Dna
Comparative study of DNA extraction methodologies from goat sperm and its effects on polymerase chain reaction analysis
Authors: E.C.B. Silva, M.A. Pelinca, A.C. Acosta, D.M.F. Silva, M.A. Gomes Filho and M.M.P. Guerra
Successful DNA extraction is indispensable for molecular methods based on polymerase chain reaction (PCR); however, goat sperm DNA extraction is limited. Thus, the aim of this study was to evaluate three methods to extract DNA from goat sperm for use in PCR. Eight goat semen pools were used for DNA extraction by using the DNea.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.21
- DOI:
- 10.4238/2014.August.7.21
Genetic characterization of natural populations of the medicinal plant Palicourea coriacea (Rubiaceae) with molecular markers
Authors: T.C.S. Barbosa, S.T. Sibov, M.P.C. Telles and T.N. Soares
Palicourea coriacea (Rubiaceae) is a herbaceous, perennial species typical of the Cerrado; it is popularly known as “douradinha”, because of its yellow flowers. It is utilized in popular medicine, mainly for the treatment of renal diseases. We used RAPD markers to evaluate the genetic structure of nine natural popu.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr671
- DOI:
- 10.4238/vol9-2gmr671
Molecular characterization and phylogenetic relationships among species of the genus Brycon (Characiformes: Characidae) from four hydrographic basins in Brazil
Authors: R.S.P. Antunes, V.N. Gomes, S.M.A.P. Prioli , R.A. Prioli, H.F. J�ºlio Jr., L.M. Prioli, C.S. Agostinho and A.J. Prioli
Brycon is one of the main genera of Neotropical freshwater fish. In Brazil, Brycon species have been found in many hydrographic basins, such as the Amazon, Paraná, Paraguay, and Araguaia-Tocantins basins. We examined the phylogenetic relationships among the species Brycon orbignyanus, B. hilarii, B. cf. pesu, B. cephalu.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr759
- DOI:
- 10.4238/vol9-2gmr759
Evaluation of attenuated Salmonella choleraesuis-mediated inhibin recombinant DNA vaccine in rats
Authors: F.M. Hui, C.L. Meng, N.N. Guo, L.G. Yang, F.X. Shi and D.G. Mao
DNA vaccination has been studied intensively as a potential vaccine technology. We evaluated the effect of an attenuated Salmonella choleraesuis-mediated inhibin DNA vaccine in rats. First, 15 rats were treated with different doses of an inhibin vaccine to evaluate vaccine safety. Next, 30 rats were divided into 3 gro.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.7.27
- DOI:
- 10.4238/2014.August.7.27
Usefulness of direct sequencing of pooled DNA for SNP identification and allele-frequency determination compatible with a common disease/common variant hypothesis
Authors: M.K. Kim, T.S. Nam, K.H. Choi, S.Y. Jang, Y.O. Kim and M.C. Lee
We examined the efficiency of direct sequencing of pooled DNA for developing common single nucleotide polymorphisms (SNPs) and its accuracy for estimating allele frequencies. A pool of 200 control DNAs was established and was used for developing SNPs and estimating minor allele frequencies (MAF). The sensitivity of the pooled .. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr761
- DOI:
- 10.4238/vol9-2gmr761
DNA repair genes in endometriosis
Authors: R. Attar, C. Cacina, S. Sozen, E. Attar and B. Agachan
Several polymorphisms in the DNA repair gene are thought to have significant effects on cancer risk. We investigated the association of polymorphisms in the DNA repair genes XRCC1 Arg399Gln, XRCC3 Thr241Met, XPD Lys751Gln, XPG Asp1104His, APE1 Asp148Glu, and HOGG1 Ser326Cys with endometriosis risk. Genotypes were determined by.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr779
- DOI:
- 10.4238/vol9-2gmr779
Establishment of the genomic in situ hybridization (GISH) technique for analysis in interspecific hybrids of Passiflora
Authors: C.A.F. Melo, G.S. Silva and M.M. Souza
The genomic in situ hybridization (GISH) technique was applied to Passiflora interspecific F1 HD13-133 hybrids (Passiflora sublanceolata x Passiflora foetida) and HD15-101 (Passiflora gardineri x Passiflora gibertii), and the backcrossed hybrids (BC1) HD18-106 and HD18-113 (Passiflora sublanceolata x HD13-133). GISH was performed using genomic probes prep.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.4
- DOI:
- 10.4238/2015.March.27.4
Characterization of a TIR-NBS-LRR gene associated with downy mildew resistance in grape
Authors: J.J. Fan, P. Wang, X. Xu, K. Liu, Y.Y. Ruan, Y.S. Zhu1, Z.H. Cui and L.J. Zhang
Grapevine downy mildew, caused by Plasmopara viticola, is a devastating disease that results in considerable economic losses as well as environmental damage through the repeated application of fungicides. The nucleotide-binding site leucine-rich repeat gene family functions in plant immunoactivity against various pathogens and pests. In this study, the 5'.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.4
- DOI:
- 10.4238/2015.July.17.4
The Caenorhabditis elegans genome: a multifractal analysis
Authors: P.E. VÃ?©lez, L.E. Garreta, E. MartÃ?Ânez, N. DÃ?Âaz, S. Amador, I. Tischer, J.M. GutiÃ?©rrez and P.A. Moreno
The Caenorhabditis elegans genome has several regular and irregular characteristics in its nucleotide composition; these are observed within and between chromosomes. To study these particularities, we carried out a multifractal analysis, which requires a large number of exponents to characterize scaling properties. We looked f.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr756
- DOI:
- 10.4238/vol9-2gmr756
A simple, fast, and inexpensive CTAB-PVP-silica based method for genomic DNA isolation from single, small insect larvae and pupae
Authors: W. Huanca-Mamani, D. Rivera-Cabello and J. Maita-Maita
In this study, we report a modified CTAB-PVP method combined with silicon dioxide (silica) treatment for the extraction of high quality genomic DNA from a single larva or pupa. This method efficiently obtains DNA from small specimens, which is difficult and challenging because of the small amount of starting tissue. Maceration with liquid nitrogen, phenol.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.17.8
- DOI:
- 10.4238/2015.July.17.8