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Dna

Plant Genetics   Research Article

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae)

Authors: G. Yu, Y.X. Ma, J.-A. Duan, B.S. Song and Z.Q. He

Angelica sinensis is a highly valued medicinal herb, known as female ginseng that is widely cultivated in China. Although A. sinensis is in great demand due to its multiple medicinal and food applications, its early bolting rate (almost 40%) seriously affects crop quality. To better understand its flowering mechanism, cDNA-amp.. Read More»

Genet. Mol. Res. 11(1):
2012.March.6.2
DOI:
10.4238/2012.March.6.2
Cytogenomics   Research Article

Novel SNPs in the growth arrest and DNA damage-inducible protein 45 alpha gene (GADD45A) associated with meat quality traits in Berkshire pigs

Authors: E.S. Cho, K.T. Lee, J.W. Choi, H.J. Jeon, S.W. Lee, Y.M. Cho and T.H. Kim

This study was conducted to evaluate the porcine gene GADD45A (growth arrest and DNA-damage-inducible protein 45 alpha) as a positional candidate controlling quantitative trait loci (QTL) for meat quality traits on chromosome 6 (SSC6). Four exons of the porcine GADD45A gene were defined from cDNA and BAC clone sequences. A total of 4 single nucleotide pol.. Read More»

Genet. Mol. Res. 14(3):
2015.July.31.6
DOI:
10.4238/2015.July.31.6
Animal Genetics   Research Article

DNA extraction from skins of wild (Hydrochoerus hydrochaeris and Pecari tajacu) and domestic (Sus scrofa domestica) species using a novel protocol

Authors: G.N. Ojeda, P.S. Amavet, E.C. Rueda and P.A. Siroski

Sometimes, commercial products obtained from wild animals are sold as if they were from domestic animals and vice versa. At this point of the productive chain, legal control of possible wildlife products is difficult. Common in the commerce of northern Argentina, skins of two wild species, the carpincho and the collared peccar.. Read More»

Genet. Mol. Res. 11(1):
2012.March.19.1
DOI:
10.4238/2012.March.19.1
Plant Genetics   Research Article

rDNA ITS sequences among morphotypes of Keratell cochlearis, Keratell quadrata and Brachionus forficula (Rotifera)

Authors: Y.L. Ge, Y.L. Xi, J. Ma and D.D. Xu

Morphological variation in rotifers is affected by environmental conditions, making it hard to identify some rotifer taxa. We examined the rDNA ITS sequences of 10 unspined (KCU1-KCU10) and 17 spined (KCS1-KCS17) Keratell cochlearis clones, 26 two-spined (KQT1-KQT26), 18 single-spined (KQS1-KQS18) and 9 unspined (KQU1-KQU9) K... Read More»

Genet. Mol. Res. 11(1):
2012.March.22.7
DOI:
10.4238/2012.March.22.7
Plant Genetics   Research Article

Isolation of high quality and polysaccharide-free DNA from leaves of Dimorphandra mollis (Leguminosae), a tree from the Brazilian Cerrado

Authors: H.A.V. Souza, L.A.C. Muller, R.L. Brand�£o and M.B. Lovato

Dimorphandra mollis (Leguminosae), known as faveiro and fava d’anta, is a tree that is widely distributed throughout the Brazilian Cerrado (a savanna-like biome). This species is economically valuable and has been extensively exploited because its fruits contain the flavonoid rutin, which is used to produce medications f.. Read More»

Genet. Mol. Res. 11(1):
2012.March.22.6
DOI:
10.4238/2012.March.22.6
Plant Genetics   Research Article

Molecular characterization and RAPD analysis of Juniperus species from Iran

Authors: J. Kasaian, J. Behravan, M. Hassany, S.A. Emami, F. Shahriari and M.H. Khayyat

The genus Juniperus L. (Cupressaceae), an aromatic evergreen plant, consists of up to 68 species around the world. We classified five species of Juniperus found in Iran using molecular markers to provide a means for molecular identification of Iranian species. Plants were collected (three samples of each species) from two diff.. Read More»

Genet. Mol. Res. 10(2):
vol10-2gmr1021
DOI:
10.4238/vol10-2gmr1021
Plant Genetics   Research Article

Karyotype asymmetry in Cynodon Rich. (Poaceae) accessions

Authors: R.B. Chiavegatto, C.M.P. Paula, F. Souza Sobrinho, F.R.G. Benites and V.H. Techio

Cynodon is a genus of plants with forage potential that has attracted the interest of breeders. These species have high morphological variability in a large number of varieties and cytotypes, hampering identification. This study aimed to determine the karyotype asymmetry index among accessions of Cynodon to discriminate betwee.. Read More»

Genet. Mol. Res. 15(4):
gmr15049152
DOI:
10.4238/gmr15049152
Human Genetics   Research Article

Using Markov chains of nucleotide sequences as a possible precursor to predict functional roles of human genome: a case study on inactive chromatin regions

Authors: K.-E. Lee, E.-J. Lee, and H.-S. Park

Recent advances in computational epigenetics have provided new opportunities to evaluate n-gram probabilistic language models. In this paper, we describe a systematic genome-wide approach for predicting functional roles in inactive chromatin regions by using a sequence-based Markovian chromatin map of the human genome. We demonstrate that Markov chains of.. Read More»

Genet. Mol. Res. 15(3):
gmr.15039004
DOI:
10.4238/gmr.15039004
Medical Genetics   Research Article

Cytotoxicity and DNA damage in mouse macrophages exposed to silica nanoparticles

Authors: H. Yang1, Q.Y. Wu1,2, C.S. Lao1, M.Y. Li1, Y. Gao1, Y. Zheng1 and B. Shi3

Silica (SiO2) nanoparticles are being progressively applied in various applications, including cosmetics, food technology, and medical diagnostics. Although crystalline SiO2 is a known carcinogen, the carcinogenicity of SiO2 nanoparticles remains unclear. Here, we assessed the cytotoxic effects and DNA injury induced by exposu.. Read More»

Genet. Mol. Res. 15(3):
gmr.15039005
DOI:
10.4238/gmr.15039005
Microbial Genetics   Research Article

Search for methylation-sensitive amplification polymorphisms in mutant figs

Authors: M.G.F. Rodrigues, A.B.G. Martins, B.W. Bertoni, A. Figueira and S. Giuliatti

Fig (Ficus carica) breeding programs that use conventional approaches to develop new cultivars are rare, owing to limited genetic variability and the difficulty in obtaining plants via gamete fusion. Cytosine methylation in plants leads to gene repression, thereby affecting transcription without changing the DNA sequence. Previous studies using random amp.. Read More»

Genet. Mol. Res. 12(3):
2013.July.8.8
DOI:
10.4238/2013.July.8.8