Dna
Identification and analysis of a 5-bp indel of a porcine BMP7 gene promoter
Authors: P. Lu, X. Feng, J.S. Zhou, X.B. Liu, Q.X. Chen and M. Li
Bone morphological protein7 (BMP7), a member of the transforming growth factor-β (TGF-β) family, was first identified because of its ability to induce ectopic chondro-osteogenesis in vivo. It also plays a crucial role in the growth, development, and physiological functioning of the reproductive system. Among intra-ov.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.9.19
- DOI:
- http://dx.doi.org/10.4238/2014.June.9.19
Isolation and expression analysis of the soybean GmPic gene
Authors: A.-H. Sha, H. Wu, X.-M. Fu, Q.-L. Zhang, Q.-L. Guo, Y.-H. Chen and X.-A. Zhou
The differential screening method was used to isolate the soy photoperiodic response-related genes and to further elucidate the molecular mechanisms of the soybean photoperiodic response. The light-sensitive species Zhong Dou 24 was used to receive long-time sunshine, short-time sunshine, and natural sunshine treatment. The cD.. Read More»
- Genet. Mol. Res. 13(2):
- http://dx.doi.org/2014.June.11.2
- DOI:
- http://dx.doi.org/10.4238/2014.June.11.2
Microsatellite marker-based identification and genetic relationships of olive cultivars from the Extremadura region of Spain
Authors: F.J. Delgado-Martinez, I. Amaya, J.F. S�¡nchez-Sevilla and M.C. Gomez-Jimenez
Seventy-seven olive accessions corresponding to 25 cultivars from the Extremadura region of Spain were studied using four microsatellite or SSR markers in order to fingerprint them, and evaluate genetic similarity and relationships between local and introduced olive cultivars. The number of alleles per locus ranged from 4 to 8.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.10.7
- DOI:
- 10.4238/2012.April.10.7
Molecular barcoding of venomous snakes and species-specific multiplex PCR assay to identify snake groups for which antivenom is available in Thailand
Authors: A. Supikamolseni1,2, N. Ngaoburanawit3, M. Sumontha4, L. Chanhome5,S. Suntrarachun6, S. Peyachoknagul2,7 and K. Srikulnath1,2,7
DNA barcodes of mitochondrial COI and Cytb genes were constructed from 54 specimens of 16 species for species identification. Intra- and interspecific sequence divergence of the COI gene (10 times) was greater than that of the Cytb gene (4 times), which suggests that the former gene may be a better marker than the latter for species delimitation in snakes.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.18
- DOI:
- 10.4238/2015.October.29.18
Mitochondrial DNA dynamics during in vitro culture and pluripotency induction of a bovine Rho0 cell line
Authors: L.V.F. Pess�´a1, F.F. Bressan1,2, M.R. Chiaratti1,3, P.R.L. Pires1, F. Perecin1,2,L.C. Smith1,4 and F.V. Meirelles1,2
Large number of cellular changes and diseases are related to mutations in the mitochondrial DNA copy number. Cell culture in the presence of ethidium bromide is a known way of depleting mitochondrial DNA and is a useful model for studying such conditions. Interestingly, the morphology of these depleted cells resembles that of pluripotent cells, as they pr.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.29
- DOI:
- 10.4238/2015.October.29.29
Development of novel DNA markers for genetic analysis of grey hamsters by cross-species amplification of microsatellites
Authors: C. Wang1,2*, S.J. Zhang1*, X.Y. Du1, Y.M. Xu3, X.Y. Huo1, L.F. Liao3 and Z.W. Chen1
The grey hamster has been used in biomedical research for decades. However, effective molecular methods for evaluating the genetic structure of this species are lacking, which hinders its wider usage. In this study, we employed cross-amplification of microsatellite loci of species within the same genus by polymerase chain reaction. Loci screened included .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.13.19
- DOI:
- 10.4238/2015.November.13.19
Real-time PCR evaluation of cell-free DNA subjected to various storage and shipping conditions
Authors: Q. Wang, Y. Cai, P. Brady and J.R. Vermeesch
In this study, we attempted to explore the factors affecting the yield of cell-free fetal DNA (cffDNA) obtained from maternal blood samples, including the use of different types of collection tubes, the interval between sample processing, and sample shipping under extreme weather conditions. Blood samples were drawn into K3EDT.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.19.23
- DOI:
- 10.4238/2015.October.19.23
Assessing the genotoxicities of sparteine and compounds isolated from Lupinus mexicanus and L. montanus seeds by using comet assay
Authors: M.R. Silva, C.M. Alvarez, P.M. GarcÃ?Âa and M.A. Ruiz
The genus Lupinus is widely distributed. Its seeds are used for animal and human food, and Lupinus possesses pharmacological potential because of its high content of quinolizidine alkaloids and flavonoids; however, there is little available information about its genotoxicity. We used the comet assay and staminal nuclei of Tradescantia (clone 4430) to eval.. Read More»
- Genet. Mol. Res. 13(4):
- 2014.December.12.12
- DOI:
- 10.4238/2014.December.12.12
Detection and sequence analysis of the DNA repair gene RAD51 in the Korean spider Callobius koreanus (Amaurobiidae)
Authors: J.Y. Kim and Y.C. Park
We identified a partial sequence (483 bp) of the RAD51 gene from the Korean spider Callobius koreanus. Sequence variation was found at one position during alignment with the human RAD51 gene sequence. This partial sequence included the region corresponding to exon 4 in the human RAD51 gene, which encodes 39 amino acids. These results show that the RAD51 g.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.18.28
- DOI:
- 10.4238/2015.November.18.28
Analysis of gene expression patterns and levels in maize hybrids and their parents
Authors: H.S. Nie, S.P. Li, X.H. Shan, Y. Wu, S.Z. Su, H.K. Liu, J.Y. Han and Y.P. Yuan
Heterosis has greatly contributed to conventional plant breeding and is widely used to increase crop plant productivity. However, although some studies have explored the mechanisms of heterosis at the genomic and transcriptome level, these mechanisms still remain unclear. The growth and development of maize seedlings and immat.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.November.30.17
- DOI:
- 10.4238/2015.November.30.17