Dna
Insights about the genetic diversity and population structure of an offshore group of common bottlenose dolphins (Tursiops truncatus) in the Mid-Atlantic
Authors: C.S. Castilho, F. Pedone-Valdez, F. Bertuol, P. Fruet, R.C. Genoves, J.C. Di Tullio, G. Caon, L.S. Hoffmann and T.R.O. Freitas
Although the genus Tursiops has a worldwide distribution and is globally well-studied, some dolphin populations continue to face high risks of decline. Hence, it is necessary to assess the genetic diversity and structure of this genus to properly assess its conservation status and to implement appropriate management actions. In Brazil, genetic studies on .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.2
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.2
Identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (PTHrP) from water buffalo (Bubalus bubalis)
Authors: J. Liu, L.D. Qian, J.L. Huo, B.L. Bi, D.L. Li, S.F. Wang,T. Chen, L.J. Li, H.M. Mao and Y.W. Miao
Parathyroid hormone-related protein (PTHrP) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. In this study, the full-length coding sequence of the water buffalo PTHrP gene was first isolated using reverse transcription-polymerase chain reaction. The protein was then subjected to molecular characterizat.. Read More»
- Genet. Mol. Res. 14(1):
- 2015.March.27.14
- DOI:
- 10.4238/2015.March.27.14
Detection of genetic polymorphism among and within Echinococcus granulosus strains by heteroduplex analysis of a microsatellite from the U1 snRNA genes
Authors: P.A. Roratto, M.L. Bartholomei-Santos, A.M. Gutierrez, L. Kamenetzky, M.C. Rosenzvit and A. Zaha
Polymerase chain reaction of a pentanucleotide microsatellite in the U1 snRNA gene complex generated a multiple band pattern due to the priming of paralogous sequences. Denaturation and slow renaturation of polymerase chain reaction products allow the formation of heteroduplex DNA that can be detected by its differential mobil.. Read More»
- Genet. Mol. Res. 5(3):
Genetic studies of â??noble caneâ? for identification and exploitation of genetic markers
Authors: S. Nawaz, F.A. Khan, S. Tabasum, M.Z. Iqbal and A. Saeed
Forty genotypes (clones) of sugarcane, including elite lines, commercial cultivars of Saccharum officinarum and clones of S. barberi were fingerprinted with 50 SSR markers using a PCR-based marker assay. Nei’s genetic distances for SSR data were determined and relationships between accessions were portrayed graphically i.. Read More»
- Genet. Mol. Res. 9(2):
- vol9-2gmr795
- DOI:
- 10.4238/vol9-2gmr795
Simulation of normal, carrier and affected controls for large-scale genotyping of cattle for factor XI deficiency P.
Authors: P.N. Mukhopadhyaya, M. Jha, P. Muraleedharan, R.R. Gupta, R.N. Rathod, H.H. Mehta and V.K. Khoda
An insertion mutation within exon 12 of the factor XI gene has been described in Holstein cattle. This has opened the prospect for large-scale screening of cattle using the polymerase chain reaction (PCR) technique for the rapid identification of heterozygous animals. To facilitate such a screening process, the mutant and norm.. Read More»
- Genet. Mol. Res. 5(2):
Increase in mitochondrial DNA quantity and impairment of oxidative phosphorylation in bovine fibroblast cells treated with ethidium bromide for 15 passages in culture
Authors: Marcos Roberto Chiaratti and Fl?¡vio Vieira Meirelles
Bovine fetal fibroblast cells were treated with ethidium bromide at a low concentration for 15 passages in culture to determine its effect on mitochondrial DNA copy number and on cell metabolism. Mitochondrial membrane potential and lactate production were estimated in order to characterize cell metabolism. In addition, mitoch.. Read More»
- Genet. Mol. Res. 5(1):
cDNA cloning, characterization, and pharmacologic evaluation of anticancer activity of a lectin gene in Pinellia integrifolia
Authors: L.L. Liu, Z.J. Yang and Z.S. Peng
Plant lectins are proteins that possess at least one non-catalytic domain, which could reversibly bind to specific monosaccharides or oligosaccharides. The important roles played by plant lectins in immune regulation, signaling pathways, and plant defense could be attributed to their specific binding activities with carbohydrates. In this study, a Pinelli.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038516
- DOI:
- 10.4238/gmr.15038516
Phylogeny of the Southwest Asian Pimpinella and related genera based on nuclear and plastid sequences
Authors: S. Fereidounfar, F. Ghahremaninejad and M. Khajehpiri
Pimpinella L. is a large genus and arguably one of the most complex genera in the family Apiaceae. In this study, the infra-generic relationship between Southwest Asian Pimpinella species and their generic allies in the tribe Pimpinelleae Spreng were investigated using sequence data from the cpDNA (chloroplast DNA) rps16 exon .. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048767
- DOI:
- 10.4238/gmr15048767
Chloroplast DNA analysis of Tunisian cork oak populations (Quercus suber L.): sequence variations and molecular evolution of the trnL (UAA)-trnF (GAA) region
Authors: A. Abdessamad, G. Baraket, H. Sakka, Y. Ammari, M. Ksontini and A. Salhi Hannachi
Sequences of the trnL-trnF spacer and combined trnL-trnF region in chloroplast DNA of cork oak (Quercus suber L.) were analyzed to detect polymorphisms and to elucidate molecular evolution and demographic history. The aligned sequences varied in length and nucleotide composition. The overall ratio of transition/transversion (t.. Read More»
- Genet. Mol. Res. 15(4):
- gmr15048749
- DOI:
- 10.4238/gmr15048749
Molecular characterization of the species Salvinia (Salviniaceae) from the upper Paran?¡ River floodplain
Authors: S.A. Machado, A.V. Oliveira, T.M.C. Fabrin, S.M.A.P. Prioli and A.J. Prioli
The pteridophytes Salvinia minima, S. herzogii, and S. auriculata are among the most abundant aquatic macrophytes in the upper Paraná River floodplain. Since some species have highly similar morphological features, it is very difficult to identify members of this genus to the species level. An indication of this difficulty is a set of poorly differ.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038575
- DOI:
- 10.4238/gmr.15038575