Dna
Effect of inhibition of MEK pathway on 5-aza-deoxycytidine-suppressed pancreatic cancer cell proliferation
Authors: X. Wang1, H. Wang1, N. Jiang1, W. Lu1, X.F. Zhang1 and J.Y. Fang2
Pancreatic adenocarcinoma is a lethal disease because it is inoperable at the time of diagnosis. Therefore, the search for new therapeutic approaches is critical. The abnormal expression of the mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK) pathway and alteration in epigenetic modification (DNA methylation and acetylation of .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.6
- DOI:
- 10.4238/2013.November.18.6
Identification of the isoamylase 3 gene in common wheat and its expression profile during the grain-filling period
Authors: G.Z. Kang, G.Q. Liu, W. Xu, Y.J. Zhu, C.Y. Wang, H.Q. Ling and T.C. Guo
In higher plants, isoamylase-type starch debranching enzyme catalyzes the α-1,6-glucosidic linkages of glycogen and phytoglycogen. We cloned an isoamylase-type starch debranching enzyme ISA3 cDNA sequence (2883 bp), designated as TaISA3, from common wheat (Triticum aestivum), using the rapid amplification of cDNA ends method. The open reading frame .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.17
- DOI:
- 10.4238/2013.February.28.17
Universal primers to amplify the complete mitochondrial 12S rRNA gene in marine fish species
Authors: X.X. Jin, S.L. Zhao, R.X. Wang
A pair of new universal 12S mitochondrial rRNA gene primers was designed through multiple alignment analysis of the mitochondrial tRNAPhe and the 5’ region of 16S mitochondrial rRNA genes of different kinds of fishes. The primers were successfully used to amplify an expected product fragment of about 1.2 kb from various marine fish species, and the .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.15.6
- DOI:
- 10.4238/2013.October.15.6
Genetic diversity of Cosmos species revealed by RAPD and ISSR markers
Authors: A. RodrÃ?Âguez-Bernal, J.L. PiÃ?±a-Escutia, L.M. VÃ?¡zquez-GarcÃ?Âa and A.M. Arzate-FernÃ?¡ndez
The genus Cosmos is native of America and is constituted by 34 species; 28 of them are endemic of Mexico. The cosmos are used as a nematicide, antimalarial, and antioxidative agent. The aim of this study was to estimate the genetic diversity among 7 cosmos species based on random amplified polymorphic DNA (RAPD) and inter-simple sequences repeats (ISSR) m.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.13
- DOI:
- 10.4238/2013.December.4.13
Genetic diversity and population structure of different varieties of Morada Nova hair sheep from Brazil
Authors: J.S.B. Ferreira, S.R. Paiva, E.C. Silva, C.M. McManus, A.R. Caetano, D.A.E. Fa�§anha and M.A.N. de Sousa
The aim of this study was to analyze genetic diversity and population structure among varieties of White (N = 40), Red (N = 32), and Black (N = 31) Morada Nova hair sheep from flocks in the northeastern Brazilian semiarid region. Fifteen nuclear microsatellite markers and two regions of mitochondrial DNA were used. The intra-p.. Read More»
- Genet. Mol. Res. 13(2):
- 2014.April.3.20
- DOI:
- 10.4238/2014.April.3.20
Aberrant promoter methylation of the CD4 gene in peripheral blood cells of mastitic dairy cows
Authors: X.S. Wang, Y. Zhang, Y.H. He, P.P. Ma, L.J. Fan, Y.C. Wang,Y.I. Zhang, D.X. Sun, S.L. Zhang, C.D. Wang, J.Z. Song and Y. Yu
Bovine mastitis is the most common and costly disease of dairy cattle. Cluster of differentiation 4 (CD4) is closely related to the immune response in mastitis. We quantified promoter CpG methylation levels of the CD4 gene in Chinese Holsteins with clinical mastitis (CM) and in healthy controls; these levels were quantitatively detected with bisulfite pyr.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.December.4.10
- DOI:
- 10.4238/2013.December.4.10
Usefulness of cpDNA markers for phylogenetic and phylogeographic analyses of closely related cactus species
Authors: I.A.S. Bonatelli, D.C. Zappi, N.P. Taylor and E.M. Moraes
Although plastid DNA has been widely explored as a marker of choice for phylogeny and phylogeography studies, little is known about its utility for examining relationships between closely related species. The slow evolutionary rates inherent to chloroplast (cp) DNA make it difficult to perform lower level taxonomic analyses, particularly at the population.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.27
- DOI:
- 10.4238/2013.February.28.27
Molecular phylogenetic relationships among members of the family Phytolaccaceae sensu lato inferred from internal transcribed spacer sequences of nuclear ribosomal DNA
Authors: J. Lee, S.Y. Kim, S.H. Park and M.A. Ali
The phylogeny of a phylogenetically poorly known family, Phytolaccaceae sensu lato (s.l.), was constructed for resolving conflicts concerning taxonomic delimitations. Cladistic analyses were made based on 44 sequences of the internal transcribed spacer of nuclear ribosomal DNA from 11 families (Aizoaceae, Basellaceae, Didiereaceae, Molluginaceae, Nyctagin.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.February.28.15
- DOI:
- 10.4238/2013.February.28.15
Analysis of mitochondrial DNA using amplified fragment length polymorphism markers of isonuclear allocytoplasmic male sterile wheat accessions and their maintainer lines
Authors: M. Ejaz*, Z. Qidi*, Z. Gaisheng, W. Qunzhu and Z. Xinbo
To produce a good F1 hybrid variety wheat crop, it is necessary to explore novel cytoplasmic male sterility (CMS) lines and their maintainer line. This study aimed to identify cytoplasmic variation in three isonuclear-alloplasmic male sterile lines Aegilops kotschyi (Ae.kots) -90-110, Aegilops ventricosa (Ae.ven) -90-110, and Triticum spelta (T.spelta) -9.. Read More»
- Genet. Mol. Res. 12(4):
- 2013.October.30.5
- DOI:
- 10.4238/2013.October.30.5
RAPD and barcode analyses of groupers of the genus Epinephelus
Authors: K. Noikotr1, A. Chaveerach1, K. Pinthong2, A. Tanomtong1, R. Sudmoon1 and T. Tanee3
The diversity of Epinephelus species was investigated throughout Thailand. Random amplified polymorphic DNA successfully produced 1300 bands that were phylogenetically informative and used to construct cladograms. Values of pairwise genetic similarity (S) within species ranged from 0.65 in E. erythrurus to 0.99 in E. malabaricus. The interspecific values .. Read More»
- Genet. Mol. Res. 12(4):
- 2013.November.18.21
- DOI:
- 10.4238/2013.November.18.21