Dna
Phylogenetic relationships of twenty Gymnothorax species based on cytochrome b sequence data
Authors: M. Du, S.W. Yin and B.Z. Niu
To study the phylogenetic relationships of the genus Gymnothorax (moray eels) distributed in South China Sea, polymerase chain reactions were performed, and the amplification products were sequenced by cloning into the PMD18T-vector (TaKaRa). The entire gene sequences encoding cytochrome b (1140 bp) for 16 Gymnothorax (G. flav.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15028157
- DOI:
- 10.4238/gmr.15028157
S-adenosylmethionine, a methyl donor, up regulates tissue inhibitor of metalloproteinase-2 in colorectal cancer
Authors: Z. Hussain, M.I. Khan, M. Shahid and F.N. Almajhdi
DNA methylation is a fundamental epigenetic mechanism in regulating the expression of genes controlling crucial cell functions in cancer development. Gene silencing via CpG island methylation/demethylation in the promoter region is one of the mechanisms by which different genes are inactivated/activated in human cancers. Tissue inhibitor of metalloprotein.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.6
- DOI:
- 10.4238/2013.April.10.6
DNA methylation involved in proline accumulation in response to osmotic stress in rice (Oryza sativa)
Authors: C.Y. Zhang, N.N. Wang, Y.H. Zhang, Q.Z. Feng, C.W. Yang and B. Liu
Proline accumulation is involved in plant osmotic stress tolerance. Given that DNA methylation is related to almost all metabolic processes through regulation of gene expression, we suspected that this epigenetic modification and proline biosynthesis are probably related. To test this, we investigated whether osmotic stress-induced proline accumulation is.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.17.5
- DOI:
- 10.4238/2013.April.17.5
Genetic characterization of Fusarium graminearum and F. culmorum isolates from Turkey by using random-amplified polymorphic DNA
Authors: E. Y�¶r�¼k and G. Albayrak
Five Fusarium graminearum and 12 F. culmorum isolates, primarily pathogenic species of Fusarium head blight, were obtained from naturally infected wheat from various agro-ecological regions of Turkey. Genotyping of the isolates was carried out using random-amplified polymorphic DNA (RAPD). Sixty-five 10-mer oligonucleotide primers were used to amplify the.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.7
- DOI:
- 10.4238/2013.April.25.7
Karyotype characterization reveals active 45S rDNA sites located on chromosome termini in Smilax rufescens (Smilacaceae)
Authors: D. Pizzaia, V.M. Oliveira, A.R. Martins, B. Appezzato-da-Gl�³ria, E. Forni-Martins and M.L.R. Aguiar-Perecin
The genus Smilax (Smilacaceae) includes species of medicinal interest; consequently, their identification is important for the control of raw material used in the manufacture of phytotherapeutic products. We investigated the karyotype of Smilax rufescens in order to look for patterns that would be useful for comparative studies of this genus. To accomplis.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.1
- DOI:
- 10.4238/2013.April.25.1
Target replacement strategy for selection of DNA aptamers against the Fc region of mouse IgG
Authors: J. Ma, M.G. Wang, A.H. Mao, J.Y. Zeng, Y.Q. Liu, X.Q. Wang, J. Ma, Y.J. Tian, N. Ma, N. Yang, L. Wang and S.Q. Liao
Aptamers that recognize the IgG Fc region are of great interest because of their wide application as an immunology probing tool, for diagnostics, and as affinity agents for antibody purification. We developed a target replacement strategy as a modification of conventional Systematic Evolution of Ligands by EXponential enrichment (SELEX) in order to effici.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.25.11
- DOI:
- 10.4238/2013.April.25.11
Absence of the BaeR protein leads to the early initiation of DNA replication in Escherichia coli
Authors: Y. Yao, Wunier and Morigen
Escherichia coli cells have dozens of two-component systems to sense and respond to various stimuli, and thereby cope with changing environments. BaeS/BaeR is one such two-component system, and it deals with a variety of envelope stresses. Interestingly, the ArcA/ArcB and TorS/TorR two-component systems are known to be associated with initiation of DNA re.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.14.16
- DOI:
- 10.4238/2015.December.14.16
Selected representative microsatellite loci for genetic monitoring and population structure analysis of miniature swine
Authors: C. Wang,L.L. Xu,Y.H. Wu,X.Y. Du,X.Y. Huo,J. Gao,Z.K. Li,Y.C. Li and Z.W. Chen
To optimize the combination of microsatellite loci for genetic control of outbred swine stocks, 32 of 100 loci distributed among almost all chromosomes (except 12) were screened out by 1.5% agarose, 8% polyacrylamide gel and capillary electrophoresis scanning among 3 miniature swine outbred stocks, namely Bama (BM), Guizhou (GZ) and Tibeta (TB). The mean .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.5
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.5
CpG island evolution in the mammalian DHRS4 gene cluster and its role in the regulation of gene transcription
Authors: Z. Su, G. Liu, X. Song, B. Liang, X. Chang and D. Huang
The dehydrogenase/reductase (SDR family) member 4 (DHRS4) gene is copied during mammalian evolution; therefore, while only one DHRS4 gene is expressed in the mouse genome, the gene cluster consists of two (DHRS4 and DHRS4L1) and three (DHRS4, DHRS4L2, and DHRS4L1) copies in chimpanzees and humans, respectively. In this study, .. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027752
- DOI:
- 10.4238/gmr.15027752
Diversity and structure of a bacterial community in grassland soils disturbed by sheep grazing, in the Loess Plateau of northwestern China
Authors: Y.N. Gou, Z.B. Nan and F.J. Hou
The relationship between disturbance, biodiversity, and ecosystem function has been a hot topic recently in international ecological research, and a universally applicable model remains elusive. In this study, we assessed the diversity and structure of a bacterial community in grassland soils along a disturbance gradient due to sheep grazing. Bacteria wer.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.15.5
- DOI:
- 10.4238/2015.December.15.5