Dna
Correlation between sperm ultrastructure in infertile patients with abnormal sperm morphology and DNA damage
Authors: M. He and L. Tan
This study explored the correlation between sperm ultrastructure in infertile patients with abnormal sperm morphology and DNA damage. Three unusual sperm morphologies were selected for the experimental group namely case 1 (95% headless sperm), case 2 (98% headless sperm), and case 3 (100% headless sperm), and the control group consisted of 2 subjects (20 .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.15.6
- DOI:
- 10.4238/2015.December.15.6
Rapid discrimination between four seagrass species using hybrid analysis
Authors: M. Osathanunkul,P. Madesis,S. Ounjai,C. Suwannapoom and A. Jampeetong
Biological species are traditionally identified based on their morphological features and the correct identification of species is critical in biological studies. However, some plant types, such as seagrass, are taxonomically problematic and difficult to identify. Furthermore, closely related seagrass species, such as Halophila spp, form a taxonomically u.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.10
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.10
Cytoplasmic polymorphism and evolutionary history of plum cultivars: Insights from chloroplast DNA sequence variation of trnL-trnF spacer and aggregated trnL intron & trnL-trnF spacer
Authors: S.B. Mustapha,H. Ben Tamarzizt,G. Baraket,D. Abdallah and A. Salhi-Hannachi
We screened for polymorphisms of the non-coding region of plastid DNA in plum trees. Sequencing data from the trnL-trnF chloroplast region were used to reveal a pattern of diversity, establish phylogenetic relationships, and test the selection pressure or evolutionary demography scenario for plastome DNA. The size of the non-coding regions varied from 398.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.11
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.11
Study of genetic variation of eggplant cultivars by using RAPD-PCR molecular markers and the relationship with Phomopsis blight disease reaction
Authors: H.A. Asad, M.B. Meah, S.N. Begum, M.I. Khalil, M.Y. Rafii and M.A. Latif
Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infection ran.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.1
- DOI:
- 10.4238/2015.December.16.1
Cytogenetic description of Ancistrus abilhoai (Siluriformes: Loricariidae) from Igua�§u River basin, southern Brazil
Authors: M.O. Ribeiro,R.B. Noleto,C.A. Lorscheider,F.E. Porto,A.C. Prizon,C.H. Zawadzki,L.C. Oliveira and A.L.B. Portela Castro
The Iguaçu River basin is a tributary to the upper Paraná River in southern Brazil, and is considered an important aquatic ecoregion that, although having few species of fish, 51-71% of these are apparently endemic. Ancistrus abilhoai is one of three recently described species for this basin and is currently considered endemic to the basin. .. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.20
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.20
Identification and isolation of full-length cDNA sequences by sequencing and analysis of expressed sequence tags from guarana (Paullinia cupana)
Authors: L.C. Figueir�ªdo, A.C. Faria-Campos, S. Astolfi-Filho and J.L. Azevedo
The current intense production of biological data, generated by sequencing techniques, has created an ever-growing volume of unanalyzed data. We reevaluated data produced by the guarana (Paullinia cupana) transcriptome sequencing project to identify cDNA clones with complete coding sequences (full-length clones) and complete s.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1124
- DOI:
- 10.4238/vol10-2gmr1124
A SCAR marker for the analysis of chloroplast DNA from different cultivars of Cornus officinalis
Authors: L. Zhang, R.X. Liu, J. Wang, T. Zhang, J. Li, J.H. Shi, B.Y. Kang and S.Q. Chen
The aims of this study were to establish a random amplified polymorphic DNA (RAPD) fingerprint database of chloroplast DNA (cpDNA) from different cultivars of Cornus officinalis and to convert RAPD markers to sequence characterized amplified regions (SCAR) markers. A method of extraction was established that was suitable for obtaining cpDNA from samples r.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.16.17
- DOI:
- 10.4238/2015.December.16.17
Utilization of molecular markers for the conservation of blood cockles, Anadara granosa (Arcidae)
Authors: S.Y. Chee , M.N.S. Azizah and M.N. Devakie
We examined genetic variation in blood cockles in an effort to obtain information useful for the sustainability, management, and the stability of this species as a major commodity in the fisheries sector. Ten populations of cockles were sampled from the north to the south of the west coast of peninsular Malaysia. The cockles w.. Read More»
- Genet. Mol. Res. 10(2):
- vol10-2gmr1103
- DOI:
- 10.4238/vol10-2gmr1103
Analysis of the beta-globin gene in DNA of suspected thalassemic great apes
Authors: J. Vuch, M.S. Siori, M.P. Bigatti, L. Segat, G. De Fabrizio and S. Crovella
DNA was recovered from teeth of 2 great ape skeletons, Pan troglodytes (Ptr) and Pongo pygmaeus (Ppy), belonging to a 19th-century zoological collection. The skeletons presented morphological alterations possibly associated with β-thalassemia: Ptr had deformation of the calvaria and oro-maxillo-facial bones with porotic hyperostosis and extend.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.May.21.4
- DOI:
- 10.4238/2013.May.21.4
Ability of HMGB1 protein to bind to intrinsically bent and non-bent DNA sites in the AMPD2 gene amplicon
Authors: K.J.R. Passos, A. Fiorini, F.R. Rosado, D.V.B. Freitas, Q.A. Lima Neto, J.R. Pattaro Junior, V.P. Gaspar and M.A. Fernandez
HMGB-like proteins are architectural chromatin factors, and their function is heavily dependent on their ability to interact with DNA (especially non-canonical DNA structures). HMGB1 is involved in many DNA processes, and dysregulation of HMGB protein expression has profound effects on cellular transcription, resulting in seve.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027441
- DOI:
- 10.4238/gmr.15027441