Dna
Genetic distances and phylogenetic trees of different Awassi sheep populations based on DNA sequencing
Authors: R.M. Al-Atiyat and R.S. Aljumaah
This study aimed to estimate evolutionary distances and to reconstruct phylogeny trees between different Awassi sheep populations. Thirty-two sheep individuals from three different geographical areas of Jordan and the Kingdom of Saudi Arabia (KSA) were randomly sampled. DNA was extracted from the tissue samples and sequenced u.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.26.6
- DOI:
- 10.4238/2014.August.26.6
Molecular cloning, sequence analysis, prokaryotic expression, and function prediction of foot-specific peroxidase in Hydra magnipapillata Chinese strain
Authors: H.C. Pan, H.Q. Yang, F.X. Zhao and X.C. Qian
The cDNA sequence of foot-specific peroxidase PPOD1 from the Chinese strain of Hydra magnipapillata was cloned by reverse transcription-polymerase chain reaction. The cDNA sequence contained a coding region with an 873-bp open reading frame, a 31-bp 5'-untranslated region, and a 36-bp 3'-untranslated region. The struc.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.August.28.6
- DOI:
- 10.4238/2014.August.28.6
Chromosomal localization and partial sequencing of the 18S and 28S ribosomal genes from Bradysia hygida (Diptera: Sciaridae)
Authors: V.P. Gaspar, E.L.T. Shimauti and M.A. Fernandez
In insects, ribosomal genes are usually detected in sex chromosomes, but have also or only been detected in autosomal chromosomes in some cases. Previous results from our research group indicated that in Bradysia hygida, nucleolus organizer regions were associated with heterochromatic regions of the autosomal C chromosome, usi.. Read More»
- Genet. Mol. Res. 13(1):
- 2014.March.26.6
- DOI:
- 10.4238/2014.March.26.6
Molecular cloning, overexpression, purification, and sequence analysis of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide
Authors: L. Fu, Y.L. Hou, X. Ding, Y.J. Du, H.Q. Zhu, N. Zhang and W.R. Hou
The complementary DNA (cDNA) of the giant panda (Ailuropoda melanoleuca) ferritin light polypeptide (FTL) gene was successfully cloned using reverse transcription-polymerase chain reaction technology. We constructed a recombinant expression vector containing FTL cDNA and overexpressed it in Escherichia coli using pET28a plasmi.. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038593
- DOI:
- 10.4238/gmr.15038593
Building the SeqChromMM Markov property atlas of the human genome by analyzing the 200-bp units of the 15 different chromatin regions of ENCODE
Authors: K.-E. Lee and H.-S. Park
We analyzed the publicly available ChromHMM BED files of the ENCODE project and tested the Markov properties of the different chromatin states in the human genome. Nucleotide frequency profiles of regional chromatin segmentations were analyzed, and Markov chains were built to detect Markov properties in the chromatin states of different ChromHMM regions. .. Read More»
- Genet. Mol. Res. 15(3):
- gmr.15038992
- DOI:
- 10.4238/gmr.15038992
Highly efficient one-step PCR-based mutagenesis technique for large plasmids using high-fidelity DNA polymerase
Authors: H. Liu, R. Ye and Y.Y. Wang
Gene mutation plays an important role in molecular biological studies. A highly efficient one-step polymerase chain reaction-based mutagenesis technique for site-directed mutagenesis was developed in this study. One complementary pair of primers was designed that contained the desired mutations in the middle of the primers. The amplification products of m.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.15.10
- DOI:
- http://dx.doi.org/10.4238/2015.April.15.10
Adaption of SYBR Green-based reagent kit for real-time PCR quantitation of GC-rich DNA
Authors: G.J. Chang, H.M. Seyfert and X.Z. Shen
In the mammalian genome, approximately 50% of all genes are controlled by promoters with high GC contents. Analyzing the epigenetic mechanisms regulating their expression is difficult. Hence, we examined a method for stable quantification of such GC-rich DNA sequences. Quantification of DNA during real-time PCR is often based on reagent kits containing th.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.20
- DOI:
- 10.4238/2015.July.28.20
Promoter and first exon methylation regulate porcine FASN gene expression
Authors: K.R. Long, J.D. Ma, L. Chen, P.J. Wang, D.F. He, M.Z. Li and X.W. Li
DNA methylation is a stable epigenetic mark mediating gene expression. Methylation is crucial for diverse biological processes, including aging and embryo development. FASN (fatty acid synthase) plays an important role in de novo lipogenesis, through catalyzing the reductive synthesis of long-chain fatty acids. In this study, we investigated the FASN gene.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.July.28.11
- DOI:
- 10.4238/2015.July.28.11
Comparison of five DNA extraction methods for molecular analysis of Jerusalem artichoke (Helianthus tuberosus)
Authors: T. Mornkham, P.P. Wangsomnuk, P. Wangsomnuk, S. Jogloy, A. Pattanothai and Y.B. Fu
DNA extraction is an essential step for molecular analysis of an organism, but it is difficult to acquire a sufficient amount of pure DNA from plant tissue with high levels of phenolic compounds, carbohydrates, proteins, and secondary metabolites. Jerusalem artichoke (Helianthus tuberosus) has high levels of such substances. W.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.8.5
- DOI:
- 10.4238/2012.March.8.5
DNA barcoding of Oryx leucoryx using the mitochondrial cytochrome C oxidase gene
Authors: K. Elmeer, A. Almalki, K.A. Mohran, K.N. AL-Qahtani and M. Almarri
The massive destruction and deterioration of the habitat of Oryx leucoryx and illegal hunting have decimated Oryx populations significantly, and now these animals are almost extinct in the wild. Molecular analyses can significantly contribute to captive breeding and reintroduction strategies for the conservation of this endang.. Read More»
- Genet. Mol. Res. 11(1):
- 2012.March.8.2
- DOI:
- 10.4238/2012.March.8.2