Dna
Novel transcripts of human cytomegalovirus clinical strain found by cDNA library screening
Authors: Y.P. Ma, Q. Ruan, Y.H. Ji, N. Wang, M.L. Li, Y. Qi, R. He, Z.R. Sun and G.W. Ren
Human cytomegalovirus (HCMV) is a double-stranded DNA virus with the largest genome (~235 kb) of the known human herpes viruses. The coding potential and transcript structures of most HCMV predicted genes have not been identified. New or unknown genes could exist in clinical strains. The SMART (switching mechanism at 5êÃ.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1059
- DOI:
- 10.4238/vol10-2gmr1059
Estimation of taurindicine hybridization of American Zebu cattle in Brazil
Authors: P. Ripamonte, M. Baccaglini, A.S.M. Cesar, L.G.G. Figueiredo, J.C.C. Balieiro, A.R. Caetano and F.V. Meirelles
Our objective was to estimate Bos primigenius taurus introgression in American Zebu cattle. One hundred and four American Zebu (Nellore) cattle were submitted to mtDNA, microsatellite and satellite analysis. Twenty-three alleles were detected in microsatellite analysis, averaging 4.6 ± 1.82/locus. Variance component com.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.17.1
- DOI:
- 10.4238/2012.February.17.1
Position effect variegation and epigenetic modification of a transgene in a pig model
Authors: Z. Yin, Q.R. Kong, Z.P. Zhao, M.L. Wu, Y.S. Mu, K. Hu1 and Z.H. Liu
Sequences proximal to transgene integration sites are able to regulate transgene expression, resulting in complex position effect variegation. Position effect variegation can cause differences in epigenetic modifications, such as DNA methylation and histone acetylation. However, it is not known which factor, position effect or.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.16.1
- DOI:
- 10.4238/2012.February.16.1
Simple and rapid protocol for the isolation of PCR-amplifiable DNA from medicinal plants
Authors: A.A. Alatar, M.A. Mahmoud, S.A. Al-Sohaibani and K.A. Abd-Elsalam
Medicinal plant species has a valuable economic importance because of its usage as pharmaceuticals, nutritional, as well as its use in popular medication. For DNA-based techniques, nanogram quantities of the purified DNA are requisite to amplify and yield sufficient amounts of PCR products. SDS-based DNA isolation method was u.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.13.1
- DOI:
- 10.4238/2012.February.13.1
Genetic structure based on nuclear and chloroplast microsatellite loci of Solanum lycocarpum A. St. Hil. (Solanaceae) in Central Brazil
Authors: K. Martins, L.J. Chaves, R. Vencovsky and P.Y. Kageyama
Solanum lycocarpum (Solanaceae) is a woody species found in the Brazilian Cerrado. The flowers are pollinated by Xylocopa spp bees, and seeds are dispersed by mammals with distinct home range sizes. As a consequence, relative contributions of pollen and seeds to overall gene flow can vary according to different spatial scales... Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1046
- DOI:
- 10.4238/vol10-2gmr1046
Optimization of a multiplex minisequencing protocol for population studies and medical genetics
Authors: Cl�¡udia M.B. Carvalho and S�©rgio D.J. Pena
Several technologically sophisticated high-throughput techniques have been recently developed for the study of human single nucleotide polymorphisms and the diagnosis of point mutations in human diseases. However, there is also a need for simple and inexpensive techniques suitable for clinical services and small research labor.. Read More»
- Genet. Mol. Res. 4(2):
DNA barcoding and phylogenetic relationships in Timaliidae
Authors: Z.H. Huang and D.H. Ke
The Timaliidae, a diverse family of oscine passerine birds, has long been a subject of debate regarding its phylogeny. The mitochondrial cytochrome c oxidase subunit I (COI) gene has been used as a powerful marker for identification and phylogenetic studies of animal species. In the present study, we analyzed the COI barcodes of 71 species from 21 genera .. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.1.11
- DOI:
- 10.4238/2015.June.1.11
Development of a species-specific sequence-characterized amplified region marker for roses
Authors: S. Riaz, B. Sadia, F.S. Awan, I.A. Khan, H.A. Sadaqat and I.A. Khan
DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.February.24.3
- DOI:
- 10.4238/2012.February.24.3
Evaluation of the biodegradability of petroleum in microcosm systems by using mangrove sediments from Camamu Bay, Bahia, Brazil
Authors: A.C.F. Santos, R.P. Rezende, M. Brendel, S.S. Souza, A.C.S. Gon�§alves and J.C.T. Dias
We investigated the biodegradability of oil in mangrove sediment from Camamu Bay and measured its effect on the bacterial community. Microcosms of mangrove sediment were contaminated with 0.1, 0.5, 1, 2, and 5% (w/v) oil, and the microbial activity was compared to that in uncontaminated sediment. The evolution of CO2 and gas c.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.24.9
- DOI:
- 10.4238/2014.March.24.9
A modified acidic approach for DNA extraction from plant species containing high levels of secondary metabolites
Authors: M.M. Cavallari, M.V.B.M. Siqueira, T.M. Val, J.C. Pavanelli, M. Monteiro, C. Grando, J.B. Pinheiro, M.I. Zucchi and M.A. Gimenes
Purified genomic DNA can be difficult to obtain from some plant species because of the presence of impurities such as polysaccharides, which are often co-extracted with DNA. In this study, we developed a fast, simple, and low-cost protocol for extracting DNA from plants containing high levels of secondary metabolites. This pro.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.25.13
- DOI:
- 10.4238/2014.August.25.13