whether metastasis-associated gene 1 (MTA1) promotes cell proliferation via DNA damage repair in ovarian cancer. MTA1 was successfully down-regulated using small interfering RNA in the epithelial ovarian cancer cell lines SKOV-3 and OVCAR-3. Cell growth was evaluated through MTT and colony formation assays. Fluorescence-activated cell sorting .. Read More»
- Genet. Mol. Res. 13(4):
Comparison of small scale methods for the rapid and efficient extraction of mitochondrial DNA from wheat crop suitable for down stream processes
Authors: M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu, N. Na and Z. Huiyan
We evaluated and compared 2 mitochondrial DNA (mtDNA) extraction methods in terms of DNA quality and success of subsequent polymerase chain reaction (PCR) amplifications from etiolated leaves of wheat crop (Triticum aestivum). mtDNA extraction is difficult because the presence of metabolites interfere with DNA isolation procedures and downstream applicati.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.4.27
- DOI:
- 10.4238/2014.December.4.27
Utility of hair shafts from study skins for mitochondrial DNA analysis
Authors: N. Kurihara
The condition of mtDNA in hair shafts preserved in a museum was examined using 30 study skins of masked palm civets, Paguma larvata (Viverridae), collected between 1924 and 2011. Comparisons of extracts from fresh and burnt alum-fixed hair shafts showed that burnt alum, which is commonly used in taxidermy, had no harmful effect on the amount of total DNA .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.11.1
- DOI:
- 10.4238/2013.November.11.1