Expression
Effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus
Authors: Q.D. Jiang1,2 and X.P. Li1
The hypothalamus is an important component of the nervous system, and neuropeptide Y (NPY), proopiomelanocortin (POMC), and neuromedin U (NMU) are key players in physiological regulation. Puerarin is important for nerve regulation. We investigated the effect of puerarin on the expression of NMU, NPY, and POMC genes in the hypothalamus. The results showed .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017362
- DOI:
- 10.4238/gmr.15017362
Efficient isolation of high-quality RNA from lotus Nelumbo nucifera ssp nucifera tissues
Authors: Y.J. Zhang X.Y. Hao Z.S. Liang W.D. Ke H.B. Guo
Nelumbo nucifera is widely used as food, as an ornamental, in medicine, and as packing material; it is also reported to have anti-HIV effects and antioxidant capacity. We sought an improved method for extracting high-quality total RNA from different tissues of N. nucifera. Four methods for RNA extraction were assessed for their ability to recover high-qua.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.January.24.14
- DOI:
- 10.4238/2013.January.24.14
Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA
Authors: J.B. Cajazeiras, L.M. Melo, E.S. Albuquerque, G. RĂ?¡dis-Baptista, B.S. Cavada and V.J.F. Freitas
Low purification efficiency and incomplete characterization of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion.. Read More»
- Genet. Mol. Res. 8(3):
- vol8-3gmr639
- DOI:
- 10.4238/vol8-3gmr639
Effects of exogenous protease supplementation on endogenous trypsin activity and gene expression in broilers
Authors: L. Yuan, S.Q. Wang, Z.X. Wang, H. Zhu and K. Huang
Two hundred and forty one-day-old male broiler chickens (Ross-308) were fed corn-soybean basal diets with 0, 40, 80, or 160 mg/kg of an acid protease preparation from 1 to 42 days of age, in order to investigate the effects of an exogenous enzyme on growth performance, pancreatic trypsin activity and mRNA expression. Average daily gain (ADG) was greatest .. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.28.25
- DOI:
- 10.4238/2015.October.28.25
Characterization of a rice germin-like protein gene promoter
Authors: T. Mahmood T. Yasmin M.I. Haque S.M.S. Naqvi
Germin and germin-like proteins (GLPs) are water-soluble extracellular proteins that are expressed in response to specific environmental and developmental signals. Although some enzymatic activities have also been associated with germin/GLPs, their role in overall metabolism is not fully understood. However, insight into their function may be gained by an.. Read More»
- Genet. Mol. Res. 12(1):
- 2013.February.7.6
- DOI:
- 10.4238/2013.February.7.6
Comparative study of 13 candidate genes applying multi-reference normalization to detect the expression of different fineness in skin tissues of wool sheep
Authors: Y.Z. Tian, T. Usman, K.C. Tian, J. Di, X.X. Huang, X.M. Xu, H. Tulafu, W.W. Wu, X.F. Fu, Y. Bai, M. Tuerxun and A. Maimaiti
Fiber diameter is a useful indicator of wool traits and it is the main determinant of wool quality and value. A comparative study was conducted to analyze the abundance and expression of 13 candidate genes using expression profile microarray analysis and to identify novel molecular markers associated with wool traits to provid.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16018905
- DOI:
- 10.4238/gmr16018905
Expression of macrophage migration-inhibitory factor in duodenal ulcer and its relation to Helicobacter pylori infection
Authors: X.H. Yu, Q. Zhang, X.P. Yang, W. Yang, F. Dai, Z. Qian, Z.L. Wang, C.F. Wu, H.Z. Zhao and G.H. Wang
The aim of this study was to examine the expression of macrophage migration-inhibitory factor (MIF) in duodenal ulcer epithelial cells and its relation to Helicobacter pylori (Hp) infection, and to discuss the pathogenic roles of MIF expression and Hp infection in duodenal ulcer. MIF protein and mRNA expression was examined in samples from patients with d.. Read More»
- Genet. Mol. Res. 14(4):
- 2015.October.29.6
- DOI:
- 10.4238/2015.October.29.6
Differential expression of hENT1 and hENT2 in colon cancer cell lines
Authors: Y. Liu, T. Zuo, X. Zhu, N. Ahuja and T. Fu
Human equilibrative nucleoside transporters (hENT) 1 and 2, encoded by SLC29A1 and SLC29A2, permit the bidirectional passage of nucleoside analogues into cells and may correlate with clinical responses to chemotherapy in patients with colorectal cancer (CRC). The purpose of this study was to evaluate the expression profiles of.. Read More»
- Genet. Mol. Res. 16(1):
- gmr16019549
- DOI:
- 10.4238/gmr16019549
CsSAD: a fatty acid desaturase gene involved in abiotic resistance in Camellia sinensis (L.)
Authors: Z.T. Ding1,2*, J.Z. Shen1,2*, L.L. Pan1,2, Y.U. Wang1,2, Y.S. Li3, Y. Wang4 andH.W. Sun5
Tea (Camellia sinensis L.) is a thermophilic evergreen woody plant that has poor cold tolerance. The SAD gene plays a key role in regulating fatty acid synthesis and membrane lipid fluidity in response to temperature change. In this study, full-length SAD cDNA was cloned from tea leaves using rapid amplification of cDNA ends and polymerase chain reaction .. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017512
- DOI:
- 10.4238/gmr.15017512
Differential expression of FUT1 and FUT2 in Large White, Meishan, and Sutai porcine breeds
Authors: R.W. Xia1, W.Y. Qin1, L.N. Gan1, J. Wang1, S.L. Wu1,2 and W.B. Bao1,2
To assess the relationship between the expression of a(1,2)-fucosyltransferase (FUT1 and FUT2) genes and resistance to Escherichia coli F18 in weaned pigs, FUT1 and FUT2 expression levels in Large White, Meishan, and Sutai pigs (with resistance to E. coli F18) were determined using real-time PCR. The results revealed that FUT1 and FUT2 expression levels w.. Read More»
- Genet. Mol. Res. 15(1):
- gmr.15017613
- DOI:
- 10.4238/gmr.15017613