Dna
Development of microsatellite markers for Mytilus coruscus (Mytilidae), an economically important mussel in the East China Sea
Authors: B.-Y. Guo, C.-W. Wu, C.-L. Liu, A.-Y. Zhu and Y.-Y. Ye
Twelve new polymorphic microsatellite loci were developed for the hard-shelled mussel, Mytilus coruscus. In 32 individuals from a wild population of coastal Zhoushan, Zhejiang Province, China, the number of alleles at these loci varied from 3 to 15, with a mean of 5.667. The mean observed and expected heterozygosities were 0.6927 and 0.6591, respectively... Read More»
- Genet. Mol. Res. 12(3):
- 2013.July.30.5
- DOI:
- 10.4238/2013.July.30.5
Identification of apple cultivars on the basis of simple sequence repeat markers
Authors: G.S. Liu, Y.G. Zhang, R. Tao, J.G. Fang and H.Y. Dai
DNA markers are useful tools that play an important role in plant cultivar identification. They are usually based on polymerase chain reaction (PCR) and include simple sequence repeats (SSRs), inter-simple sequence repeats, and random amplified polymorphic DNA. However, DNA markers were not used effectively in the complete ide.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.3
- DOI:
- 10.4238/2014.September.12.3
DNA barcoding and evolutionary relationships of the Phasianidae family in China
Authors: Z.H. Huang and D.H. Ke
A DNA barcode is a short sequence of standardized genomic region that is specific to a species. According to studies of bird species, the 694-bp sequence of the mitochondrial gene encoding cytochrome c oxidase 1 (COI) is extremely useful for species identification and phylogeny. In the present study, we analyzed the COI barcod.. Read More»
- Genet. Mol. Res. 13(3):
- 2014.September.12.7
- DOI:
- 10.4238/2014.September.12.7
Development of polymorphic microsatellite loci for a new fish species, Chinese sillago (Sillago sinica)
Authors: N. Song, L.Y. Wang, L.N. Yin, Z.Q. Han and T.X. Gao
Sillago sinica is a newly identified species belonging to Sillaginidae, Perciforms, and was found along the coast of China in 2011. In the present study, 81 microsatellite loci were isolated from an enriched genomic library, and 24 positive clones containing microsatellite repeats had adequate flanking sequences for the develo.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027212
- DOI:
- 10.4238/gmr.15027212
Structure of mitochondrial DNA control region and genetic diversity of Moschus berezovskii populations in Shaanxi Province
Authors: H. Feng, C.L. Feng, Y. Huang and J. Tang
In China, Moschus berezovskii (forest musk deer), a first-class national protected animal, was earlier widely distributed. However, wild populations of the forest musk deer have declined because of human activity and habitat loss. In order to gather useful information for its conservation and management, we investigated the ge.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027578
- DOI:
- 10.4238/gmr.15027578
Prediction efficiency of PITX2 DNA methylation for prostate cancer survival
Authors: Z.M. Luan, H. Zhang and X.L. Qu
This study determined the level of PITX2 methylation in prostate cancer and benign tissues and its relationship with the postoperative survival rate. Forty-four patients with prostate cancer who underwent radical prostatectomy and 43 patients with benign prostatic hyperplasia were selected. DNA was extracted from the tissues a.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15026750
- DOI:
- 10.4238/gmr.15026750
Efficiency of RAPD versus SSR markers for determining genetic diversity among popcorn lines
Authors: A.A. Leal, C.A. Mangolin, A.T. do Amaral J�ºnior, L.S.A. Gon�§alves, C.A. Scapim, A.S. Mott, I.B.O. Eloi, V. Cordov�©s and M.F.P. da Silva
Using only one type of marker to quantify genetic diversity generates results that have been questioned in terms of reliability, when compared to the combined use of different markers. To compare the efficiency of the use of single versus multiple markers, we quantified genetic diversity among 10 S7 inbred popcorn lines using .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr692
- DOI:
- 10.4238/vol9-1gmr692
Evaluation of the taxonomic status of water dropwort (Oenanthe, Apiaceae) accessions from East Asia based on nuclear rDNA internal transcribed spacer sequences
Authors: S. Fu, L.N. Li, Z.C. Long, W.D. Ke, A.H. Ye, Y.H. Guo and J.M. Chen
Oenanthe L. is a taxonomically complex genus, several species of which have long been used as vegetables and traditional medicines in East Asia. In order to clarify the taxonomic status of Oenanthe accessions and provide baseline data for the sustainable use of its genetic resources, we examined sequence variations in the inte.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027363
- DOI:
- 10.4238/gmr.15027363
DNA repair mutant pso2 of Saccharomyces cerevisiae is sensitive to intracellular acetaldehyde accumulated by disulfiram-mediated inhibition of acetaldehyde dehydrogenase
Authors: M. Brendel, G. Marisco, I. Ganda, R. Wolter and C. Pungartnik
Blocking aldehyde dehydrogenase with the drug disulfiram leads to an accumulation of intracellular acetaldehyde, which negatively affects the viability of the yeast Saccharomyces cerevisiae. Mutants of the yeast gene PSO2, which encodes a protein specific for repair of DNA interstrand cross-links, showed higher sensitivity to .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr695
- DOI:
- 10.4238/vol9-1gmr695
Evaluation of candidate barcoding markers in Orinus (Poaceae)
Authors: X. Su, Y.P. Liu, Z. Chen and K.L. Chen
Orinus is an alpine endemic genus of Poaceae. Because of the imperfect specimens, high level of intraspecific morphological variability, and homoplasies of morphological characters, it is relatively difficult to delimitate species of Orinus by using morphology alone. To this end, the DNA barcoding has shown great potential in .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027714
- DOI:
- 10.4238/gmr.15027714