Dna
DNA detection of Clostridium difficile infection based on real-time resistance measurement
Authors: C. Liu, D.N. Jiang, G.M. Xiang, F.K. Luo, L.L. Liu, J.C. Yu and X.Y. Pu
We used a newly developed electrochemical method, real-time resistance measurement, based on loop-mediated isothermal amplification (LAMP), with real-time resistance monitoring and derivative analysis. DNA extracted from specimens was amplified through LAMP reaction. The 2 products of LAMP, DNA and pyrophosphate, both are negative ions; they combine with .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.3.6
- DOI:
- 10.4238/2013.September.3.6
Molecular characterization of Aspergillus flavus and aflatoxin contamination of wheat grains from Saudi Arabia
Authors: A.S. Al-Wadai, M.R. Al-Othman, M.A. Mahmoud and A.R.M. Abd El-Aziz
Twelve species belonging to six fungal genera were found to be associated with wheat (Triticum aestivum L.) grain samples collected from three main regions in Saudi Arabia. The most common genera (average frequency) were Aspergillus (14.3%), Fusarium (29.1%), Penicillium (9.3%), and Alternaria (8.2%). Nineteen isolates of Aspergillus flavus were screened .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.3.10
- DOI:
- 10.4238/2013.September.3.10
Sperm quality and DNA damage in men from Jilin Province, China, who are occupationally exposed to ionizing radiation
Authors: D.D. Zhou, J.L. Hao, K.M. Guo, C.W. Lu and X.D. Liu
Long-term radiation exposure affects human health. Ionizing radiation has long been known to raise the risk of cancer. In addition to high doses of radiation, low-dose ionizing radiation might increase the risk of cardiovascular disease, lens opacity, and some other non-cancerous diseases. Low- and high-dose exposures to ioniz.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15018078
- DOI:
- 10.4238/gmr.15018078
Population genetic structure and historical demography of the ground beetle Chlaenius costiger in the Tsinling-Dabashan Mountains of central China
Authors: L.-N. Su, X.-C. Li, H.-Z. Meng, X.-Y. Gao, H. Yin and K. Li
Population genetic structure and demographic history of the ground beetle Chlaenius costiger (Coleoptera: Carabidae) in the Tsinling-Dabashan Mountains of central China were estimated using mitochondrial DNA sequences (Cox1-tRNALeu-Cox2) of 144 individuals from 16 local populations. The high haplotype diversity was accompanied by low nucleotide diversity... Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.7
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.7
A simplified genomic DNA extraction protocol for pre-germination genotyping in rice
Authors: Y.B. Duan, F.L. Zhao, H.D. Chen, H. Li, D.H. Ni, P.C. Wei, W. Sheng, J.T. Teng, A.M. Zhang and J.P. Xue
Genotyping is a critical step for molecular markerassisted selection in rice. Rice genomic DNA samples for genotyping are typically isolated from living tissues such as seedlings. This requires the germination of all candidate seeds and extraction of DNA from the seedlings. Currently, an ideal individual is selected from a very large number of plants, whi.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.11.12
- DOI:
- 10.4238/2015.June.11.12
DNMT3A -448A>G polymorphism and cancer risk: a meta-analysis
Authors: C.H. Liu,T. Tao1,L. Jiang1,B. Xu,L. Zhang, K. Lu,X.W. Zhang,S.Q. Chen,D.C. Liu and M. Chen
Cancer is a major public health problem worldwide that involves complex processes and factors. For instance, methylation is important in tumorigenesis. DNA (cytosine-5)-methyltransferase 3A (DNMT3A) is the main de novo methyltransferase implicated in this process. In DNMT3A, the -448A>G polymorphism is associated with cancer; however, the results of va.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.17.14
- DOI:
- http://dx.doi.org/10.4238/2015.April.17.14
Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil
Authors: A.C. Santini, J.T. Magalh�£es, J.C.M. Cascardoâ� and R.X. Corr�ªa
Chromobacterium violaceum is a free-living Gram-negative bacillus usually found in the water and soil in tropical regions, which causes infections in humans. Chromobacteriosis is characterized by rapid dissemination and high mortality. The aim of this study was to detect the genetic variability among C. violaceum type strain A.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027955
- DOI:
- 10.4238/gmr.15027955
Simultaneous selection for cowpea (Vigna unguiculata L.) genotypes with adaptability and yield stability using mixed models
Authors: F.E. Torres, P.E. Teodoro, E.V. Rodrigues, A. Santos, A.M. Corr�ªa, G. Ceccon
The aim of this study was to select erect cowpea (Vigna unguiculata L.) genotypes simultaneously for high adaptability, stability, and yield grain in Mato Grosso do Sul, Brazil using mixed models. We conducted six trials of different cowpea genotypes in 2005 and 2006 in Aquidauana, Chapadão do Sul, Dourados, and Primave.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028272
- DOI:
- 10.4238/gmr.15028272
Molecular characterization of the myosatin gene and the effect of fasting on its expression in Chinese perch (Siniperca chuatsi)
Authors: L. Liu, Y.L. Li, S.D. Xu, K.Z. Wang, P. Wu, W.Y. Chu and X.Q. Wang
Myostatin (MSTN) is an important member of the transforming growth factor-β (TGF-β) superfamily and is a muscle growth inhibitor. In the present study, we cloned the Chinese perch MSTN cDNA sequence and analyzed its expression patterns under various conditions. The MSTN full cDNA sequence was 3347 bp long, including .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028354
- DOI:
- 10.4238/gmr.15028354
IDH1 and IDH2 mutations in different histologic subtypes and WHO grading gliomas in a sample from Northern Brazil
Authors: I.A. Pess�´a, F.E.S. Sagica, N.P. Anselmo, J.R.N. Brito an E.H.C. de Oliveira
Glioma is a term used to describe tumors derived from glial cells. These tumors are divided into subgroups based on the histological morphology and similarity of their differentiated glia cells. Traditionally, they are classified according to the World Health Organization and include astrocytomas, oligodendrogliomas, ependymomas, and oligoastrocytomas. Li.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.12.6
- DOI:
- 10.4238/2015.June.12.6