Dna
Comparative analysis of noncoding sequences of orthologous bovine and human gene pairs
Authors: Melissa Nunes Miziara, Penny K. Riggs and M. Elisabete J. Amaral
Genomic sequence comparison across species has enabled the elucidation of important coding and regulatory sequences encoded within DNA. Of particular interest are the noncoding regulatory sequences, which influence gene transcriptional and posttranscriptional processes. A phylogenetic footprinting strategy was employed to iden.. Read More»
- Genet. Mol. Res. 3(4):
Less Ã?Â?mtDNA4977 than normal in various types of tumors suggests that cancer cells are essentially free of this mutation
Authors: Maria Angela C. Dani, Sergio U. Dani, Silmara P.G. Lima, Alfredo Martinez, Benedito M. Rossi, Fernando Soares, Marco A. Zago and Andrew J.G. Simpson
Levels of mtDNA4977 deletions (DmtDNA4977) have been found to be lower in tumors than in adjacent non-tumoral tissues. In 87 cancer patients, DmtDNA4977 was detected by multiplex polymerase chain reaction (PCR) amplification in 43 (49%) of the tumors and in 74 (85%) of the samples of non-tumoral tissues that were adjacent to t.. Read More»
- Genet. Mol. Res. 3(3):
DNA repair in Chromobacterium violaceum
Authors: FÃ?¡bio Teixeira Duarte, FabÃ?Âola Marques de Carvalho, Uaska Bezerra e Silva, KÃ?¡tia Castanho Scortecci, Carlos Alfredo Galindo Blaha, Lucymara Fassarella Agnez-Lima, Silvia Regina, Batistuzzo de Medeiros
Chromobacterium violaceum is a Gram-negative β-proteobacterium that inhabits a variety of ecosystems in tropical and subtropical regions, including the water and banks of the Negro River in the Brazilian Amazon. This bacterium has been the subject of extensive study over the last three decades, due to its biotechnological.. Read More»
- Genet. Mol. Res. 3(1):
Effects of crude extracts of Agaricus blazei on DNA damage and on rat liver carcinogenesis induced by diethylnitrosamine
Authors: LuÃ?Âs Fernando Barbisan, Clarissa Scolastici, Maristela Miyamoto, Daisy Maria Favero Salvadori, LÃ?ºcia Regina Ribeiro, Augusto Ferreira da Eira, JoÃ?£o Lauro Viana de Camargo
The effects of crude extracts of the mushroom Agaricus blazei Murrill (Agaricaceae) on both DNA damage and placental form glutathione S-transferase (GST-P)-positive liver foci induced by diethylnitrosamine (DEN) were investigated. Six groups of adult male Wistar rats were used. For two weeks, animals of groups 3 to 6 were trea.. Read More»
- Genet. Mol. Res. 2(3):
Evaluation of a method for high yield purification of largely intact mitochondrial DNA from human placentae
Authors: Sergio U. Dani, Alessandra Cristina Gomes-Ruiz, Maria Angela C. Dani
We developed, and quantitatively and qualitatively evaluated an easily reproducible method for high yield purification of mitochondrial DNA (mtDNA) from human placentae by mechanical tissue disruption, differential centrifugation of mitochondria, enzymatic digestion, phenol extraction and ethanol precipitation. Average mtDNA y.. Read More»
- Genet. Mol. Res. 2(2):
Escherichia coli as a model system to study DNA repair genes of eukaryotic organisms
Authors: Luiz Augusto-Pinto, Carlos Gustavo Regis da Silva, D�©bora de Oliveira Lopes, Alice Machado-Silva, Carlos Renato Machado
The bacteria Escherichia coli has been widely employed in studies of eukaryotic DNA repair genes. Several eukaryotic genes have been cloned by functional complementation of mutant lineages of E. coli. We examined the similarities and differences among bacterial and eukaryotic DNA repair systems. Based on these data, we examine.. Read More»
- Genet. Mol. Res. 2(1):
CENP-B is a conserved gene among vegetal
Authors: Olga Barbosa-Cisneros and Rafael Herrera-Esparza
To explore the CENP-B centromere protein in beans, carrots, onions and potatoes, total RNA was isolated and reverse transcribed by PCR, and the cDNA encoding the CENP-B amino terminus domain amplified using CENP-B oligonucleotides. Blots containing PCR products were hybridized with a nick-translated pG/CNPB probe containing a .. Read More»
- Genet. Mol. Res. 1(3):
Frequency of the hypervariable DNA loci D18S849, D3S1744, D12S1090 and D1S80 in a mixed ancestry population of Chilean blood donors
Authors: M. Acu�±a, H. Jorquera, L. Cifuentes and L. Armanet
Blood donors (N = 150) at San José Hospital (Santiago, Chile) were typed for one VNTR locus (D1S80) and three STR loci (D18S849, D3S1744, D12S1090). A questionnaire was used to determine the socioeconomic level of the donors, because it is known that some genetic markers (e.g., the ABO and Rh groups) are differentially .. Read More»
- Genet. Mol. Res. 1(2):
Further phenotypic characterization of pso mutants of Saccharomyces cerevisiae with respect to DNA repair and response to oxidative stress
Authors: Cristina Pungartnik, Jaqueline Picada, Martin Brendel and Jo�£o A.P. Henriques
The sensitivity responses of seven pso mutants of Saccharomyces cerevisiae towards the mutagens N-nitrosodiethylamine (NDEA), 1,2:7,8-diepoxyoctane (DEO), and 8-hydroxyquinoline (8HQ) further substantiated their allocation into two distinct groups: genes PSO1 (allelic to REV3), PSO2 (SNM1), PSO4 (PRP19), and PSO5 (RAD16) const.. Read More»
- Genet. Mol. Res. 1(1):