Dna
Cloning and sequence analysis of the coding sequence of �²-actin cDNA from the Chinese alligator and suitable internal reference primers from the �²-actin gene
Authors: H.N. Zhu, S.Z. Zhang, Y.K. Zhou, C.L. Wang and X.B. Wu
β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequ.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.9.4
- DOI:
- 10.4238/2015.October.9.4
Mitochondrial D-loop nucleotide diversity in Astyanax (Osteichthyes, Characidae) from the upper Paran�¡ and upper Paraguay River basins
Authors: A.J. Prioli, V.A. Carlo, T.V. Soria, S.M.A.P. Prioli, C.S. Pavanelli, R.A. Prioli, V.N. Gomes, H.F. J�ºlio Jr. and L.M. Prioli
The morphological discrimination between the species Astyanax altiparanae and A. asuncionensis of the upper Paraná River and Paraguay River basins, respectively, has always been difficult. Two D-loop haplogroups of A. altiparanae are known, one with the presence (AltoPR) or the absence (AltoPR-D) of a 32-bp block simila.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.5
- DOI:
- 10.4238/2012.April.27.5
DNA isolation protocol for the medicinal plant lemon balm (Melissa officinalis, Lamiaceae)
Authors: S. Ghaffariyan, S.A. Mohammadi and S. Aharizad
Lemon balm (Melissa officinalis) is a medicinal plant that is widely used as a sedative or calmant, spasmolytic and antibacterial agent and sleep aid. This has led to a high demand for lemon balm products, resulting in the extinction of this species in some of its natural habitats. Molecular techniques have increasingly been u.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.3
- DOI:
- 10.4238/2012.April.27.3
Detection of Y chromosome microdeletions and mitochondrial DNA mutations in male infertility patients
Authors: A.I. G�¼ney, D. Javadova, D. K�±rac, K. Ulucan, G. Koc, D. Ergec, H. Tavukcu and T. Tarcan
Infertility affects about 10-15% of all couples attempting pregnancy with infertility attributed to the male partner in approximately half of the cases. Proposed causes of male infertility include sperm motility disturbances, Y chromosome microdeletions, chromosomal abnormalities, single gene mutations, and sperm mitochondrial.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.2
- DOI:
- 10.4238/2012.April.27.2
AMP-activated protein kinase regulates autophagic protection against cisplatin-induced tissue injury in the kidney
Authors: L. Wei, W. Chen, Y. Zou, H. Huang, B. Pan, S. Jin, R. Huang, S. Nie and G. Kong
Although the nephrotoxicity of cisplatin has been well documented as a major side effect of chemotherapy, the exact mechanism by which prosurvival and apoptotic pathways interplay to determine renal pathology remains elusive. Recent studies suggested that autophagy might serve as an adaptive mechanism to promote cell survival .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.13
- DOI:
- 10.4238/2015.October.5.13
Phylogenetic relationship of Turkish Apis mellifera subspecies based on sequencing of mitochondrial cytochrome C oxidase I region
Authors: F. ��zdil and F. �°lhan
Mitochondrial DNA sequence variation can be used to infer honey bee evolutionary relationships. We examined DNA sequence diversity in the cytochrome C oxidase I (COI or Cox1) gene segment of the mitochondrial genome in 112 samples of Apis mellifera from 15 different populations in Turkey. Six novel haplotypes were found for th.. Read More»
- Genet. Mol. Res. 11(2):
- 2012.April.27.12
- DOI:
- 10.4238/2012.April.27.12
Construction of a full-length cDNA library and preliminary analysis of expressed sequence tags from lymphocytes of half-pipe snowboarding athletes
Authors: Y.H. Zhao, Z.B. Zhang, C.Q. Zhao, Y. Zhang, Y.F. Wang, W.J. Guan and Z.Q. Zhu
The genes of top athletes are a valuable genetic resource for the human race, and could be exploited to identify novel genes related to sports ability, as well as other functions. We analyzed the expressed sequence tags from top half-pipe snowboarding athletes using the SMART complementary DNA (cDNA) library construction method to elucidate the characteri.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.13
- DOI:
- 10.4238/2015.October.21.13
Molecular characterization of twenty polymorphic microsatellite markers in the polyploid fruit tree species Syzygium samarangense (Myrtaceae)
Authors: J.M. Lai, C.C. Tsai, C.R. Yen, Y.Z. Ko, S.R. Chen, I.S. Weng, Y.S. Lin and Y.C. Chiang
Syzygium samarangense (Blume) Merr. & Perry (wax apple) is an important commercial fruit tree in Southeast Asia. Here, microsatellite markers were developed to evaluate genetic diversity and distinguish cultivars in this species. In total, 161 microsatellite loci with sufficient flanking sequences to design primer sets were isolated from wax apple usi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.21.22
- DOI:
- 10.4238/2015.October.21.22
Relationship of EGFR DNA methylation with the severity of non-small cell lung cancer
Authors: J. Li, X.F. Jia, J. Liu, J.J. Liu and H.B. Zhao
The aim of this study was to study the relationship of EGFR DNA methylation with the severity of non-small-cell lung cancer (NSCLC). We enrolled 54 patients with NSCLC between March 2013 and June 2014 from Department of Cardiothoracic Surgery in our hospital. The methylation levels in the promoter region of the EGFR gene in ca.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.5.5
- DOI:
- 10.4238/2015.October.5.5
Differentiation of four strains of Chinese soft-shelled turtle (Pelodiscus sinensis) based on high-resolution melting analysis of single nucleotide polymorphism sites in mitochondrial DNA
Authors: H.Q. Zhang, C. Zhang, X.J. Xu, J.J. Zhu, Z.Y. He and J.Z. Shao
The Chinese soft-shelled turtle (Pelodiscus sinensis) has been one of the most economically important aquatic animals in China for thousands of years, and several breeding strains have been formed. Since the morphological characteristics of some strains are similar, a rapid and accurate molecular method to differentiate between strains is required. In thi.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.26.10
- DOI:
- 10.4238/2015.October.26.10