Expression
Gene expressions levels of 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPase�² in gill tissue of Mugil cephalus acclimated to low salinity
Authors: L. Li, M. Jiang and X.Q. Shen
Fishes adapt to salinity changes primarily through osmotic pressure regulation, a process often associated with several genes, including 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ. The present study investigated the differential expression of genes 14-3-3a, NKCCla, APO-14, and Na+-K+-ATPaseβ in the gill tissue of Mu.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019444
- DOI:
- 10.4238/gmr16019444
Nramp1 gene expression in different tissues of Meishan piglets from newborn to weaning
Authors: C.H. Dai, J.Y. Wu, C.X. Zhao, L.H. Yu, W.B. Bao and S.L. Wu
Natural resistance-associated macrophage protein gene 1 (Nramp1) plays an important role in the innate immune response of swine, and is believed to influence disease resistance. In this study, a real-time quantitative polymerase chain reaction technique was used to investigate Nramp1 expression in 12 different tissues in newbo.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019288
- DOI:
- 10.4238/gmr16019288
Expression profiling based on coexpressed modules in obese prepubertal children
Authors: J.F. Zhang G.Y. Yao Y.H. Wu
The aim of this study was to identify related genes and the underlying molecular mechanisms in obese patients who show a series of clinical and metabolic abnormalities known as metabolic syndrome. We identified expression profiles through a coexpression network. In addition, a similarity matrix and expression modules were constructed based on domain and p.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.August.31.5
- DOI:
- 10.4238/2012.August.31.5
cDNA, genomic sequence cloning and overexpression of giant panda (Ailuropoda melanoleuca) mitochondrial ATP synthase ATP5G1
Authors: W.-R. Hou Y.-L. Hou X. Ding T. Wang
The ATP5G1 gene is one of the three genes that encode mitochondrial ATP synthase subunit c of the proton channel. We cloned the cDNA and determined the genomic sequence of the ATP5G1 gene from the giant panda (Ailuropoda melanoleuca) using RT-PCR technology and touchdown-PCR, respectively. The cloned cDNA fragment contains an open reading frame of 411 bp .. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.3.5
- DOI:
- 10.4238/2012.September.3.5
Cloning and prokaryotic expression of rat homolog of Serpina3n and its expression change during liver regeneration
Authors: G.P. Wang X.S. Zhang Y.H. Li J.L. Zheng C.Z. Tang W.X. Zhang
A strikingly upregulated expressed sequence tag was screened from regenerating rat liver at 8 h in a 0-4-8-12 h short-interval successive partial hepatectomy model from a previous study. In the present study, a full-length open reading frame (ORF) corresponding to this expressed sequence tag was predicted through electronic cloning and was subsequently cl.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.3.6
- DOI:
- 10.4238/2012.September.3.6
Gene expression and enzymatic activity of pectin methylesterase during fruit development and ripening in Coffea arabica L.
Authors: S.M.B. Ca�§�£o T.F. Leite I.G.F. Budzinski T.B. dos Santos M.B.S. Scholz V. Carpentieri-Pipolo D.S. Domingues L.G.E. Vieira L.F.P. Pereira
Coffee quality is directly related to the harvest and post harvest conditions. Non-uniform maturation of coffee fruits, combined with inadequate harvest, negatively affects the final quality of the product. Pectin methylesterase (PME) plays an important role in fruit softening due to the hydrolysis of methylester groups in cell wall pectins. In order to c.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.3.7
- DOI:
- 10.4238/2012.September.3.7
Dithranol downregulates expression of Id1 mRNA in human keratinocytes in vitro
Authors: C. Ronpirin T. Tencomnao
The precise causes of psoriasis, a chronic skin disorder characterized by hyperproliferation of keratinocytes and incomplete keratinization, are unclear. It is known that expression of helix-loop-helix transcription factor Id1, which functions as an inhibitor of differentiation, is upregulated in psoriatic skin. We investigated the effect of the antipsori.. Read More»
- Genet. Mol. Res. 11(3):
- 2012.September.12.12
- DOI:
- 10.4238/2012.September.12.12
Expression of PCA3 and PSA genes as a biomarker for differential diagnosis of nodular hyperplasia and prostate cancer
Authors: F. Fonseca Coelho, F. Loli Guimar�£es, W.L. Ribeiro Cabral, P.G. Oliveira Salles, E. Cueva Mateo, L. Mendes Nogueira e Nogueira, C.E. Corradi Fonseca and K. Braga Gomes
We evaluated the expression of the PCA3 gene in urine from patients with nodular hyperplasia/benign prostatic hyperplasia (PNH) or adenocarcinoma type prostate cancer (PCa).The study included 59 men: 22 with PCa, 26 with PNH, and 11 with no alterations (controls). Patients’ urine was collected following prostatic massage and quantified by quantitati.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.13
- DOI:
- 10.4238/2015.October.28.13
Supplemental leucine and isoleucine affect expression of cationic amino acid transporters and myosin, serum concentration of amino acids, and growth performance of pigs
Authors: M. Cervantes-RamÃ?Ârez V. Mendez-Trujillo B.A. Araiza-PiÃ?±a M.A. Barrera-Silva D. GonzÃ?¡lez-Mendoza A. Morales-Trejo
Leucine (Leu) participates in the activity of cationic amino acid (aa) transporters. Also, branched-chain aa [Leu, isoleucine (Ile), and valine (Val)] share intestinal transporters for absorption. We conducted an experiment with 16 young pigs (body weight of about 16 kg) to determine whether Leu and Ile affect expression of aa transporters b0,+ and CAT-1 .. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.3
- DOI:
- 10.4238/2013.January.24.3
Transcriptional activity of an ovarian-specific promoter from rat in dairy goat granulosa cells
Authors: H.-Y. Liu M.-M. Yang Y.-H. Cui T. Ma X.-Q. Liu L. Bai W.-P. Xiao H.-B. Zhao J.-Y. Peng X.-B. Hu B.-Y. Cao
Ovarian-specific promoter 1 (OSP-1) is a retrovirus-like element isolated from the complementary DNA library of rat that has been thought to be specifically expressed in ovary. To exploit this promoter in dairy goat ovary granulosa cells (GCs), OSP-1 from rat was used to construct the reporter vector pOSP-1-EGFP, in which egfp coding for enhanced green fl.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.4
- DOI:
- 10.4238/2013.January.24.4