Dna
Differential gene expression in drought-tolerant sugarcane roots
Authors: J.S. Vantini, G.C. Dedemo, D.F. Jovino Gimenez, L.F.S. Fonseca, R.I.D. Tezza, M.A. Mutton, J.A. Ferro and M.I.T. Ferro
Drought is one of the most frequent abiotic stresses limiting the productivity and geographical distribution of sugarcane culture. The use of drought-tolerant genotypes is one approach for overcoming the effects of water stress. We conducted a comparative study to identify gene expression profiles under water stress in tolerant sugarcane roots. Two differ.. Read More»
- Genet. Mol. Res. 14(2):
- 2015.June.29.13
- DOI:
- 10.4238/2015.June.29.13
Species-specific AFLP markers for identification of Zingiber officinale, Z. montanum and Z. zerumbet (Zingiberaceae)
Authors: S. Ghosh, P.B. Majumder and S. Sen Mandi
The Zingiber genus, which includes the herbs known as gingers, commonly used in cooking, is well known for its medicinal properties, as described in the Indian pharmacopoeia. Different members of this genus, although somewhat similar in morphology, differ widely in their pharmacological and therapeutic properties. The most imp.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1154
- DOI:
- 10.4238/vol10-1gmr1154
Isolation of genomic DNA from defatted oil seed residue of rapeseed (Brassica napus)
Authors: M. Sadia, M.A. Rabban, S. Hameed, S.R. Pearce and S.A. Malik
A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically followed by processing of the residual bioma.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1009
- DOI:
- 10.4238/vol10-1gmr1009
Leaf age affects the quality of DNA extracted from Dimorphandra mollis (Fabaceae), a tropical tree species from the Cerrado region of Brazil
Authors: P.A. Moreira and D.A. Oliveira
Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to ha.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1030
- DOI:
- 10.4238/vol10-1gmr1030
Genetic diversity of the honeybee Apis cerana in Yunnan, China, based on mitochondrial DNA
Authors: L. Yin and T. Ji
DNA sequence diversity in the tRNAleu-COII portion of the mitochondrial genome was investigated in samples of Apis cerana from Yunnan, China. A fragment of about 480 bp in tRNAleu-COII, including a noncoding area and part of COII, was sequenced. The noncoding area was 97-98 bp; 8 haplotypes were found, among which 5 had been reported previously, while 3 w.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.June.20.1
- DOI:
- 10.4238/2013.June.20.1
Development and evaluation of a loop-mediated isothermal amplification assay for detection of Ehrlichia canis DNA in naturally infected dogs using the p30 gene
Authors: V.C. Pinhanelli, P.N.M. Costa, G. Silva, D.M. Aguiar, C.M.L. Silva, A.L. Fachin and M. Marins
Canine monocytic ehrlichiosis (CME) is a common tick-borne disease caused by the rickettsial bacterium Ehrlichia canis (Rickettsiales: Anaplasmataceae). In view of the different stages and variable clinical signs of CME, which can overlap with those of other infections, a conclusive diagnosis can more readily be obtained by combining clinical and hematolo.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.13
- DOI:
- 10.4238/2015.December.22.13
Isolation and analysis of cell-free fetal DNA from maternal peripheral blood in Chinese women
Authors: W.C. Yang, L. Zhu, Y.M. Qiu, B.X. Zhou, J.L. Cheng, C.L. Wei, H.C. Chen, L.Y. Li, X.D. Fu and J.J. Fu
Non-invasive prenatal diagnosis is used to detect the genetic material of the fetus by isolating the cell-free fetal DNA (cffDNA) from maternal peripheral blood. In order to establish an isolation method for cffDNA from maternal peripheral blood in Chinese women, the cffDNA was acquired with a two-step centrifugation using a QlAamp DNA Blood mini kit. The.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.22.34
- DOI:
- 10.4238/2015.December.22.34
A minimal DNA cassette as a vector for genetic transformation of soybean (Glycine max)
Authors: G.R. Vianna, F.J.L. Arag�£o and E.L. Rech
Currently, the market demands products committed to protecting human health and the environment, known as clean products. We developed a protocol using DNA fragments containing only the gene sequence of interest, to replace the circular vectors containing genes for antibiotic resistance and other undesirable sequences, for obt.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1058
- DOI:
- 10.4238/vol10-1gmr1058
Genetic diversity and differentiation in Prunus species (Rosaceae) using chloroplast and mitochondrial DNA CAPS markers
Authors: S. Ben Mustapha,H. Ben Tamarzizt,G. Baraket,D. Abdallah and A. Salhi Hannachi
Chloroplast (cpDNA) and mitochondrial DNA (mtDNA) were analyzed to establish genetic relationships among Tunisian plum cultivars using the polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique. Two mtDNA regions (nad 1 b/c and nad 4 1/2) and a cpDNA region (trnL-trnF) were amplified and digested using restriction enzymes... Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.27.33
- DOI:
- http://dx.doi.org/10.4238/2015.April.27.33
A simplified universal genomic DNA extraction protocol suitable for PCR
Authors: T.Y. Wang, L. Wang, J.H. Zhang and W.H. Dong
Conventional genomic DNA extraction protocols need expensive and hazardous reagents for decontamination of phenolic compounds from the extracts and are only suited for certain types of tissue. We developed a simple, time-saving and cost-efficient method for genomic DNA extraction from various types of organisms, using relative.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr1055
- DOI:
- 10.4238/vol10-1gmr1055