Dna

DNATagger, colors for codons

Authors: N.M. Scherer and D.M. Basso

DNATagger is a web-based tool for coloring and editing DNA, RNA and protein sequences and alignments. It is dedicated to the visualization of protein coding sequences and also protein sequence alignments to facilitate the comprehension of evolutionary processes in sequence analysis. The distinctive feature of DNATagger is the .. Read More»

Genet. Mol. Res. 7(3):

vol7-3x-meeting003

DOI:

10.4238/vol7-3x-meeting003

Chloroplast DNA polymorphism and evolutional relationships between Asian cultivated rice (Oryza sativa) and its wild relatives (O. rufipogon)

Authors: W.J. Li B. Zhang G.W. Huang G.P. Kang M.Z. Liang L.B. Chen

We analyzed chloroplast DNA (cpDNA) polymorphism and phylogenic relationships between 6 typical indica rice, 4 japonica rice, 8 javanica rice, and 12 Asian common wild rice (Oryza rufipogon) strains collected from different latitudes in China by comparing polymorphism at 9 highly variable regions. One hundred and forty-four polymorphic bases were detected.. Read More»

Genet. Mol. Res. 11(4):

2012.October.9.8

DOI:

10.4238/2012.October.9.8

Development of two novel specific SCAR markers by cloning improved RAPD fragments from the medicinal mushroom Ganoderma lucidium (Leysser: Fr) Karst

Authors: M.A. Khan, J.L. Cheng, Z.Q. Mei, C.L. Wei and J.J. Fu

Development of sequence-characterized amplified region (SCAR) markers from random-amplified polymorphic DNA (RAPD) fragments is a valuable molecular approach for the genetic identification of different species. By using SCAR markers, molecular analysis is reduced to a simple polymerase chain reaction (PCR) analysis using primers designed from the amplicon.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038536

DOI:

10.4238/gmr.15038536

Boning up on mutations: assessing the significance of candidate disease-causing DNA sequence variation

Authors: R. Dalgleish

Genet. Mol. Res. 10(3):

vol10-3gmr1353

DOI:

10.4238/vol10-3gmr1353

Detecting the potential cancer association or metastasis by multi-omics data analysis

Authors: L. Hua, WY. Zheng, H. Xia and P. Zhou

Comprehensive multi-omics data analyses have become an important means for understanding cancer incidence and progression largely driven by the availability of high-throughput sequencing technologies for genomes, proteomes, and transcriptomes. However, how tumor cells from the site of origin of the cancer begin to grow in other sites of the body is very p.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038987

DOI:

10.4238/gmr.15038987

ChromaPipe: a pipeline for analysis, quality control and management for a DNA sequencing facility

Authors: T.D. Otto, E.A. Vasconcellos, L.H.F. Gomes, A.S. Moreira,W.M. Degrave, L. Mendon�§a-Lima and M. Alves-Ferreira

Optimizing and monitoring the data flow in high-throughput sequencing facilities is important for data input and output, for tracking the status of results for the users of the facility, and to guarantee a good, high-quality service. In a multi-user system environment with different throughputs, each user wants to access his/h.. Read More»

Genet. Mol. Res. 7(3):

vol7-3x-meeting04

DOI:

10.4238/vol7-3x-meeting04

Comparison of complete mitochondrial DNA control regions among five Asian freshwater turtle species and their phylogenetic relationships

Authors: Y. Jiang, L.W. Nie, Z.F. Huang, W.X. Jing, L. Wang, L. Liu and X.T. Dai

The complete mitochondrial DNA (mtDNA) control regions (CR), cytochrome b (Cyt b), NADH dehydrogenase subunit 4 (ND4) and cytochrome coxidase subunit I (CO I) genes of four Asian freshwater turtles, Mauremys japonica, Ocadia sinensis, M. mutica, and Annamemys annamensis, were sequenced using universal PCR and long-PCR techniqu.. Read More»

Genet. Mol. Res. 10(3):

cDNA, genomic sequence cloning and overexpression of ribosomal protein gene L9 (rpL9) of the giant panda (Ailuropoda melanoleuca)

Authors: W.R. Hou, Y.L. Hou, G.F. Wu, Y. Song, X.L. Su, B. Sun and J. Li

The ribosomal protein L9 (RPL9), a component of the large subunit of the ribosome, has an unusual structure, comprising two compact globular domains connected by an α-helix; it interacts with 23 S rRNA. To obtain information about rpL9 of Ailuropoda melanoleuca (the giant panda) we designed primers based on the known mam.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1159

DOI:

10.4238/vol10-3gmr1159

Simplified preparation of a DNA ladder using PCR

Authors: T.-Y. Wang, L. Wang and F. Wang

Serving as a DNA molecular weight standard, the DNA ladder has been widely used in molecular biology applications. We developed a simple method for the preparation of a DNA marker, which involves designing primers to amplify 100- to 1000-bp DNA fragments using lambda DNA as a template for polymerase chain reaction, followed by.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1177

DOI:

10.4238/vol10-3gmr1177

Characters that differ between diploid and haploid honey bee (Apis mellifera) drones

Authors: Matthias Herrmann, Tina Trenzcek, Hartmut Fahrenhorst and Wolf Engels

Diploid males have long been considered a curiosity contradictory to the haplo-diploid mode of sex determination in the Hymenoptera. In Apis mellifera, ‘false’ diploid male larvae are eliminated by worker cannibalism immediately after hatching. A ‘cannibalism substance’ produced by diploid drone larvae .. Read More»

Genet. Mol. Res. 4(4):