Authors: Q. Zhang, J.M. Niu, S.B. Wu, A. Buyantuyev and J.J. Dong
Genetic diversity of Stipa breviflora populations in the Inner Mongolian grasslands of China and its possible correlation with climatic factors was examined using geographic information systems and random amplified polymorphism DNA analysis. A total of 308 bands were produced with 28 arbitrary decamer oligonucleotide. Three major findings were demonstrate.. Read More»
Authors: M. Akkurt
Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods - namely three DNA isolation protocols .. Read More»
Authors: W.H. Shi, Y.B. Yu, Z.-Y. Li, X.-C. Guo, Z.-L. Wei and Z.-H. Zheng
The purpose of this study was to screen for genes that were differentially expressed between a human gastric carcinoma cell line (HGC-27) and their tumor spheres, using the gene chip technique. The HGC-27 cells and tumor sphere cells were cultured in vitro in a sterile environment. Total RNA was extracted from both sa.. Read More»
Authors: M.C. Parra-Lobato, F.J. Delgado-Martinez and M.C. Gomez-Jimenez
A total of 91 wild olive accessions and 31 olive cultivars growing in the Extremadura region of central-western Spain were analyzed using morphological traits and RAPD markers. We focused on three main and 16 minor Spanish olive cultivars that are recognized as native or local to the Extremadura region. The five arbitrary 10-mer primers tested on the oliv.. Read More»
Authors: K.C.N. RabĂ?ÂȘlo, C.M.R. Albuquerque, V.B. Tavares, S.M. Santos, C.A. Souza, T.C. Oliveira, N.C.L. Oliveira and S. Crovella
Investigations of any type of crime invariably starts at the crime scene by collecting evidence. Thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. Hematophagous mosquitoes were collected from two residential units; saliva of.. Read More»
Authors: M.X. Sui, H.H. Wang and Z.W. Wang
The current study aimed to investigate the coding sequence, polymorphisms, and expression of the RERG gene in indigenous Chinese goats. cDNA of RERG, obtained through reverse transcription PCR was analyzed using bioinformatic techniques. Polymorphisms in the exon regions of the RERG gene were identified and their associations .. Read More»
Authors: S.A. Rhoden, A. Garcia, C.J. Rubin Filho, J.L. Azevedo and J.A. Pamphile
Various types of organisms, mainly fungi and bacteria, live within vegetal organs and tissues, without causing damage to the plant. These microorganisms, which are called endophytes, can be useful for biological control and plant growth promotion; bioactive compounds from these organisms may have medical and pharmaceutical applications. Trichilia elegans .. Read More»
Authors: J. Dai, X. Liu and Y. Wang
Rhizobia are soil bacteria with the capacity to induce nitrogen-fixing nodules on the roots or stems of legume plants. A total of 40 bacterial isolates from the root nodules of Caragana microphylla growing in desert soil in Ningxia, China, were analyzed for genetic diversity and phylogenetic position. These isolates were classified into 7 types of 16S rib.. Read More»
Authors: X.J. Zhang, P. Liu and F. Zhu
We aimed to investigate the association between genetic variants of the DNA repair genes XPG, CSB, XPC, CCNH, and MMS19L in the nucleotide excision repair (NER) pathway and risk of prostate cancer in a population in China. This study included 229 patients with newly diagnosed and histopathologically confirmed primary prostate .. Read More»
Authors: J. Zhou, Q. Liu M. Mao and Y. Tong
The ubiquitin-proteasome system (UPS) regulates many cellular processes, including protein stability, cell cycle control, DNA repair, transcription, signal transduction, and protein trafficking. In fact, UPS plays a key role in various stress conditions such as ischemia, glutamate toxicity, Alzheimer’s disease, and Parki.. Read More»