Dna
A rapid method for isolation of total DNA from pathogenic filamentous plant fungi
Authors: D. GonzÃ?¡lez-Mendoza, R. Argumedo-Delira, A. Morales-Trejo, A. Pulido-Herrera, L. Cervantes-DÃ?Âaz, O. Grimaldo-Juarez and A. AlarcÃ?³n
DNA isolation from some fungal organisms of agronomic importance is difficult because they have cell walls or capsules that are relatively unsusceptible to lysis. We have developed a fast DNA isolation protocol for Fusarium oxysporum, which causes fusarium wilt disease in more than 100 plant species, and for Pyrenochaeta terre.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr680
- DOI:
- 10.4238/vol9-1gmr680
New efficient DNA extraction method to access the microbiome of Ricinus communis seeds
Authors: C.D. Santos Jr., A.C.C. Dias, I.M.R. Amaral, A.M. Bonetti and T.A. Campos
Ricinus communis (castor bean) seeds are used to produce an alcohol-soluble oil that is used in more than 400 industrial processes. Despite its economic importance, there has been little research on the endophytic microbiota of castor bean seeds. This microbiota is important for plant metabolic processes and may have considerable biotechnological potentia.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.February.28.23
- DOI:
- 10.4238/2013.February.28.23
Enzyme activity and morphological change in the spleens of crucian carp in the Yongcheng coal mine subsidence area, China
Authors: Y.F. Yan, J.Y. Yang, and J.Y. Lin
The goal of the current study was to investigate the effects of pollution on aquatic organisms in the Yongcheng coal mine subsidence area. Crucian carp (Carassius auratus) were collected from Yongcheng natural fishpond (experimental group) and Tianmu Lake (control group), and the spleens were isolated for analysis. Subsequentl.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027782
- DOI:
- 10.4238/gmr.15027782
Investigation of NQO1 genetic polymorphism, NQO1 gene expression and PAH-DNA adducts in ESCC. A case-control study from Iran
Authors: H.A. Marjani, F. Biramijamal, N. Rakhshani, A. Hossein-Nezhad and R. Malekzadeh
We evaluated the effect of NQO1 genetic variation on PAH-DNA adducts in esophageal squamous cell carcinoma (ESCC) in northeast Iran. Golestan Province in northeast of Iran has one of the highest esophageal cancer incidences in the world. The study included 93 ESCC cases and 50 control individuals who were seen at the clinical .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr693
- DOI:
- 10.4238/vol9-1gmr693
Optimization of DNA extraction from seeds and fresh leaf tissues of wild marigold (Tagetes minuta) for polymerase chain reaction analysis
Authors: I. Shahzadi, R. Ahmed, A. Hassan and M.M. Shah
Tagetes, a genus of flowering marigolds in the family Asteraceae (Compositeae), is reported to be a medicinal plant with hypotensive, spasmolytic, anti-inflammatory, antimicrobial, and antifungal properties. Tagetes minuta characteristically contains high concentrations of essential oils, flavonoids, polyphenols, and polysacch.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr747
- DOI:
- 10.4238/vol9-1gmr747
DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA
Authors: S. Tabasum, F.A. Khan, S. Nawaz, M.Z. Iqbal and A. Saeed
DNA profiles of 40 sugarcane genotypes were constructed with 30 RAPD markers. Sugarcane genotypes of both Saccharum officinarum and S. barberi were included in this study. Multiple alleles were detected from each RAPD; there was a high level of polymorphism. On average, 7.93 alleles were produced per primer, giving a total of .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr709
- DOI:
- 10.4238/vol9-1gmr709
Expression of Epstein-Barr virus antibodies EA-IgG, Rta-IgG, and VCA-IgA in nasopharyngeal carcinoma and their use in a combined diagnostic assay
Authors: Y. Li, K. Wang, S.K.Yin, H.L. Zheng and D.L. Min
Epstein-Barr virus (EBV) infection is closely associated with nasopharyngeal carcinoma, which can be monitored by the levels of Rta protein antibody IgG (Rta-IgG), early antigen antibody (EA-IgG), and viral capsid antibody (VCA-IgA). In the present study, we investigated the serum levels of Rta-IgG, EA-IgG, and VCA-IgA in naso.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017368
- DOI:
- 10.4238/gmr.15017368
Comparative study of the cytogenetic and molecular characteristics of Triatoma maculata and T. pseudomaculata (Heteroptera, Triatominae)
Authors: P.P. Mendon�§a
Triatomines are hematophagous insects of great concern in public health because they are vectors of Trypanosoma cruzi, a protozoan that causes Chagas disease. Triatomines are also of great genetic interest, because they possess holocentric chromosomes and undergo an unusual form of meiosis with post-reductional segregation of .. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1TA029
- DOI:
- 10.4238/vol9-1TA029
Efficient human paternity testing with a panel of 40 short insertion-deletion polymorphisms
Authors: J.R. Pimenta and S.D.J. Pena
We developed a panel of 40 multiplexed short insertion-deletion (indel) polymorphic loci with widespread chromosomal locations and allele frequencies close to 0.50 in the European population. We genotyped these markers in 360 unrelated self-classified White Brazilians and 50 mother-child-probable father trios with proven pater.. Read More»
- Genet. Mol. Res. 9(1):
- vol9-1gmr838
- DOI:
- 10.4238/vol9-1gmr838
Genetic diversity of Palestine landraces of faba bean (Vicia faba) based on RAPD markers
Authors: R. Basheer-Salimia, M. Shtaya, M. Awad, J. Abdallah and Y. Hamdan
Until now, neither phenotypic nor molecular approaches have been used to characterize the landraces of Palestine faba beans (Vicia faba). We used PCR-based RAPD markers to determine the genetic diversity and relatedness among 26 Palestinian faba bean landraces (traditional farmers’ varieties) from 8 localities in the West Bank, Palestine. In tests w.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.3.8
- DOI:
- 10.4238/2013.September.3.8