Dna
Inclusion of cytoplasmic lineage effect and direct-maternal genetic covariance for genetic evaluation of growth traits in Nellore cattle
Authors: L. Grigoletto, E.C. Mattos, M.H.A. Santana, F. Baldi, J.P. Eler and J.B.S. Ferraz
We evaluated the impact of cytoplasmic lineage effects (Lc) for growth traits on genetic evaluation, including the genetic covariance between direct and maternal effects (sam). Pedigree data from 496,190 Nellore animals and observations on birth weight (BW, N = 243,391), weaning weight (WW, N = 431,681), and post-weaning weight gain adjusted to 345 days (.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038812
- DOI:
- 10.4238/gmr.15038812
Comparison of methods to preserve Rheum palmatum (Polygonaceae) for efficient DNA extraction and PCR amplification
Authors: M. Huang, X.J. Sun, Y. Zhou and X.M. Wang
In this study, we compared the quality of DNA extracted using the modified CTAB method, from Rheum palmatum leaves preserved using fourteen different methods, including ones used commonly in other species: under ultra-cold (-80°C) temperatures, after drying with an absorbent paper, desiccating using a silica gel, drying at 60°C, in 70% ethanol, ab.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038019
- DOI:
- 10.4238/gmr.15038019
Development of RAPD-SCAR markers for different Ganoderma species authentication by improved RAPD amplification and molecular cloning
Authors: J.J. Fu, M.A. Khan, Z.Q. Mei, M. Tania, L.Q. Yang and J.L. Cheng
The sequence-characterized amplified region (SCAR) is a valuable molecular technique for the genetic identification of any species. This method is mainly derived from the molecular cloning of the amplified DNA fragments achieved from the random amplified polymorphic DNA (RAPD). In this study, we collected DNA from 10 species of Ganoderma mushroom and ampl.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.May.25.19
- DOI:
- 10.4238/2015.May.25.19
Isolation and characterization of 19 highly polymorphic microsatellite markers in the devil stinger, Inimicus japonicus (Synanceiidae)
Authors: X.H. Dong, M.Y. Li, X.N. Tang, X.L. Zhang and L. Miao
Inimicus japonicus, the devil stinger, has an extensive distribution along the coast of China, Japan and the Korean Peninsula. Nineteen highly polymorphic microsatellite markers were isolated and characterized in I. japonicus. Twenty-eight individuals from a wild population were tested for polymorphism using this set of polymo.. Read More»
- Genet. Mol. Res. 10(3):
DNA barcoding and phylogenetic relationships of Ardeidae (Aves: Ciconiiformes)
Authors: Z.H. Huang, M.F. Li and J.W. Qin
The avian family Ardeidae comprises long-legged freshwater and coastal birds. There has been considerable disagreement concerning the intrafamilial relationships of Ardeidae. Mitochondrial cytochrome c oxidase subunit I (COI) was used as a marker for the identification and phylogenetic analysis of avian species. In the present study, we analyzed the COI b.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038270
- DOI:
- 10.4238/gmr.15038270
Damaging effects of water-borne cadmium chloride on DNA of lung cells of immature mice
Authors: X.F. Yang Y.M. Ge H.T. Zhang H.M. Ning J.Q. Jiang Y.H. Qi Z.L. Wang
We investigated the effects of cadmium on lung cell DNA in immature mice. The mice were randomly divided into four groups: control group, low-dose group (1/100 LD50), middle-dose group (1/50 LD50), and high-dose group (1/25 LD50); they were supplied with cadmium chloride or control water for 40 days. Lung cells collected from sacrificed mice were used to .. Read More»
- Genet. Mol. Res. 11(4):
- 2012.September.25.2
- DOI:
- 10.4238/2012.September.25.2
Genetic effect of the prolactin receptor gene on egg production traits in chickens
Authors: L. Zhang D.Y. Li Y.P. Liu Y. Wang X.L. Zhao Q. Zhu
The identification and utilization of potential candidate genes with significant effects on economically important traits have become increasingly important in poultry breeding programs. The prolactin (PRLR) receptor is a specific receptor for prolactin, which is an anterior pituitary peptide hormone involved in various physiological activities and is ess.. Read More»
- Genet. Mol. Res. 11(4):
- 2012.October.2.1
- DOI:
- 10.4238/2012.October.2.1
Genetic characterization of Brazilian strains of Aspergillus flavus using DNA markers
Authors: P.P. Batista, J.F. Santos, N.T. Oliveira, A.P.D. Pires, C.M.S. Motta and E.A. Luna-Alves Lima
The Aspergillus genus belongs to a filamentous fungal group characterized by wide dispersion in the environment. Some species are associated with diseases, especially in immunocompromised patients, while others are of economical importance due to aflatoxin production or biotechnological applications. Its species identification.. Read More»
- Genet. Mol. Res. 7(3):
- vol7-3gmr422
- DOI:
- 10.4238/vol7-3gmr422
Population genetics analysis of Podocnemis sextuberculata (Testudines, Podocnemidae): lack of population structure in the central Amazon Basin
Authors: T.J. Silva, L.A.S. Monjel�³, M.N.S. Viana, J.C. Pezzuti, P.C.M. Andrade, R.C. Vogt and I.P. Farias
The chelonians are, in general, important for the economy of the traditional populations of the Amazon region, especially as a source of animal protein. Furthermore, sub-products, such as eggs and fat, are utilized in the manufacture of cosmetics, and the plastron and carapace are used in the manufacture of adornments. The fre.. Read More»
- Genet. Mol. Res. 10(3):
- vol10-3gmr1163
- DOI:
- 10.4238/vol10-3gmr1163
Association between expression of DNA mismatch repair genes and clinical features and prognosis of patients with radical resection of colon cancer
Authors: J.B. Wang, D.L. Ma, J.Y. Li, Q.D. Sun and Y.E. Liu
The aim of this study was to investigate the clinical significance of the expression of DNA mismatch repair (MMR) genes in patients subjected to radical surgical removal of colon cancer, as well as their correlation with disease prognosis. Ninety stage II and III colon cancer patients who received laparoscopic radical resection of colon cancer at our hosp.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038388
- DOI:
- 10.4238/gmr.15038388