Dna
Mitochondrial DNA diversity of feral pigs from Karukinka Natural Park, Tierra del Fuego Island, Chile
Authors: P. Aravena,O. Skewes and N. Gouin
Control or eradication of exotic species is one of the greatest challenges facing biodiversity and ecosystem conservation. Domestic pigs (Sus scrofa domestica) were released and became feral in the southern region of Chilean Tierra del Fuego Island in the 1900s. Currently, they inhabit part of Karukinka Natural Park, an area of global conservation concern.. Read More»
- Genet. Mol. Res. 14(2):
- http://dx.doi.org/2015.April.28.6
- DOI:
- http://dx.doi.org/10.4238/2015.April.28.6
Differential structure of the intronic promoter of the Bombyx mori A3 actin gene correlated with silkworm sensitivity/resistance to nucleopolyhedrovirus
Authors: J. Tavares, J.P. Bravo, F. Gimenes, Q.A.L. Neto, A. Fiorini and M.A. Fernandez
Previous reports demonstrated that actin is necessary for nucleocapsid transport and viral gene expression during nucleopolyhedrovirus infection of Bombyx mori. The first intron of B. mori A3 actin contains a cryptic promoter that drives expression of a rare isoform. We detected differences in the size and nucleotide compositi.. Read More»
- Genet. Mol. Res. 10(1):
- vol10-1gmr978
- DOI:
- 10.4238/vol10-1gmr978
Determination of genetic variation among chickpea genotypes and their F1 crosses with RAPD markers
Authors: K. Mahmood, W. Nazeer, M. Naeem, S. Freed, M.A. Khan, G. Abbas, M. Iqbal, A.R. Kulachi, S.A. Sajid, M.A. Sadiq and M. Saleem
The intensity of genetic diversity amongst chickpea genotypes and their crosses is unknown. The current study investigated the genetic diversity of chickpea genotypes and their F1 crosses by using random amplified polymorphic DNA (RAPD) markers. We assessed the variation among 6 chickpea genotypes and 15 F1 crosses with the RAPD markers. The 6 parents and.. Read More»
- Genet. Mol. Res. 12(2):
- 2013.April.10.1
- DOI:
- 10.4238/2013.April.10.1
Segregation analysis of microsatellite (SSR) markers in sugarcane polyploids
Authors: X. Lu, H. Zhou, Y.-B. Pan, C.Y. Chen, J.R. Zhu, P.H. Chen, Y.-R. Li, Q. Cai and R.K. Chen
No information is available on segregation analysis of DNA markers involving both pollen and self-progeny. Therefore, we used capillary electrophoresis- and fluorescence-based DNA fingerprinting together with single pollen collection and polymerase chain reaction (PCR) to investigate simple sequence repeat (SSR) marker segregation among 964 single pollens.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.26
- DOI:
- 10.4238/2015.December.23.26
DGGE and multivariate analysis of a yeast community in spontaneous cocoa fermentation process
Authors: A.C.R. Ferreira, E.L.S. Marques, J.C.T. Dias and R.P. Rezende
Cocoa bean is the main raw material used in the production of chocolate. In southern Bahia, Brazil, cocoa farming and processing is an important economic activity. The fermentation of cocoa is the processing stage that yields important chocolate flavor precursors and complex microbial involvement is essential for this process. In this study, PCR-denaturin.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.34
- DOI:
- 10.4238/2015.December.23.34
Molecular identification of the genus Thryssa based on DNA barcoding
Authors: C.-Y. Ma, H.-Y. Ma, Y. Ni, W. Wang and L.-B. Ma
DNA barcoding is an effective method for identifying species by analyzing one or a few short standardized DNA sequences. In this study, we examined the utility of mitochondrial cytochrome oxidase subunit I (COI) sequences as a DNA barcode for the identification of six species belonging to the genus Thryssa: T. dussumieri, T. hamiltonii, T. kammalensis, T... Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.5
- DOI:
- 10.4238/2015.December.28.5
Genetic diversity of popcorn genotypes using molecular analysis
Authors: F.S. Resh, C.A. Scapim, C.A. Mangolin, M.F.P.S. Machado, A.T. do Amaral Jr., H.C.C. Ramos and M. Vivas
In this study, we analyzed dominant molecular markers to estimate the genetic divergence of 26 popcorn genotypes and evaluate whether using various dissimilarity coefficients with these dominant markers influences the results of cluster analysis. Fifteen random amplification of polymorphic DNA primers produced 157 amplified fragments, of which 65 were mon.. Read More»
- Genet. Mol. Res. 14(3):
- 2015.August.19.16
- DOI:
- 10.4238/2015.August.19.16
DNA barcoding and phylogenetic relationships of genera Picoides and Dendrocopos (Aves: Picidae)
Authors: Z.H. Huang, F.Y. Tu and X.J. Liao
Picoides and Dendrocopos are two closely related genera of woodpeckers (family Picidae), and members of these genera have long been the subjects of phylogenetic debate. Mitochondrial cytochrome c oxidase subunit I (COI) is a powerful marker for the identification and phylogenetic study of animal species. In the present study, we analyzed the COI barcodes .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.23.24
- DOI:
- 10.4238/2015.December.23.24
HVSI polymorphism indicates multiple origins of mtDNA in the Hazarewal population of Northern Pakistan
Authors: N. Akbar, H. Ahmad, M.S. Nadeem, B.E. Hemphill, K. Muhammad, W. Ahmad and M. Ilyas
Mitochondrial DNA (mtDNA) is an important tool used to explore ethnogenetics and the evolutionary history of human populations. In this study, hypervariable segment I (HVSI) from mtDNA was analyzed to establish the genetic lineage of the Hazarewal populations residing in the Mansehra and Abbottabad districts of Northern Pakist.. Read More»
- Genet. Mol. Res. 15(2):
- gmr.15027167
- DOI:
- 10.4238/gmr.15027167
Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue
Authors: L. Liu, C.L. Wang, W.Y. Peng, J. Yang, M.Q. Lan, B. Zhang, J.B. Li, Y.Y. Zhu and C.Y. Li
Powdery mildew and rust fungi are obligate parasites that cannot live without host organisms. They are difficult to culture in synthetic medium in the laboratory. Genomic DNA extraction is one of the basic molecular techniques used to study the genetic structure of populations. In this study, 2 different DNA extraction methods, Chelex-100 and cetyltrimeth.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.December.28.1
- DOI:
- 10.4238/2015.December.28.1