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Animal Genetics   Research Article

Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids

Authors: Y.D. Zhong, X.Y. Sun, E.Y. Liu, Y.Q. Li, Z. Gao and F.X. Yu

Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA.. Read More»

Genet. Mol. Res. 15(2):
Human Genetics   Research Article

Respiratory chain complex III deficiency in patients with tRNA-leu mutation

Authors: J. Jiang, X.L. Wang and Y.Y. Ma

The aim of this study was to investigate the clinical and genetic profiles of mitochondrial disease resulting from deficiencies in the respiratory chain complex III. Three patients, aged between 8 months and 12 years, were recruited for this study. The activities of mitochondrial respiratory chain complexes in the peripheral leucocytes were spectrophotome.. Read More»

Genet. Mol. Res. 14(4):
2015.December.28.12
DOI:
10.4238/2015.December.28.12
Human Genetics   Research Article

Genetic diversity among different physiological traits of Sorghum bicolor cultivars of subtropical origin

Authors: S.O. Bafeel

The genetic diversity of Saudi locally growing sorghum (Sorghum bicolor) cultivars has not been thoroughly characterized. To understand the genomic patterns of diversification in Saudi sorghum cultivars (N = 7), random amplified polymorphic DNA (RAPD) was used as a rapid, inexpensive method for providing information regarding genomic variability below the.. Read More»

Genet. Mol. Res. 14(3):
2015.August.21.3
DOI:
10.4238/2015.August.21.3
Human Genetics   Research Article

Effect of uric acid on mitochondrial function and oxidative stress in hepatocytes

Authors: Y. Yang, Y. Zhou, S. Cheng, J.L. Sun, H. Yao and L. Ma

Here, we investigated the effect of uric acid (UA) on hepatocyte mitochondria. Hepatocytes cultured in vitro were treated with varying concentrations of UA. The change in apoptotic activity was detected by flow cytometry. The DNA damage index 8-hydroxy-deoxy-guanosine (8-OHdG) and mitochondrial function indices succinate dehyd.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028644
DOI:
10.4238/gmr.15028644
Human Genetics   Research Article

Molecular identification of Achyranthis Bidentatae Radix by using DNA barcoding

Authors: D.-Y. Hou, G.-P. Wang, L.-H. Zhi, H.-W. Xu, H.-L. Liang, M.-M. Yang and G.-A. Shi

Achyranthis Bidentatae Radix has a long history in China as a commonly used herb that can be used to treat various diseases, including those related to the liver, muscles, bones, and kidneys. Recently, an increase in the number of adulterants has been reported, which affects the clinical safety of Achyranthis Bidentatae Radix... Read More»

Genet. Mol. Res. 15(2):
Human Genetics   Research Article

Relevance of sampling and DNA extraction techniques for the analysis of salivary evidence from bite marks: a case report

Authors: M.L. Ch�¡vez-Briones, R. Hern�¡ndez-Cort�©s, G. Jaramillo-Rangel and M. Ortega-Mart�­nez

Bite mark evidence has been repeatedly found in criminal cases. Physical comparison of a bite mark to the teeth of available suspects may not always be possible. Experimental studies have shown that the analysis of DNA present in the saliva recovered from bite marks might help in the identification of individuals. However, the application of this approach.. Read More»

Genet. Mol. Res. 14(3):
2015.August.21.23
DOI:
10.4238/2015.August.21.23
Medical Genetics   Research Article

Effect of DNase treatment on RNA extraction from preimplantation murine embryos

Authors: J. Norhazlin, M.N.K. Nor-Ashikin, B.P. Hoh, S.H. Sheikh Abdul Kadir, S. Norita, M. Mohd-Fazirul, W.J. Wan-Hafizah, D. Razif, M.H. Rajikin and B. Abdullah

The quality of RNA is crucial when performing microarray experiments. This is particularly important when dealing with preimplantation embryos, from which a minimum yield of RNA of good quality can be produced. We report the optimization of several RNA extraction methods applied to preimplantation embryos at different stages of development. The quality of.. Read More»

Genet. Mol. Res. 14(3):
2015.August.28.1
DOI:
10.4238/2015.August.28.1
Plant Genetics   Research Article

Soybean Stem Fly, Melanagromyza sojae (Diptera: Agromyzidae), in the New World: detection of high genetic diversity from soybean fields in Brazil

Authors: J.A. Arnemann, W.T. Tay, T.K. Walsh H. Brier, K. Gordon,F. Hickmann, G. Ugalde and J.V.C. Guedes

Soybean Stem Fly (SSF), Melanagromyza sojae (Zehntner), belongs to the family Agromyzidae and is highly polyphagous, attacking many plant species of the family Fabaceae, including soybean and other beans. SSF is regarded as one of the most important pests in soybean fields of Asia (e.g., China, India), North East Africa (e.g.,.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028610
DOI:
10.4238/gmr.15028610
Human Genetics   Research Article

Correlation between methylation of the E-Cadherin gene and malignancy of prostate cancer

Authors: S.Q. Zhang, G.Q. Zhang and L. Zhang

Prostate cancer is a common malignant tumor in males with an unclear pathogenic mechanism. As one epigenetic regulation mechanism, DNA methylation of the whole genome and specific gene(s) plays critical roles in pathogenesis, progression, diagnosis, and treatment of prostate cancer. The E-Cadherin gene is involved in cell meta.. Read More»

Genet. Mol. Res. 15(2):
gmr.15028046
DOI:
10.4238/gmr.15028046
Microbial Genetics   Research Article

Genomic characterization and sequence diversity of the �²2-microglobulin gene in the miiuy croaker, Miichthys miiuy

Authors: Y.N. Sun, X.R. Su, T.J. Xu and T.W. Li

β2-Microglobulin (β2m) is related to major histocompatibility complex class I alpha chains, and forms cell-surface glycoproteins that mediate a variety of functions in immune defense. In general, β2m has no isoforms and is not polymorphic in higher vertebrates, but polymorphisms between different alleles have been found in some fish species.. Read More»

Genet. Mol. Res. 14(3):
2015.August.28.9
DOI:
10.4238/2015.August.28.9