Dna

A modified method for high-quality DNA extraction for molecular analysis in cereal plants

Authors: Z.H. Pervaiz, N.A. Turi, I. Khaliq, M.A. Rabbani and S.A. Malik

Cereal crops that have rigid non-cellulose components in the cell wall tissues of leaves and high starch and protein content in grains face limitations in DNA extraction. Advanced molecular genetic techniques such as mapping and marker-assisted selection programs require pure and quick DNA extraction. In this study, we developed methods for isolating high.. Read More»

Genet. Mol. Res. 10(3):

vol10-3gmr1346

DOI:

10.4238/vol10-3gmr1346

Allele frequencies and genetic diversity in two groups of wild tufted capuchin monkeys (Cebus apella nigritus) living in an urban forest fragment

Authors: Jeanne Margareth Jimenes Amaral, Aguinaldo Luiz Sim�?µes and David De Jong

There have been numerous studies genetically characterizing Old World Primates using microsatellites. However, few studies have been made of New World species and none on free-ranging Cebus apella, even though it is probably the most widely distributed species of monkey in the New World. The paucity of studies is due, in part,.. Read More»

Genet. Mol. Res. 4(4):

Detecting multiple DNA human profile from a mosquito blood meal

Authors: K.C.N. Rab�?ªlo, C.M.R. Albuquerque, V.B. Tavares, S.M. Santos, C.A. Souza, T.C. Oliveira, R.R. Moura, L.A.C. Brand�?£o and S. Crovella

Criminal traces commonly found at crime scenes may present mixtures from two or more individuals. The scene of the crime is important for the collection of various types of traces in order to find the perpetrator of the crime. Thus, we propose that hematophagous mosquitoes found at crime scenes can be used to perform genetic testing of human blood and aid.. Read More»

Genet. Mol. Res. 15(3):

gmr.15037547

DOI:

10.4238/gmr.15037547

Genetic characterization of European-Zebu composite bovine using RFLP markers

Authors: Erica Perez Marson, Jos�?© Bento Sterman Ferraz, Fl�?¡vio Vieira Meirelles, J�?ºlio C�?©sar de Carvalho Balieiro, Joanir Pereira Eler, Lu�?­s Gustavo Girardi Figueiredo and Gerson Barreto Mour�?£o

A population of 370 European-Zebu composite beef heifers, consisting of six different breed compositions (A-F), were characterized genetically, using RFLP markers of luteinizing hormone receptor (LHR) and follicle-stimulating hormone receptor (FSHR) genes. Our objectives were to genetically characterize this population and to .. Read More»

Genet. Mol. Res. 4(3):

Improved protocol for extracting genomic DNA from frozen formalin-fixed tissue resulting in high-quality whole mtDNA

Authors: H.W. Wang, Y. Xu, H.F. Zhang, Y.J. Zeng, L. Ren, Y.L. Miao, H.Y. Luo and K.H. Wang

Formalin fixation and paraffin embedding is widely used for convenient and long-term storage of tumor tissue and precious sources to perform genetic studies. However, DNA fragmentation is one of the major flaws of genomic DNA isolation from formalin fixation tissues, which limits its further usage. Here, we present an improved method for isolating high-qu.. Read More»

Genet. Mol. Res. 15(3):

gmr.15037972

DOI:

10.4238/gmr.15037972

Development and significance of SCAR marker QG12-5 for Canarium album (Lour.) Raeusch by molecular cloning from improved RAPD amplification

Authors: J.L. Cheng, Z.C. Yin Z.Q. Mei, C.L. Wei, H.C. Chen, X.S. Wu and J.J. Fu

Sequence-characterized amplified region (SCAR) is a valuable molecular marker for the genetic identification of any species.This marker is mainly derived from molecular cloning of random amplified polymorphic DNA (RAPD). We have previously reported the use of an improved RAPD technique for the genetic characterization of different samples of Canarium albu.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038347

DOI:

10.4238/gmr.15038347

Genetic diversity of ITS sequences of Bursaphelenchus xylophilus

Authors: J.M.S. Cardoso L. Fonseca I. Abrantes

The sequence variation of internal transcribed spacer (ITS) regions of ribosomal DNA has been routinely used for species identification and species-level phylogeny of the pinewood nematode, Bursaphelenchus xylophilus. In this study, the intraspecies ITS genetic diversity of B. xylophilus was evaluated. Three pinewood nematode isolates from the United Stat.. Read More»

Genet. Mol. Res. 11(4):

2012.October.15.1

DOI:

10.4238/2012.October.15.1

Mitochondrial DNA mutations may not be frequent in patients with aplastic anemia - Genet. Mol. Res. 11 (3): 2130-2137 â�?�?Complete sequence analysis of mitochondrial DNA of aplastic anemia patientsâ�?

Authors: J. Zhang N. Li J.-Y. Lin Q. Li J. Hai D.-W. Xu X.-L. Qian

Genet. Mol. Res. 11(4):

2012.December.21.2

DOI:

10.4238/2012.December.21.2

A protocol for high-quality genomic DNA extraction from legumes

Authors: I.O. Agbagwa S. Datta P.G. Patil P. Singh N. Nadarajan

Current DNA extraction protocols, which require liquid nitrogen, lyophilization and considerable infrastructure in terms of instrumentation, often impede the application of biotechnological tools in less researched crops in laboratories in developing countries. We modified and optimized the existing CTAB method for plant genomic DNA extraction by avoiding li.. Read More»

Genet. Mol. Res. 11(4):

2012.September.14.1

DOI:

10.4238/2012.September.14.1

Trans-translation ensures timely initiation of DNA replication and DnaA synthesis in Escherichia coli

Authors: W. Wurihan, W. Wunier, H. Li, L.F. Fan and M. Morigen

The trans-translation pathway, mediated by the transfer messenger RNA (tmRNA; encoded by the ssrA gene) and the SmpB protein (tmRNA-binding protein expressed in Salmonella enterica), which is conserved in bacteria, is required for various cellular processes. A previous study has shown that trans-translation is required to ensu.. Read More»

Genet. Mol. Res. 15(3):

gmr.15038407

DOI:

10.4238/gmr.15038407