Dna
Comparison of methods for high quantity and quality genomic DNA extraction from raw cow milk
Authors: T. Usman, Y. Yu, C. Liu, Z. Fan and Y. Wang
Isolation of sufficient quantities of high quality DNA is a prerequisite for molecular studies. Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk a difficult medium for extracting large amounts of quality DNA. We optimized, evaluated and compared three methods, Modified Nucleospin Blood Ki.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.29.10
- DOI:
- 10.4238/2014.April.29.10
The first assess of the haplotypes from COI gene sequences in species of spittlebugs (Cicadomorpha: Hemiptera) and aquatic true bugs (Gerromorpha and Nepomorpha: Hemiptera) in Brazil
Authors: M.M.U. Castanhole1, S.R.C. Marchesin2, L.L.V. Pereira1, F.F.F. Moreira3, J.F. Barbosa3, J.R. Val�©rio4 and M.M. Itoyama1
We made the first analysis of the COI gene sequences of 22 species of spittlebugs and aquatic true bugs sampled in São Paulo State (Brazil) and used this information to determine the variability within these groups. Considering each codon position, we observed that the third base was the most variable, and the first base was the most conserved. Amo.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.7.12
- DOI:
- 10.4238/2013.November.7.12
Transcriptional regulation of CD4 gene expression in porcine kidney epithelial cells by virus-like double-stranded RNA and DNA methyltransferase inhibitor
Authors: X.S. Wang, H. Ao, L.W. Zhai, L.J. Bai, W.Y. He, C.D. Wang, Y. Yu
The effects of virus-like double-stranded RNA (dsRNA, PolyI:C) and DNA methyltransferase inhibitor (Aza-CdR) on CD4 gene expression were investigated in a porcine kidney cell line (PK15). We found that expression levels of TLR3 and IFNα were significantly upregulated by PolyI:C, compared to the untreated PK15 cells, whic.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.13
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.13
Genetic diversity of local Yunnan chicken breeds and their relationships with Red Junglefowl
Authors: J.L. Huo, G.S. Wu, T. Chen, H.L. Huo, F. Yuan, L.X. Liu, C.R. Ge and Y.W. Miao
Yunnan is situated in the Southwest China and encompasses regions having high biodiversity, including habitats for several ancestral species of domestic animals such as chicken. Domestic chickens in Yunnan were kept by peoples of varied ethnic and economic backgrounds living in highly varied geographic environments. To identif.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.April.29.16
- DOI:
- http://dx.doi.org/10.4238/2014.April.29.16
Rapid method for DNA extraction from the honey bee Apis mellifera and the parasitic bee mite Varroa destructor using lysis buffer and proteinase K
Authors: M.R.C. Issa1, V.L.C. Figueiredo2, D. De Jong1, C.H. Sakamoto1 and Z.L.P. Sim�µes2
We developed a rapid method for extraction of DNA from honey bees, Apis mellifera, and from the parasitic bee mite, Varroa destructor. The advantages include fast processing and low toxicity of the substances that are utilized. We used lysis buffer with nonionic detergents to lyse cell walls and proteinase K to digest proteins. We tested whole thorax, tho.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.22.4
- DOI:
- 10.4238/2013.October.22.4
A tiered barcode authentication tool to differentiate medicinal Cassia species in India
Authors: N. Purushothaman, S.G. Newmaster, S. Ragupathy, N. Stalin, D. Suresh, D.R. Arunraj, G. Gnanasekaran, S.L. Vassou, D. Narasimhan and M. Parani
DNA barcoding is a desirable tool for medicinal product authentication. DNA barcoding is a method for species identification using short DNA sequences that are conserved within species, but variable between species. Unlike animals, there is no single universal DNA barcode locus for plants. Coding markers, matK and rbcL, and no.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.16.4
- DOI:
- 10.4238/2014.April.16.4
Homocysteine induces blood vessel global hypomethylation mediated by LOX-1
Authors: X.L. Yang, J. Tian, Y. Liang, C.J. Ma, A.N. Yang, J. Wang, S.C. Ma, Y. Cheng, X. Hua and Y.D. Jiang
Homocysteine (Hcy) is an independent risk factor of atherosclerosis through its involvement with the methionine cycle. In this study, we aimed to determine the blood vessel global methylation rate in Hcy-induced atherosclerosis in apolipoprotein-E-deficient (ApoE-/-) mice, and to explore the possible mechanism of this change i.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.May.16.2
- DOI:
- http://dx.doi.org/10.4238/2014.May.16.2
Full-length cDNA cloning and structural characterization of preproinsulin in Alligator sinensis
Authors: R. Zhang, S.Z. Zhang, E. Li, C. Wang, C.L. Wang and X.B. Wu
Insulin is an important endocrine hormone that plays a critical physiological role in regulating metabolism and glucostasis in vertebrates. In this study, the complete cDNA of Alligator sinensis preproinsulin gene was cloned for the first time by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods; the amino acid s.. Read More»
- Genet. Mol. Res. 13(4):
Identification of orchardgrass (Dactylis glomerata L.) cultivars by using simple sequence repeat markers
Authors: L.-F. Jiang1, X.-Q. Zhang1, X. Ma1, L.-K. Huang1, W.-G. Xie2, Y.-M. Ma1 and Y.-F. Zhao1
The accurate identification of orchardgrass (Dactylis glomerata L.) cultivars is necessary to ensure purity for consumers, the effective utilization of cultivars, and to protect the intellectual property for breeders. Therefore, this study aimed to use SSR to construct DNA fingerprinting of orchardgrass cultivars. The genetic diversity of 32 orchardgrass .. Read More»
- Genet. Mol. Res. 12(4):
Overexpression, purification, and pharmacologic evaluation of anticancer activity of ribosomal protein L24 from the giant panda (Ailuropoda melanoleuca)
Authors: Y.L. Hou, X. Ding, W. Hou, B. Song, T. Wang, F. Wang, J. Li, J. Zhong, T. Xu, B.X. Ma, H.Q. Zhu, J.H. Li and J.C. Zhong
The ribosomal protein L24 (RPL24) belongs to the L24E family of ribosomal proteins and is located in the cytoplasm. The purpose of this study was to investigate the structure and anti-cancer function of RPL24 of the giant panda (Ailuropoda melanoleuca). The complementary DNA of RPL24 was cloned successfully using reverse transcription-polymerase chain rea.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.18.11
- DOI:
- 10.4238/2013.October.18.11