Dna
Molecular identification and genetic variation of varieties of Styphnolobium japonicum (Fabaceae) using SRAP markers
Authors: R.X. Sun, C.H. Zhang, Y.Q. Zheng, Y.C. Zong, X.D. Yu and P. Huang
Thirty-four Styphnolobium japonicum varieties were analyzed using sequence-related amplified polymorphism (SRAP) markers, to investigate genetic variation and test the effectiveness of SRAP markers in DNA fingerprint establishment. Twelve primer pairs were selected from 120 primer combinations for their reproducibility and hig.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027837
- DOI:
- 10.4238/gmr.15027837
Ancient DNA in historical parchments - identifying a procedure for extraction and amplification of genetic material
Authors: T. Lech
Historical parchments in the form of documents, manuscripts, books, or letters, make up a large portion of cultural heritage collections. Their priceless historical value is associated with not only their content, but also the information hidden in the DNA deposited on them. Analyses of ancient DNA (aDNA) retrieved from parchm.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028661
- DOI:
- 10.4238/gmr.15028661
Roles of oxidative DNA damage of bone marrow hematopoietic cells in steroid-induced avascular necrosis of femoral head
Authors: Z.Q. Zhao, R. Bai, W.L. Liu, W. Feng, A.Q. Zhao, Y. Wang, W.X. Wang, L. Sun, L.S. Wu and S.X. Cui
An animal model of steroid-induced avascular necrosis of femoral head (SANFH) was established to investigate the role of oxidative DNA damage of bone marrow hematopoietic cells in SANFH. Forty-five-month-old Japanese white rabbits (male or female, 2.5 ± 0.5 kg) were randomly divided into groups A (methylprednisolone + E.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017706
- DOI:
- 10.4238/gmr.15017706
Molecular docking of the anticancer bioactive compound proceraside with macromolecules involved in the cell cycle and DNA replication
Authors: A.B. Gurung, M.A. Ali, A. Bhattacharjee, M. AbulFarah, F. Al-Hemaid, F.M. Abou-Tarboush, K.M. Al-Anazi, F.S.M. Al-Anazi and J. Lee
The bioactive compounds proceraside A, frugoside and calotropin, which were extracted from the root bark of Calotropis procera (Aiton) W.T. Aiton (family Asclepiadaceae), were recently reported to inhibit the growth of inhibition against various human cancer cell lines in vitro. However, their modes of action have not been cle.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027829
- DOI:
- 10.4238/gmr.15027829
Phylogenetic relationships among Octopodidae species in coastal waters of China inferred from two mitochondrial DNA gene sequences
Authors: Z.M. L�¼, W.T. Cui, L.Q. Liu, H.M. Li and C.W. Wu
Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a “catch-all” genus. The monophyly of octopus species in China’s coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China’s coastal waters using nucleotide sequences.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.19.7
- DOI:
- 10.4238/2013.September.19.7
Genomic DNA isolation of Acrocomia aculeata (Arecaceae) from leaf and stipe tissue samples for PCR analysis
Authors: E.C.M. Lanes, C. Nick, K.N. Kuki, R.D. Freitas and S.Y. Motoike
Macaw palm, Acrocomia aculeata is an oleaginous species of the Arecaceae family; it has been identified as one of the most promising plants for sustainable production of renewable energy, especially biodiesel. We developed an efficient protocol of genomic DNA extraction for A. aculeata using leaf and stipe tissues, based on the cationic hexadecyltrimethyl.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.9
- DOI:
- 10.4238/2013.September.23.9
Molecular evolution and adaptation of the mitochondrial cytochrome b gene in the subgenus Martes
Authors: B. Li, B. Malyarchuk, X.B. He and M. Derenko
Martes species represent a typical example of rapid evolutionary radiation and a recent speciation event. To identify regions of the genome that experienced adaptive evolution, which might provide clues to their functional importance and may be informative about the features that make each species unique, we sought evidence of molecular adaptation in the .. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.13
- DOI:
- 10.4238/2013.September.23.13
Initial assessment of natural diversity in Mexican fig landraces
Authors: M.T. GarcÃ?Âa-Ruiz, V.M. Mendoza-Castillo, E. Valadez-Moctezuma and A. Muratalla-LÃ?ºa
The common fig (Ficus carica L.) was introduced into Mexico by Spanish Franciscan missionaries in the 16th century. It is widely assumed that Mexican figs are the Spanish cultivar Black Mission. We collected and propagated 12 fig plants from six landraces from different states in Central Mexico that represent different climate. All of them were grown in a.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.23.12
- DOI:
- 10.4238/2013.September.23.12
Molecular characterization of endophytes isolated from Saccharum spp based on esterase and ribosomal DNA (ITS1-5.8S-ITS2) analyses
Authors: A.C. Leme, M.R.R. Bevilaqua, S.A. Rhoden, C.A. Mangolin, M.F.P.S. Machado and J.A. Pamphile
This study used esterases and ribosomal DNA (rDNA) markers to determine endophytic variability in order to better understand endophyte-host interactions. Polyacrylamide gel electrophoresis and esterase isoenzymes (EST; EC 3.1.1.3), with a-naphthyl acetate and β-naphthyl acetate as substrates, were used to assess relationships among endophytes. ITS1-5.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.11
- DOI:
- 10.4238/2013.September.27.11
Fast protocol for extraction of DNA from Prosopis spp leaves (plant adapted to arid environment) without liquid nitrogen
Authors: C.Y. Michel-L�³pez, D. Gonz�¡lez-Mendoza and O. Grimaldo-Juarez
The extraction of high-quality genomic DNA from Prosopis spp for polymerase chain reaction (PCR) amplification is complicated, owing to the presence of a high percentage of secondary metabolites that bind to or co-precipitate with nucleic acids. In the present study, we report a modified sodium dodecyl sulfate/phenol protocol that eliminates the use of li.. Read More»
- Genet. Mol. Res. 12(3):
- 2013.September.27.10
- DOI:
- 10.4238/2013.September.27.10