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Endangered Species

Microbial Genetics   Research Article

Development of 21 novel polynucleotide microsatellite markers in a Clithon retropictum (Gastropoda: Neritidae), using next-generation sequencing

Authors: Young Se Hyun, Ha Yeun Song, Min-Seop Kim, Hye Suck An

The neritid species Clithon retropictum (Gastropoda), an oviparous snail, is distributed in freshwater and brackish water in southern Korea, south-east Japan, southern China and Taiwan. This species is listed as a class II endangered species in Korea because of its decreasing population size. It has led to an increase in the n.. Read More»

Genet. Mol. Res. 16(4):
gmr16039806
DOI:
10.4238/gmr16039806
Plant Genetics   Research Article

Reduced genetic diversity in endemic Brazilian Lymania spp (Bromeliaceae) populations and implications for conservation

Authors: V.C.C. Pampon�©t1, T.F. Alves2, R.A. Martinez3, R.X. Corr�ªa1,2 and F.A. Gaiotto1,2

We analyzed the genetic diversity of populations of two sympatric species of Lymania (Bromeliaceae), both endemic to the Atlantic rainforest of southern Bahia (Brazil). Lymania azurea has a restricted occurrence, while Lymania smithii has a wider distribution. Our aim was to provide genetic data to contribute to the design of more efficient conservation s.. Read More»

Genet. Mol. Res. 12(4):
2013.October.10.1
DOI:
10.4238/2013.October.10.1
Microbial Genetics   Research Article

Fine-scale spatial genetic structure of an endangered marsh herb, Caldesia grandis (Alismataceae)

Authors: X.-L. Yue, J.-M. Chen, Y.-H. Guo and Q.-F. Wang2

The endangered marsh herb, Caldesia grandis, is native to China. We investigated the spatial structure of the genetic variation of three populations of C. grandis using RAPD markers and spatial autocorrelation analysis, based on the method of equal distance interval. A total of 157 individuals were sampled from four patches collected from the region of Hu.. Read More»

Genet. Mol. Res. 11(3):
2012.May.18.10
DOI:
10.4238/2012.May.18.10
Plant Genetics   Research Article

Population genetic structure in Phyla scaberrima from Mexico and Colombia assessed by AFLP markers and implications for conservation

Authors: L.G. Androcioli, E.A. Ruas, L.A. Rodrigues, C.F. Ruas , H.E.R. Perilla, P.M. Ruas

Phyla scaberrima (Verbenaceae) is a herbaceous peren­nial species that is distributed from Mexico (center of origin) to Colom­bia, growing in forest and swamp edges or grasslands from sea level up to an altitude of 1800 m. The chemical properties and uses in popular medicine have drastically affected the population siz.. Read More»

Genet. Mol. Res. 14(4):
2015.December.1.21
DOI:
10.4238/2015.December.1.21
Animal Genetics   Research Article

Molecular identification of cetaceans from the West Atlantic using the E3-I5 region of COI

Authors: L.H.O. Falc�£o, A.S. Campos, J.E.P. Freitas, M.A.A. Furtado-Neto and V.V. Faria

Molecular identification is very useful in cases where morphology-based species identification is not possible. Examples for its application in cetaceans include the identification of carcasses of stranded animals in advanced state of decomposition and body parts that are illegally traded. One DNA region that is often used for.. Read More»

Genet. Mol. Res. 16(2):
gmr16029607
DOI:
10.4238/gmr16029607
Plant Genetics   Research Article

Optimization of DNA extraction from fresh leaf tissues of Melanoxylon brauna (Fabaceae)

Authors: D.B. Borges, M.B. Amorim, A.M. Waldschmidt, E. Mariano-Neto, C.V. Vivas and D.G. Pereira

Melanoxylon brauna (Fabaceae - Caesalpinioideae) is an endemic and valuable hardwood tree species in the Brazilian Atlantic rainforest; it is comparable to African ebony wood. We tested three protocols of DNA extraction based on the citrimonium bromide (CTAB) method and evaluated the quantity, purity and integrity of the DNA. .. Read More»

Genet. Mol. Res. 11(2):
2012.May.22.8
DOI:
10.4238/2012.May.22.8
Plant Genetics   Research Article

Application of 16S rRNA, cytochrome b and control region sequences for understanding the phylogenetic relationships in Oryx species

Authors: H.A. Khan, I.A. Arif, A.A. Al Homaidan and A.H. Al Farhan

The present study reports the application of mitochondrial markers for the molecular phylogeny of Oryx species, including the Arabian oryx (AO), scimitar-horned oryx (SHO) and plains oryx (PO), using the Addax as an outgroup. Sequences of three molecular markers, 16S rRNA, cytochrome b and a control region, for the above four taxa were aligned and the top.. Read More»

Genet. Mol. Res. 7(4):
vol7-4gmr514
DOI:
10.4238/vol7-4gmr514
Animal Genetics   Research Article

Isolation and characterization of microsatellite markers for the White Cloud Mountain minnow (Tanichthys albonubes) in wild and cultured populations

Authors: J. Zhao, D.-Y. Tseng, H.-D. Lin and X.-T. Lin

We developed 12 microsatellite loci for the endangered minnow species, Tanichthys albonubes, using PCR-based isolation of microsatellite arrays. These new markers were tested in 26 individuals from a wild population collected from Guangzhou in China and 26 individuals from a cultured strain. The number of alleles ranged from t.. Read More»

Genet. Mol. Res. 10(3):
vol10-3gmr1556
DOI:
10.4238/vol10-3gmr1556
Plant Genetics   Research Article

Population structure and genetic variation of the endangered species Elaeagnus mollis Diels (Elaeagnaceae)

Authors: J.M. Zhang and F. Zhang

Elaeagnus mollis Diels is a group of shrubs and dwarf trees endemic to China and are endangered plants. However, the reason why these plants are endangered remains controversial. The current study aimed to explore the endangered status of E. mollis from a genetic perspective and to propose conservation strategies for this species. Using 16 polymorphic all.. Read More»

Genet. Mol. Res. 14(2):
2015.June.1.12
DOI:
10.4238/2015.June.1.12
Animal Genetics   Research Article

Genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China revealed by simple-sequence repeat markers

Authors: Y. Zhao, Y. Li, Y. Liu and Y.F. Yang

Simple-sequence repeat (SSR) markers were employed to assess the genetic diversity of wild Prunus cerasifera Ehrhart (wild cherry plum) in China. Fourteen SSR primer pairs generated a total of 94 alleles (90 were polymorphic, accounting for 95.74%), with a mean of 6.71 alleles per locus. The number of alleles detected at each locus ranged from 2 at BPPCT .. Read More»

Genet. Mol. Res. 14(3):
2015.July.28.7
DOI:
10.4238/2015.July.28.7